Single B cell transcriptomics identifies multiple isotypes of broadly neutralizing antibodies against flaviviruses.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
10 2023
10 2023
Historique:
received:
17
05
2023
accepted:
28
09
2023
revised:
19
10
2023
medline:
23
10
2023
pubmed:
9
10
2023
entrez:
9
10
2023
Statut:
epublish
Résumé
Sequential dengue virus (DENV) infections often generate neutralizing antibodies against all four DENV serotypes and sometimes, Zika virus. Characterizing cross-flavivirus broadly neutralizing antibody (bnAb) responses can inform countermeasures that avoid enhancement of infection associated with non-neutralizing antibodies. Here, we used single cell transcriptomics to mine the bnAb repertoire following repeated DENV infections. We identified several new bnAbs with comparable or superior breadth and potency to known bnAbs, and with distinct recognition determinants. Unlike all known flavivirus bnAbs, which are IgG1, one newly identified cross-flavivirus bnAb (F25.S02) was derived from IgA1. Both IgG1 and IgA1 versions of F25.S02 and known bnAbs displayed neutralizing activity, but only IgG1 enhanced infection in monocytes expressing IgG and IgA Fc receptors. Moreover, IgG-mediated enhancement of infection was inhibited by IgA1 versions of bnAbs. We demonstrate a role for IgA in flavivirus infection and immunity with implications for vaccine and therapeutic strategies.
Identifiants
pubmed: 37812640
doi: 10.1371/journal.ppat.1011722
pii: PPATHOGENS-D-23-00811
pmc: PMC10586629
doi:
Substances chimiques
Broadly Neutralizing Antibodies
0
Antibodies, Neutralizing
0
Immunoglobulin G
0
Immunoglobulin A
0
Antibodies, Viral
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1011722Subventions
Organisme : NCI NIH HHS
ID : P30 CA015704
Pays : United States
Organisme : NIH HHS
ID : S10 OD028685
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM129325
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI083203
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI146028
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI057229
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
Copyright: © 2023 Lubow et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
I have read the journal’s policy and the authors of this manuscript have the following competing interests: LG, JL, FAM, DKR, LML are inventors on a patent application filed by Fred Hutchinson Cancer Center relating to newly discovered antibodies described in this paper. SK, ED, BJD are employees of Integral Molecular, Inc.; BJD is also a shareholder of the company.
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