Comparative Analysis of SARS-CoV-2 Antigenicity across Assays and in Human and Animal Model Sera.

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
27 Sep 2023
Historique:
medline: 9 10 2023
pubmed: 9 10 2023
entrez: 9 10 2023
Statut: epublish

Résumé

The antigenic evolution of SARS-CoV-2 requires ongoing monitoring to judge the immune escape of newly arising variants. A surveillance system necessitates an understanding of differences in neutralization titers measured in different assays and using human and animal sera. We compared 18 datasets generated using human, hamster, and mouse sera, and six different neutralization assays. Titer magnitude was lowest in human, intermediate in hamster, and highest in mouse sera. Fold change, immunodominance patterns and antigenic maps were similar among sera. Most assays yielded similar results, except for differences in fold change in cytopathic effect assays. Not enough data was available for conclusively judging mouse sera, but hamster sera were a consistent surrogate for human first-infection sera.

Identifiants

DOI: 10.1101/2023.09.27.559689 PMID: 37808679 PMC: PMC10557678
pubmed: 37808679
doi: 10.1101/2023.09.27.559689
pmc: PMC10557678
pii:
doi:

Types de publication

Preprint

Langues

eng

Déclaration de conflit d'intérêts

VMC: Named on patents regarding SARS-CoV-2 serological testing and monoclonal antibodies. MSD: Consultant for Inbios, Vir Biotechnology, Ocugen, Topspin Therapeutics, Moderna, and Immunome. The Diamond laboratory has received unrelated funding support in sponsored research agreements from Moderna, Vir Biotechnology, Generate Biomedicines, and Emergent BioSolutions. YK: Received unrelated funding support from Daiichi Sankyo Pharmaceutical, Toyama Chemical, Tauns Laboratories, Inc., Shionogi & Co. LTD, Otsuka Pharmaceutical, KM Biologics, Kyoritsu Seiyaku, Shinya Corporation, and Fuji Rebio. IE: Research grant and speakers fees from Moderna. BMe: Research grant from Moderna. GRS: Is on the GSK Vaccines Scientific Advisory Board. Oxford University holds intellectual property related to the Oxford-AstraZeneca vaccine. MS: Serves in an advisory role for Ocugen, Inc. SP: Reports that the Uniformed Services University (USU) Infectious Diseases Clinical Research Program (IDCRP), a US Department of Defense institution, and the Henry M. Jackson Foundation (HJF) were funded under a Cooperative Research and Development Agreement to conduct an unrelated phase III COVID-19 monoclonal antibody immunoprophylaxis trial sponsored by AstraZeneca. The HJF, in support of the USU IDCRP, was funded by the Department of Defense Joint Program Executive Office for Chemical, Biological, Radiological, and Nuclear Defense to augment the conduct of an unrelated phase III vaccine trial sponsored by AstraZeneca. Both trials were part of the U.S. Government COVID-19 response. Neither is related to the work presented here.

Auteurs

Barbara Mühlemann (B)

Institute of Virology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany.
German Centre for Infection Research (DZIF), partner site Charité, 10117 Berlin, Germany.

Samuel H Wilks (SH)

Center for Pathogen Evolution, Department of Zoology, University of Cambridge, Cambridge, CB2 3EJ, UK.

Lauren Baracco (L)

Center for Pathogen Research, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Meriem Bekliz (M)

Department of Medicine, Faculty of Medicine, University of Geneva, Switzerland.
Centre for Emerging Viral Diseases, University Hospitals of Geneva and University of Geneva, Switzerland.

Juan Manuel Carreño (JM)

Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Victor M Corman (VM)

Institute of Virology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany.
German Centre for Infection Research (DZIF), partner site Charité, 10117 Berlin, Germany.

Meredith E Davis-Gardner (ME)

Department of Pediatrics, Emory Vaccine Center, Emory National Primate Research Center, Emory University School of Medicine, Atlanta, GA, USA.

Wanwisa Dejnirattisai (W)

Wellcome Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7BN, UK.
Division of Emerging Infectious Disease, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkoknoi, Bangkok 10700, Thailand.

Michael S Diamond (MS)

Departments of Medicine, Molecular Microbiology, Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.
Andrew M. and Jane M. Bursky the Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO, USA.
Center for Vaccines and Immunity to Microbial Pathogens, Washington University School of Medicine, St. Louis, MO, USA.

Daniel C Douek (DC)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Christian Drosten (C)

Institute of Virology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany.
German Centre for Infection Research (DZIF), partner site Charité, 10117 Berlin, Germany.

Isabella Eckerle (I)

Department of Medicine, Faculty of Medicine, University of Geneva, Switzerland.
Centre for Emerging Viral Diseases, University Hospitals of Geneva and University of Geneva, Switzerland.
Division of Infectious Diseases, Geneva University Hospitals, Geneva, Switzerland.

Venkata-Viswanadh Edara (VV)

Department of Pediatrics, Emory Vaccine Center, Emory National Primate Research Center, Emory University School of Medicine, Atlanta, GA, USA.

Madison Ellis (M)

Department of Pediatrics, Emory Vaccine Center, Emory National Primate Research Center, Emory University School of Medicine, Atlanta, GA, USA.

Ron A M Fouchier (RAM)

Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands.

Matthew Frieman (M)

Center for Pathogen Research, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Sucheta Godbole (S)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Bart Haagmans (B)

Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands.

Peter J Halfmann (PJ)

Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.

Amy R Henry (AR)

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Terry C Jones (TC)

Institute of Virology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany.
German Centre for Infection Research (DZIF), partner site Charité, 10117 Berlin, Germany.
Center for Pathogen Evolution, Department of Zoology, University of Cambridge, Cambridge, CB2 3EJ, UK.

Leah C Katzelnick (LC)

Viral Epidemiology and Immunity Unit, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Yoshihiro Kawaoka (Y)

Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Division of Virology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
The Research Center for Global Viral Diseases, National Center for Global Health and Medicine Research Institute, Tokyo 162-8655, Japan.
Pandemic Preparedness, Infection and Advanced Research Center (UTOPIA), University of Tokyo, Tokyo 162-8655, Japan.

Janine Kimpel (J)

Institute of Virology, Department of Hygiene, Microbiology and Public Health, Medical University of Innsbruck, Peter-Mayr-Str. 4b, 6020 Innsbruck, Austria.

Florian Krammer (F)

Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Department of Pathology, Cellular and Molecular Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Lilin Lai (L)

Department of Pediatrics, Emory Vaccine Center, Emory National Primate Research Center, Emory University School of Medicine, Atlanta, GA, USA.

Chang Liu (C)

Wellcome Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7BN, UK.
Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.

Sabrina Lusvarghi (S)

Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD 20903, USA.

Benjamin Meyer (B)

Centre of Vaccinology, Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland.

Juthathip Mongkolsapaya (J)

Wellcome Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7BN, UK.
Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.

David C Montefiori (DC)

Department of Surgery, Duke University School of Medicine, Durham, NC, USA.
Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC, USA.

Anna Mykytyn (A)

Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands.

Antonia Netzl (A)

Center for Pathogen Evolution, Department of Zoology, University of Cambridge, Cambridge, CB2 3EJ, UK.

Simon Pollett (S)

Infectious Disease Clinical Research Program, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD, USA.

Annika Rössler (A)

Institute of Virology, Department of Hygiene, Microbiology and Public Health, Medical University of Innsbruck, Peter-Mayr-Str. 4b, 6020 Innsbruck, Austria.

Gavin R Screaton (GR)

Wellcome Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7BN, UK.
Oxford University Hospitals NHS Foundation Trust, Oxford, UK.

Xiaoying Shen (X)

Department of Surgery, Duke University School of Medicine, Durham, NC, USA.
Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC, USA.

Alex Sigal (A)

Africa Health Research Institute, Durban, South Africa.
School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, South Africa.
Centre for the AIDS Programme of Research in South Africa, Durban, South Africa.

Viviana Simon (V)

Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Department of Pathology, Cellular and Molecular Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
The Global Health and Emerging Pathogen Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Rahul Subramanian (R)

Office of Data Science and Emerging Technologies, Office of Science Management and Operations, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Piyada Supasa (P)

Wellcome Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7BN, UK.

Mehul Suthar (M)

Department of Pediatrics, Emory Vaccine Center, Emory National Primate Research Center, Emory University School of Medicine, Atlanta, GA, USA.

Sina Türeli (S)

Center for Pathogen Evolution, Department of Zoology, University of Cambridge, Cambridge, CB2 3EJ, UK.

Wei Wang (W)

Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD 20903, USA.

Carol D Weiss (CD)

Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD 20903, USA.

Derek J Smith (DJ)

Center for Pathogen Evolution, Department of Zoology, University of Cambridge, Cambridge, CB2 3EJ, UK.

Classifications MeSH