Evaluated periodontal tissues and oxidative stress in rats with neuropathic pain-like behavior.
Humans
Rats
Male
Animals
Rats, Sprague-Dawley
Alveolar Bone Loss
Oxidative Stress
Antioxidants
/ metabolism
8-Hydroxy-2'-Deoxyguanosine
/ metabolism
Paclitaxel
/ pharmacology
Neuralgia
/ genetics
Periodontal Ligament
/ metabolism
Superoxide Dismutase
/ genetics
NAV1.7 Voltage-Gated Sodium Channel
/ metabolism
Chronic pain
Nociceptive sensitivity
Oxidative stress
Periodontal disease
SCN9A
TRPA1
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
21
07
2023
accepted:
15
09
2023
medline:
10
11
2023
pubmed:
9
10
2023
entrez:
9
10
2023
Statut:
ppublish
Résumé
Oxidative stress has a critical effect on both persistent pain states and periodontal disease. Voltage-gated sodium NaV1.7 (SCN9A), and transient receptor potential ankyrin 1 (TRPA1) are pain genes. The goal of this study was to investigate oxidative stress markers, periodontal status, SCN9A, and TRPA1 channel expression in periodontal tissues of rats with paclitaxel-induced neuropathic pain-like behavior (NPLB). Totally 16 male Sprague Dawley rats were used: control (n = 8) and paclitaxel-induced pain (PTX) (n = 8). The alveolar bone loss and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels were analyzed histometrically and immunohistochemically. Gingival superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities (spectrophotometric assay) were measured. The relative TRPA1 and SCN9A genes expression levels were evaluated using quantitative real-time PCR (qPCR) in the tissues of gingiva and brain. The PTX group had significantly higher alveolar bone loss and 8-OHdG compared to the control. The PTX group had significantly lower gingival SOD, GPx and CAT activity than the control groups. The PTX group had significantly higher relative gene expression of SCN9A (p = 0.0002) and TRPA1 (p = 0.0002) than the control in gingival tissues. Increased nociceptive susceptibility may affect the increase in oxidative stress and periodontal destruction. Chronic pain conditions may increase TRPA1 and SCN9A gene expression in the periodontium. The data of the current study may help develop novel approaches both to maintain periodontal health and alleviate pain in patients suffering from orofacial pain.
Sections du résumé
BACKGROUND
BACKGROUND
Oxidative stress has a critical effect on both persistent pain states and periodontal disease. Voltage-gated sodium NaV1.7 (SCN9A), and transient receptor potential ankyrin 1 (TRPA1) are pain genes. The goal of this study was to investigate oxidative stress markers, periodontal status, SCN9A, and TRPA1 channel expression in periodontal tissues of rats with paclitaxel-induced neuropathic pain-like behavior (NPLB).
METHODS AND RESULTS
RESULTS
Totally 16 male Sprague Dawley rats were used: control (n = 8) and paclitaxel-induced pain (PTX) (n = 8). The alveolar bone loss and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels were analyzed histometrically and immunohistochemically. Gingival superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities (spectrophotometric assay) were measured. The relative TRPA1 and SCN9A genes expression levels were evaluated using quantitative real-time PCR (qPCR) in the tissues of gingiva and brain. The PTX group had significantly higher alveolar bone loss and 8-OHdG compared to the control. The PTX group had significantly lower gingival SOD, GPx and CAT activity than the control groups. The PTX group had significantly higher relative gene expression of SCN9A (p = 0.0002) and TRPA1 (p = 0.0002) than the control in gingival tissues. Increased nociceptive susceptibility may affect the increase in oxidative stress and periodontal destruction.
CONCLUSIONS
CONCLUSIONS
Chronic pain conditions may increase TRPA1 and SCN9A gene expression in the periodontium. The data of the current study may help develop novel approaches both to maintain periodontal health and alleviate pain in patients suffering from orofacial pain.
Identifiants
pubmed: 37812355
doi: 10.1007/s11033-023-08829-z
pii: 10.1007/s11033-023-08829-z
doi:
Substances chimiques
Antioxidants
0
8-Hydroxy-2'-Deoxyguanosine
88847-89-6
Paclitaxel
P88XT4IS4D
Superoxide Dismutase
EC 1.15.1.1
SCN9A protein, human
0
NAV1.7 Voltage-Gated Sodium Channel
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9315-9322Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
Références
Kaye AD, Jones MR, Kaye AM et al (2017) Prescription opioid abuse in Chronic Pain: an updated review of opioid abuse predictors and strategies to curb opioid abuse: part 1. Pain Physician 20:S93–S109
doi: 10.36076/ppj.2017.s109
pubmed: 28226333
Latremoliere A, Woolf CJ (2009) Central sensitization: a generator of pain hypersensitivity by central neural plasticity. J Pain 10:895–926. https://doi.org/10.1016/j.jpain.2009.06.012
doi: 10.1016/j.jpain.2009.06.012
pubmed: 19712899
pmcid: 2750819
Fornasari D (2012) Pain mechanisms in patients with chronic pain. Clin Drug Investig 32(Suppl 1):45–52. https://doi.org/10.2165/11630070-000000000-00000
doi: 10.2165/11630070-000000000-00000
pubmed: 23389875
Treede RD, Rief W, Barke A et al (2019) Chronic pain as a symptom or a disease: the IASP classification of Chronic Pain for the International classification of Diseases (ICD-11). Pain 160:19–27. https://doi.org/10.1097/j.pain.0000000000001384
doi: 10.1097/j.pain.0000000000001384
pubmed: 30586067
Harper DE, Schrepf A, Clauw DJ (2016) Pain Mechanisms and Centralized Pain in Temporomandibular Disorders. J Dent Res 95:1102–1108. https://doi.org/10.1177/0022034516657070
doi: 10.1177/0022034516657070
pubmed: 27422858
pmcid: 5004242
Mannerak MA, Lashkarivand A, Eide PK (2021) Trigeminal neuralgia and genetics: a systematic review. Mol Pain 17:17448069211016139. https://doi.org/10.1177/17448069211016139
doi: 10.1177/17448069211016139
pubmed: 34000891
pmcid: 8135221
Shakouri SK, Dolatkhah N, Omidbakhsh S, Pishgahi A, Hashemian M (2020) Serum inflammatory and oxidative stress biomarkers levels are associated with pain intensity, pressure pain threshold and quality of life in myofascial pain syndrome. BMC Res Notes 13:510. https://doi.org/10.1186/s13104-020-05352-3
doi: 10.1186/s13104-020-05352-3
pubmed: 33160410
pmcid: 7648320
Salvemini D, Little JW, Doyle T, Neumann WL (2011) Roles of reactive oxygen and nitrogen species in pain. Free Radic Biol Med 51:951–966. https://doi.org/10.1016/j.freeradbiomed.2011.01.026
doi: 10.1016/j.freeradbiomed.2011.01.026
pubmed: 21277369
pmcid: 3134634
Park ES, Gao X, Chung JM, Chung K (2006) Levels of mitochondrial reactive oxygen species increase in rat neuropathic spinal dorsal horn neurons. Neurosci Lett 391:108–111. https://doi.org/10.1016/j.neulet.2005.08.055
doi: 10.1016/j.neulet.2005.08.055
pubmed: 16183198
Herzberg D, Strobel P, Chihuailaf R et al (2019) Spinal reactive oxygen species and oxidative damage Mediate Chronic Pain in Lame dairy cows. Anim (Basel) 9. https://doi.org/10.3390/ani9090693
Babior BM (2000) Phagocytes and oxidative stress. Am J Med 109:33–44. https://doi.org/10.1016/s0002-9343(00)00481-2
doi: 10.1016/s0002-9343(00)00481-2
pubmed: 10936476
Karaman M, Toraman E, Sulukan E et al (2023) Fluoride exposure causes behavioral, molecular and physiological changes in adult zebrafish (Danio rerio) and their offspring. Environ Toxicol Pharmacol 97:104044. https://doi.org/10.1016/j.etap.2022.104044
doi: 10.1016/j.etap.2022.104044
pubmed: 36566951
Chapple IL, Matthews JB (2007) The role of reactive oxygen and antioxidant species in periodontal tissue destruction. Periodontol 2000 43:160–232. https://doi.org/10.1111/j.1600-0757.2006.00178.x
doi: 10.1111/j.1600-0757.2006.00178.x
pubmed: 17214840
Na HJ, Kim OS, Park BJ (2006) Expression of Superoxide dismutase isoforms in inflamed gingiva. J Korean Acad Periodontol 36:97–112
doi: 10.5051/jkape.2006.36.1.97
Akalin FA, Toklu E, Renda N (2005) Analysis of superoxide dismutase activity levels in gingiva and gingival crevicular fluid in patients with chronic periodontitis and periodontally healthy controls. J Clin Periodontol 32:238–243. https://doi.org/10.1111/j.1600-051X.2005.00669.x
doi: 10.1111/j.1600-051X.2005.00669.x
pubmed: 15766365
Sczepanik FSC, Grossi ML, Casati M et al (2020) Periodontitis is an inflammatory disease of oxidative stress: we should treat it that way. Periodontol 2000 84:45–68. https://doi.org/10.1111/prd.12342
doi: 10.1111/prd.12342
pubmed: 32844417
Garrett IR, Boyce BF, Oreffo RO, Bonewald L, Poser J, Mundy GR (1990) Oxygen-derived free radicals stimulate osteoclastic bone resorption in rodent bone in vitro and in vivo. J Clin Invest 85:632–639. https://doi.org/10.1172/JCI114485
doi: 10.1172/JCI114485
pubmed: 2312718
pmcid: 296476
Koori K, Maeda H, Fujii S et al (2014) The roles of calcium-sensing receptor and calcium channel in osteogenic differentiation of undifferentiated periodontal ligament cells. Cell Tissue Res 357:707–718. https://doi.org/10.1007/s00441-014-1918-5
doi: 10.1007/s00441-014-1918-5
pubmed: 24842051
Rifkin BR, Vernillo AT, Golub LM (1993) Blocking Periodontal Disease Progression by inhibiting tissue-destructive enzymes: a potential therapeutic role for tetracyclines and their chemically-modified analogs. J Periodontol 64:819–827. https://doi.org/10.1902/jop.1993.64.8s.819
doi: 10.1902/jop.1993.64.8s.819
pubmed: 29539753
Souza Monteiro de Araujo D, Nassini R, Geppetti P, De Logu F (2020) TRPA1 as a therapeutic target for nociceptive pain. Expert Opin Ther Targets 24:997–1008. https://doi.org/10.1080/14728222.2020.1815191
doi: 10.1080/14728222.2020.1815191
pubmed: 32838583
pmcid: 7610834
Kobayashi K, Fukuoka T, Obata K et al (2005) Distinct expression of TRPM8, TRPA1, and TRPV1 mRNAs in rat primary afferent neurons with adelta/c-fibers and colocalization with trk receptors. J Comp Neurol 493:596–606. https://doi.org/10.1002/cne.20794
doi: 10.1002/cne.20794
pubmed: 16304633
Trevisani M, Siemens J, Materazzi S et al (2007) 4-Hydroxynonenal, an endogenous aldehyde, causes pain and neurogenic inflammation through activation of the irritant receptor TRPA1. Proc Natl Acad Sci U S A 104:13519–13524. https://doi.org/10.1073/pnas.0705923104
doi: 10.1073/pnas.0705923104
pubmed: 17684094
pmcid: 1948902
Sawada Y, Hosokawa H, Matsumura K, Kobayashi S (2008) Activation of transient receptor potential ankyrin 1 by hydrogen peroxide. Eur J Neurosci 27:1131–1142. https://doi.org/10.1111/j.1460-9568.2008.06093.x
doi: 10.1111/j.1460-9568.2008.06093.x
pubmed: 18364033
Ito M, Ono K, Hitomi S et al (2017) Prostanoid-dependent spontaneous pain and PAR2-dependent mechanical allodynia following oral mucosal trauma: involvement of TRPV1, TRPA1 and TRPV4. Mol Pain 13:1744806917704138. https://doi.org/10.1177/1744806917704138
doi: 10.1177/1744806917704138
pubmed: 28381109
pmcid: 5407658
Nyman E, Franzen B, Nolting A et al (2013) In vitro pharmacological characterization of a novel TRPA1 antagonist and proof of mechanism in a human dental pulp model. J Pain Res 6:59–70. https://doi.org/10.2147/JPR.S37567
doi: 10.2147/JPR.S37567
pubmed: 23403691
pmcid: 3565573
Pusztai L, Mendoza TR, Reuben JM et al (2004) Changes in plasma levels of inflammatory cytokines in response to paclitaxel chemotherapy. Cytokine 25:94–102. https://doi.org/10.1016/j.cyto.2003.10.004
doi: 10.1016/j.cyto.2003.10.004
pubmed: 14698135
Fazekas A, Vindisch K, Posch E, Gyorfi A (1990) Experimentally-induced neurogenic inflammation in the rat oral mucosa. J Periodontal Res 25:276–282. https://doi.org/10.1111/j.1600-0765.1990.tb00916.x
doi: 10.1111/j.1600-0765.1990.tb00916.x
pubmed: 2145413
Son DB, Choi W, Kim M et al (2021) Decursin alleviates mechanical Allodynia in a Paclitaxel-Induced Neuropathic Pain Mouse Model. Cells 10. https://doi.org/10.3390/cells10030547
doi: 10.3390/cells10030547
Yam MF, Loh YC, Oo CW, Basir R (2020) Overview of neurological mechanism of Pain Profile used for Animal Pain-Like behavioral study with proposed analgesic pathways. Int J Mol Sci 21. https://doi.org/10.3390/ijms21124355
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. https://doi.org/10.1006/abio.1976.9999
doi: 10.1006/abio.1976.9999
pubmed: 942051
Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497–500
doi: 10.1093/clinchem/34.3.497
pubmed: 3349599
Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126. https://doi.org/10.1016/s0076-6879(84)05016-3
doi: 10.1016/s0076-6879(84)05016-3
pubmed: 6727660
Beutler E (1975) Red cell metabolism. A manual of biochemical methods: 11–12
Kocpinar EF, Gonul Baltaci N, Ceylan H, Kalin SN, Erdogan O, Budak H (2020) Effect of a prolonged Dietary Iron Intake on the Gene expression and activity of the testicular antioxidant defense system in rats. Biol Trace Elem Res 195:135–141. https://doi.org/10.1007/s12011-019-01817-0
doi: 10.1007/s12011-019-01817-0
pubmed: 31309445
Carrasco C, Naziroglu M, Rodriguez AB, Pariente JA (2018) Neuropathic Pain: delving into the oxidative origin and the possible implication of transient receptor potential channels. Front Physiol 9:95. https://doi.org/10.3389/fphys.2018.00095
doi: 10.3389/fphys.2018.00095
pubmed: 29491840
pmcid: 5817076
Zhao M, Zhang X, Tao X et al (2021) Sirt2 in the spinal cord regulates Chronic Neuropathic Pain through Nrf2-Mediated oxidative stress pathway in rats. Front Pharmacol 12:646477. https://doi.org/10.3389/fphar.2021.646477
doi: 10.3389/fphar.2021.646477
pubmed: 33897435
pmcid: 8063033
Komirishetty P, Areti A, Yerra VG et al (2016) PARP inhibition attenuates neuroinflammation and oxidative stress in chronic constriction injury induced peripheral neuropathy. Life Sci 150:50–60. https://doi.org/10.1016/j.lfs.2016.02.085
doi: 10.1016/j.lfs.2016.02.085
pubmed: 26921631
Guedes RP, Dal Bosco L, Araujo AS, Bello-Klein A, Ribeiro MF, Partata WA (2009) Sciatic nerve transection increases gluthatione antioxidant system activity and neuronal nitric oxide synthase expression in the spinal cord. Brain Res Bull 80:422–427. https://doi.org/10.1016/j.brainresbull.2009.08.007
doi: 10.1016/j.brainresbull.2009.08.007
pubmed: 19683561
Kerckhove N, Collin A, Conde S, Chaleteix C, Pezet D, Balayssac D (2017) Long-Term Effects, pathophysiological mechanisms, and risk factors of Chemotherapy-Induced Peripheral Neuropathies: a Comprehensive Literature Review. Front Pharmacol 8:86. https://doi.org/10.3389/fphar.2017.00086
doi: 10.3389/fphar.2017.00086
pubmed: 28286483
pmcid: 5323411
Ogawa N, Kurokawa T, Mori Y (2016) Sensing of redox status by TRP channels. Cell Calcium 60:115–122. https://doi.org/10.1016/j.ceca.2016.02.009
doi: 10.1016/j.ceca.2016.02.009
pubmed: 26969190
Materazzi S, Fusi C, Benemei S et al (2012) TRPA1 and TRPV4 mediate paclitaxel-induced peripheral neuropathy in mice via a glutathione-sensitive mechanism. Pflugers Arch 463:561–569. https://doi.org/10.1007/s00424-011-1071-x
doi: 10.1007/s00424-011-1071-x
pubmed: 22258694
Markowitz K (2010) Pretty painful: why does tooth bleaching hurt? Med Hypotheses 74:835–840. https://doi.org/10.1016/j.mehy.2009.11.044
doi: 10.1016/j.mehy.2009.11.044
pubmed: 20045265
Kim YS, Jung HK, Kwon TK et al (2012) Expression of transient receptor potential ankyrin 1 in human dental pulp. J Endod 38:1087–1092. https://doi.org/10.1016/j.joen.2012.04.024
doi: 10.1016/j.joen.2012.04.024
pubmed: 22794211
Siqueira SR, Alves B, Malpartida HM, Teixeira MJ, Siqueira JT (2009) Abnormal expression of voltage-gated sodium channels Nav1.7, Nav1.3 and Nav1.8 in trigeminal neuralgia. Neuroscience 164:573–577. https://doi.org/10.1016/j.neuroscience.2009.08.037
doi: 10.1016/j.neuroscience.2009.08.037
pubmed: 19699781
Costa GMF, Rocha LPC, Siqueira S, Moreira PR, Almeida-Leite CM (2019) No association of polymorphisms in Nav1.7 or nerve growth factor receptor genes with trigeminal Neuralgia. Pain Med 20:1362–1369. https://doi.org/10.1093/pm/pny191
doi: 10.1093/pm/pny191
pubmed: 30307573
Korczeniewska OA, Husain S, Khan J, Eliav E, Soteropoulos P, Benoliel R (2018) Differential gene expression in trigeminal ganglia of male and female rats following chronic constriction of the infraorbital nerve. Eur J Pain 22:875–888. https://doi.org/10.1002/ejp.1174
doi: 10.1002/ejp.1174
pubmed: 29350446
Hong SS, Morrow TJ, Paulson PE, Isom LL, Wiley JW (2004) Early painful diabetic neuropathy is associated with differential changes in tetrodotoxin-sensitive and -resistant sodium channels in dorsal root ganglion neurons in the rat. J Biol Chem 279:29341–29350. https://doi.org/10.1074/jbc.M404167200
doi: 10.1074/jbc.M404167200
pubmed: 15123645
Chattopadhyay M, Mata M, Fink DJ (2008) Continuous delta-opioid receptor activation reduces neuronal voltage-gated sodium channel (na(V)1.7) levels through activation of protein kinase C in painful diabetic neuropathy. J Neurosci 28:6652–6658. https://doi.org/10.1523/Jneurosci.5530-07.2008
doi: 10.1523/Jneurosci.5530-07.2008
pubmed: 18579738
pmcid: 3321315
Huang YL, Zang Y, Zhou LJ, Gui WS, Liu XG, Zhong Y (2014) The role of TNF-alpha/NF-kappa B pathway on the up-regulation of voltage-gated sodium channel Nav1.7 in DRG neurons of rats with diabetic neuropathy. Neurochem Int 75:112–119. https://doi.org/10.1016/j.neuint.2014.05.012
doi: 10.1016/j.neuint.2014.05.012
pubmed: 24893330
Toraman E, Bayram C, Sezen S, Ozkaraca M, Hacimuftuoglu A, Budak H (2023) Parthenolide as a potential analgesic in the treatment of paclitaxel-induced neuropathic pain: the rat modeling. https://doi.org/10.1007/s00210-023-02568-5 . Naunyn-Schmiedebergs Archives of Pharmacology
Zhang H, Dougherty PM (2014) Enhanced excitability of primary sensory neurons and altered gene expression of neuronal ion channels in dorsal root ganglion in paclitaxel-induced peripheral neuropathy. Anesthesiology 120:1463–1475. https://doi.org/10.1097/ALN.0000000000000176
doi: 10.1097/ALN.0000000000000176
pubmed: 24534904
Li Y, North RY, Rhines LD et al (2018) DRG voltage-gated Sodium Channel 1.7 is upregulated in Paclitaxel-Induced Neuropathy in rats and in humans with Neuropathic Pain. J Neurosci 38:1124–1136. https://doi.org/10.1523/Jneurosci.0899-17.2017
doi: 10.1523/Jneurosci.0899-17.2017
pubmed: 29255002
pmcid: 5792474
Huang Y, Zang Y, Zhou L, Gui W, Liu X, Zhong Y (2014) The role of TNF-alpha/NF-kappa B pathway on the up-regulation of voltage-gated sodium channel Nav1.7 in DRG neurons of rats with diabetic neuropathy. Neurochem Int 75:112–119. https://doi.org/10.1016/j.neuint.2014.05.012
doi: 10.1016/j.neuint.2014.05.012
pubmed: 24893330
Zhang P, Gan YH (2017) Prostaglandin E2 upregulated trigeminal Ganglionic Sodium Channel 1.7 Involving Temporomandibular Joint Inflammatory Pain in rats. Inflammation 40:1102–1109. https://doi.org/10.1007/s10753-017-0552-2
doi: 10.1007/s10753-017-0552-2
pubmed: 28349234
Borges I, Moreira EAM, Wilhem D, de Oliveira TB, da Silva MBS, Froede AS (2007) Proinflammatory and oxidative stress markers in patients with periodontal disease. Mediators of Inflammation 2007https://doi.org/Artn 4579410.1155/2007/45794
Ying SQ, Tan MM, Feng G et al (2020) Low-intensity pulsed Ultrasound regulates alveolar bone homeostasis in experimental Periodontitis by diminishing oxidative stress. Theranostics 10:9789–9807. https://doi.org/10.7150/thno.42508
doi: 10.7150/thno.42508
pubmed: 32863960
pmcid: 7449900
Carvalho JS, Ramadan D, de Paiva Goncalves V et al (2021) Impact of citrus flavonoid supplementation on inflammation in lipopolysaccharide-induced periodontal disease in mice. Food Funct 12:5007–5017. https://doi.org/10.1039/d0fo03338c
doi: 10.1039/d0fo03338c
pubmed: 33950049
Akalin FA, Isiksal E, Baltacioglu E, Renda N, Karabulut E (2008) Superoxide dismutase activity in gingiva in type-2 diabetes mellitus patients with chronic periodontitis. Arch Oral Biol 53:44–52. https://doi.org/10.1016/j.archoralbio.2007.07.009
doi: 10.1016/j.archoralbio.2007.07.009
pubmed: 17880913
Toraman A, Arabaci T, Aytekin Z, Albayrak M, Bayir Y (2020) Effects of vitamin C local application on ligature-induced periodontitis in diabetic rats. J Appl Oral Sci 28:e20200444. https://doi.org/10.1590/1678-7757-2020-0444
doi: 10.1590/1678-7757-2020-0444
pubmed: 33263670
pmcid: 7695129
DeLeo JA, Rutkowski MD (2000) Gender differences in rat neuropathic pain sensitivity is dependent on strain. Neurosci Lett 282:197–199. https://doi.org/10.1016/s0304-3940(00)00880-6
doi: 10.1016/s0304-3940(00)00880-6
pubmed: 10717425
Dominguez CA, Strom M, Gao T et al (2012) Genetic and sex influence on neuropathic pain-like behaviour after spinal cord injury in the rat. Eur J Pain 16:1368–1377. https://doi.org/10.1002/j.1532-2149.2012.00144.x
doi: 10.1002/j.1532-2149.2012.00144.x
pubmed: 22473909