Discovery of plasma proteins associated with ventricular fibrillation during first ST-elevation myocardial infarction via proteomics.

Mass spectrometry (MS) Myocardial infarction Proteomics ST-elevation myocardial infarction (STEMI) Ventricular fibrillation (VF)

Journal

European heart journal. Acute cardiovascular care
ISSN: 2048-8734
Titre abrégé: Eur Heart J Acute Cardiovasc Care
Pays: England
ID NLM: 101591369

Informations de publication

Date de publication:
09 Oct 2023
Historique:
received: 03 08 2023
revised: 26 08 2023
accepted: 06 10 2023
medline: 9 10 2023
pubmed: 9 10 2023
entrez: 9 10 2023
Statut: aheadofprint

Résumé

The underlying biological mechanisms of ventricular fibrillation (VF) during acute myocardial infarction are largely unknown. To our knowledge, this is the first proteomic study for this trait, with the aim to identify and characterize proteins that are associated with VF during first ST-elevation myocardial infarction (STEMI). We included 230 participants from a Danish ongoing case-control study on patients with first STEMI with VF (case, n = 110) and without VF (control, n = 120) before guided catheter insertion for primary percutaneous coronary intervention. The plasma proteome was investigated using mass spectrometry-based proteomics on plasma samples collected within 24 hours of symptom onset. In 229 STEMI patients (72% men, median age 62 years (interquartile range (IQR): 54-70)), a median of 257 proteins (IQR: 244-281) were quantified per patient. A total of 26 proteins were associated with VF, these proteins were involved in several biological processes including blood coagulation, hemostasis, and immunity. After correcting for multiple testing, two up-regulated proteins remained significantly associated with VF, actin beta-like 2 (ACTBL2, fold-change (FC) 2.25, p < 0.001, q = 0.023) and coagulation factor XIII-A (F13A1, FC 1.48, p < 0.001, q = 0.023). None of the proteins were correlated with anterior infarct location. VF due to first STEMI was significantly associated with two up-regulated proteins (ACTBL2 and F13A1), suggesting that they may represent novel underlying molecular VF mechanisms. Further research is needed to determine whether these proteins are predictive biomarkers or acute phase response proteins to VF during acute ischemia.

Sections du résumé

BACKGROUND AND AIMS OBJECTIVE
The underlying biological mechanisms of ventricular fibrillation (VF) during acute myocardial infarction are largely unknown. To our knowledge, this is the first proteomic study for this trait, with the aim to identify and characterize proteins that are associated with VF during first ST-elevation myocardial infarction (STEMI).
METHODS METHODS
We included 230 participants from a Danish ongoing case-control study on patients with first STEMI with VF (case, n = 110) and without VF (control, n = 120) before guided catheter insertion for primary percutaneous coronary intervention. The plasma proteome was investigated using mass spectrometry-based proteomics on plasma samples collected within 24 hours of symptom onset.
RESULTS RESULTS
In 229 STEMI patients (72% men, median age 62 years (interquartile range (IQR): 54-70)), a median of 257 proteins (IQR: 244-281) were quantified per patient. A total of 26 proteins were associated with VF, these proteins were involved in several biological processes including blood coagulation, hemostasis, and immunity. After correcting for multiple testing, two up-regulated proteins remained significantly associated with VF, actin beta-like 2 (ACTBL2, fold-change (FC) 2.25, p < 0.001, q = 0.023) and coagulation factor XIII-A (F13A1, FC 1.48, p < 0.001, q = 0.023). None of the proteins were correlated with anterior infarct location.
CONCLUSION CONCLUSIONS
VF due to first STEMI was significantly associated with two up-regulated proteins (ACTBL2 and F13A1), suggesting that they may represent novel underlying molecular VF mechanisms. Further research is needed to determine whether these proteins are predictive biomarkers or acute phase response proteins to VF during acute ischemia.

Identifiants

pubmed: 37811694
pii: 7301242
doi: 10.1093/ehjacc/zuad125
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Commentaires et corrections

Type : CommentIn

Informations de copyright

© The Author(s) 2023. Published by Oxford University Press on behalf of the European Society of Cardiology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Auteurs

Niels Kjær Stampe (NK)

Department of Cardiology, The Heart Centre, Copenhagen University Hospital - Rigshospitalet, Denmark.

Maud Eline Ottenheijm (ME)

NNF Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen.
Department of Clinical Biochemistry, Copenhagen University Hospital Bispebjerg Hospital, Copenhagen, Denmark.

Lylia Drici (L)

NNF Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen.
Department of Clinical Biochemistry, Copenhagen University Hospital Bispebjerg Hospital, Copenhagen, Denmark.

Nicolai J Wewer Albrechtsen (NJ)

NNF Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen.
Department of Clinical Biochemistry, Copenhagen University Hospital Bispebjerg Hospital, Copenhagen, Denmark.

Annelaura Bach Nielsen (AB)

NNF Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen.
Department of Clinical Biochemistry, Copenhagen University Hospital Bispebjerg Hospital, Copenhagen, Denmark.

Christina Christoffersen (C)

Department of Clinical Biochemistry, Centre of Diagnostic Investigation, Copenhagen University Hospital - Rigshospitalet, Denmark.
Department of Biomedical Sciences, Faculty of Medical Sciences, University of Copenhagen, Denmark.

Peder Emil Warming (PE)

Department of Cardiology, The Heart Centre, Copenhagen University Hospital - Rigshospitalet, Denmark.

Thomas Engstrøm (T)

Department of Cardiology, The Heart Centre, Copenhagen University Hospital - Rigshospitalet, Denmark.

Bo Gregers Winkel (BG)

Department of Cardiology, The Heart Centre, Copenhagen University Hospital - Rigshospitalet, Denmark.

Reza Jabbari (R)

Department of Cardiology, The Heart Centre, Copenhagen University Hospital - Rigshospitalet, Denmark.

Jacob Tfelt-Hansen (J)

Department of Cardiology, The Heart Centre, Copenhagen University Hospital - Rigshospitalet, Denmark.
Department of Forensic Medicine, Faculty of Medical Sciences, University of Copenhagen, Denmark.

Charlotte Glinge (C)

Department of Cardiology, The Heart Centre, Copenhagen University Hospital - Rigshospitalet, Denmark.

Classifications MeSH