Chromosomal gene of hybrid multisensor histidine kinase is involved in motility regulation in the rhizobacterium Azospirillum baldaniorum Sp245 under mechanical and water stress.
Azospirillum baldaniorum Flagella
Biofilms
Histidine kinase
Mechanosensing
Motility
Osmotic stress
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
10 Oct 2023
10 Oct 2023
Historique:
received:
21
07
2023
accepted:
29
09
2023
medline:
11
10
2023
pubmed:
10
10
2023
entrez:
9
10
2023
Statut:
epublish
Résumé
Azospirillum alphaproteobacteria, which live in the rhizosphere of many crops, are used widely as biofertilizers. Two-component signal transduction systems (TCSs) mediate the bacterial perception of signals and the corresponding adjustment of behavior facilitating the adaptation of bacteria to their habitats. In this study, we obtained the A. baldaniorum Sp245 mutant for the AZOBR_150176 gene, which encodes the TCS of the hybrid histidine kinase/response sensory regulator (HSHK-RR). Inactivation of this gene affected bacterial morphology and motility. In mutant Sp245-HSHKΔRR-Km, the cells were still able to synthesize a functioning polar flagellum (Fla), were shorter than those of strain Sp245, and were impaired in aerotaxis, elaboration of inducible lateral flagella (Laf), and motility in semiliquid media. The mutant showed decreased transcription of the genes encoding the proteins of the secretion apparatus, which ensures the assembly of Laf, Laf flagellin, and the repressor protein of translation of the Laf flagellin's mRNA. The study examined the effects of polyethylene glycol 6000 (PEG 6000), an agent used to simulate osmotic stress and drought conditions. Under osmotic stress, the mutant was no longer able to use collective motility in semiliquid media but formed more biofilm biomass than did strain Sp245. Introduction into mutant cells of the AZOBR_150176 gene as part of an expression vector led to recovery of the lost traits, including those mediating bacterial motility under mechanical stress induced by increased medium density. The results suggest that the HSHK-RR under study modulates the response of A. baldaniorum Sp245 to mechanical and osmotic/water stress.
Identifiants
pubmed: 37814195
doi: 10.1007/s11274-023-03785-z
pii: 10.1007/s11274-023-03785-z
doi:
Substances chimiques
Histidine Kinase
EC 2.7.13.1
Flagellin
12777-81-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
336Subventions
Organisme : Russian Science Foundation
ID : project no. 23-26-00271
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
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doi: 10.1128/jb.178.17.5199-5204.1996
pubmed: 8752338
pmcid: 178317