SIN-3 acts in distinct complexes to regulate the germline transcriptional program in Caenorhabditis elegans.
C. elegans
Germline
HDAC
Proteomics
SIN3
X chromosome
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
01 11 2023
01 11 2023
Historique:
received:
08
03
2023
accepted:
18
09
2023
medline:
23
10
2023
pubmed:
11
10
2023
entrez:
11
10
2023
Statut:
ppublish
Résumé
The transcriptional co-regulator SIN3 influences gene expression through multiple interactions that include histone deacetylases. Haploinsufficiency and mutations in SIN3 are the underlying cause of Witteveen-Kolk syndrome and related intellectual disability and autism syndromes, emphasizing its key role in development. However, little is known about the diversity of its interactions and functions in developmental processes. Here, we show that loss of SIN-3, the single SIN3 homolog in Caenorhabditis elegans, results in maternal-effect sterility associated with de-regulation of the germline transcriptome, including de-silencing of X-linked genes. We identify at least two distinct SIN3 complexes containing specific histone deacetylases and show that they differentially contribute to fertility. Single-cell, single-molecule fluorescence in situ hybridization reveals that in sin-3 mutants the X chromosome becomes re-expressed prematurely and in a stochastic manner in individual germ cells, suggesting a role for SIN-3 in its silencing. Furthermore, we identify histone residues whose acetylation increases in the absence of SIN-3. Together, this work provides a powerful framework for the in vivo study of SIN3 and associated proteins.
Identifiants
pubmed: 37818613
pii: 333417
doi: 10.1242/dev.201755
pmc: PMC10617626
pii:
doi:
Substances chimiques
Histone Deacetylases
EC 3.5.1.98
Histones
0
sin-3 protein, C elegans
EC 3.5.1.98
Caenorhabditis elegans Proteins
0
Sin3 Histone Deacetylase and Corepressor Complex
EC 3.5.1.98
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2023. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
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