Extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular RNA levels in Plasmodium falciparum.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
13 10 2023
13 10 2023
Historique:
received:
28
10
2022
accepted:
26
09
2023
medline:
23
10
2023
pubmed:
14
10
2023
entrez:
13
10
2023
Statut:
epublish
Résumé
Plasmodium falciparum secretes extracellular vesicles (PfEVs) that contain parasite-derived RNA. However, the significance of the secreted RNA remains unexplored. Here, we compare secreted and intracellular RNA from asexual cultures of six P. falciparum lines. We find that secretion of RNA via extracellular vesicles is not only periodic throughout the asexual intraerythrocytic developmental cycle but is also highly conserved across P. falciparum isolates. We further demonstrate that the phases of RNA secreted via extracellular vesicles are discernibly shifted compared to those of the intracellular RNA within the secreting whole parasite. Finally, transcripts of genes with no known function during the asexual intraerythrocytic developmental cycle are enriched in PfEVs compared to the whole parasite. We conclude that the secretion of extracellular vesicles could be a putative posttranscriptional RNA regulation mechanism that is part of or synergise the classic RNA decay processes to maintain intracellular RNA levels in P. falciparum.
Identifiants
pubmed: 37833314
doi: 10.1038/s41467-023-42103-x
pii: 10.1038/s41467-023-42103-x
pmc: PMC10575976
doi:
Substances chimiques
RNA
63231-63-0
Protozoan Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6447Subventions
Organisme : Wellcome Trust
ID : 222323/Z/21/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 203077/Z/16/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 206194/Z/17/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 209289/Z/17/Z
Pays : United Kingdom
Informations de copyright
© 2023. Springer Nature Limited.
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