Single-shot multi-channel plasmonic real-time polymerase chain reaction for multi-target point-of-care testing.

Humans Real-Time Polymerase Chain Reaction RNA, Viral / genetics Point-of-Care Testing Reverse Transcription Point-of-Care Systems Nucleic Acid Amplification Techniques / methods Sensitivity and Specificity

Journal

Lab on a chip
ISSN: 1473-0189
Titre abrégé: Lab Chip
Pays: England
ID NLM: 101128948

Informations de publication

Date de publication:
24 10 2023
Historique:
medline: 31 10 2023
pubmed: 12 10 2023
entrez: 12 10 2023
Statut: epublish

Résumé

Plasmonic nucleic acid amplification tests demand high-throughput and multi-target detection of infectious diseases as well as short turnaround time and small size for point-of-care molecular diagnostics. Here, we report a multi-channel plasmonic real-time reverse-transcription polymerase chain reaction (mpRT-qPCR) assay for ultrafast and on-chip multi-target detection. The mpRT-qPCR system features two pairs of plasmonic thermocyclers for rapid nanostructure-driven amplification and microlens array fluorescence microscopes for

Identifiants

DOI: 10.1039/d3lc00687e PMID: 37823261
pubmed: 37823261
doi: 10.1039/d3lc00687e
doi:

Substances chimiques

RNA, Viral 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4701-4707

Auteurs

Byoung-Hoon Kang (BH)

Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. kjeong@kaist.ac.kr.
KAIST Institute for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

Kyung-Won Jang (KW)

Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. kjeong@kaist.ac.kr.
KAIST Institute for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

Eun-Sil Yu (ES)

Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. kjeong@kaist.ac.kr.
KAIST Institute for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

Hyejeong Jeong (H)

Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. kjeong@kaist.ac.kr.
KAIST Institute for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

Ki-Hun Jeong (KH)

Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. kjeong@kaist.ac.kr.
KAIST Institute for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
ORCID: 0000-0003-4799-7816

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Single-shot multi-channel plasmonic real-time...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Single-shot multi-channel plasmonic real-time...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN viral › Single-shot multi-channel plasmonic real-time...
questionsmedicales.fr › Administration des services de santé › Gestion des soins aux patients › Systèmes automatisés lit malade › Analyse sur le lieu d'intervention › Single-shot multi-channel plasmonic real-time...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Transcription génétique › Transcription inverse › Single-shot multi-channel plasmonic real-time...
questionsmedicales.fr › Administration des services de santé › Gestion des soins aux patients › Systèmes automatisés lit malade › Single-shot multi-channel plasmonic real-time...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Single-shot multi-channel plasmonic real-time...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Single-shot multi-channel plasmonic real-time...