PTBP2 attenuation facilitates fibroblast to neuron conversion by promoting alternative splicing of neuronal genes.

Humans Alternative Splicing / genetics Neurons / metabolism RNA Splicing Axons / metabolism Fibroblasts / metabolism Nerve Tissue Proteins / genetics Polypyrimidine Tract-Binding Protein / genetics
GABAergic neurons PTBP2 RBFOX3 RNA splicing alternative splicing direct conversion induced neurons nPTB reprogramming transdifferentiation

Journal

Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300

Informations de publication

Date de publication:
14 Nov 2023
Historique:
received: 27 10 2022
revised: 19 09 2023
accepted: 20 09 2023
medline: 17 11 2023
pubmed: 14 10 2023
entrez: 13 10 2023
Statut: ppublish

Résumé

The direct conversion of human skin fibroblasts to neurons has a low efficiency and unclear mechanism. Here, we show that the knockdown of PTBP2 significantly enhanced the transdifferentiation induced by ASCL1, MIR9/9

Identifiants

DOI: 10.1016/j.stemcr.2023.09.012 PMID: 37832540 PMC: PMC10679656
pubmed: 37832540
pii: S2213-6711(23)00371-5
doi: 10.1016/j.stemcr.2023.09.012
pmc: PMC10679656
pii:
doi:

Substances chimiques

PTBP2 protein, human 0
Nerve Tissue Proteins 0
Polypyrimidine Tract-Binding Protein 139076-35-0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2268-2282

Subventions

Organisme : BLRD VA
ID : I01 BX002452
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS113763
Pays : United States

Informations de copyright

Published by Elsevier Inc.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Binglin Zhu (B)

Veterans Affairs Western New York Healthcare System, Buffalo, NY 14215, USA; Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, NY 14203, USA.

Emily Fisher (E)

Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, NY 14203, USA.

Li Li (L)

Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, NY 14203, USA.

Ping Zhong (P)

Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, NY 14203, USA.

Zhen Yan (Z)

Veterans Affairs Western New York Healthcare System, Buffalo, NY 14215, USA; Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, NY 14203, USA.

Jian Feng (J)

Veterans Affairs Western New York Healthcare System, Buffalo, NY 14215, USA; Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, NY 14203, USA. Electronic address: jianfeng@buffalo.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › PTBP2 attenuation facilitates fibroblast to...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Maturation post-transcriptionnelle des ARN › Épissage des ARN › Épissage alternatif › PTBP2 attenuation facilitates fibroblast to...
questionsmedicales.fr › Cellules › Neurones › PTBP2 attenuation facilitates fibroblast to...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Maturation post-transcriptionnelle des ARN › Épissage des ARN › PTBP2 attenuation facilitates fibroblast to...
questionsmedicales.fr › Cellules › Neurones › Neurofibres › Axones › PTBP2 attenuation facilitates fibroblast to...
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Fibroblastes › PTBP2 attenuation facilitates fibroblast to...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de tissu nerveux › PTBP2 attenuation facilitates fibroblast to...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Facteurs d'épissage des ARN › Protéine PTB › PTBP2 attenuation facilitates fibroblast to...