Enhanced detection of viruses for improved water safety.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
13 10 2023
Historique:
received: 25 05 2023
accepted: 09 10 2023
medline: 23 10 2023
pubmed: 14 10 2023
entrez: 13 10 2023
Statut: epublish

Résumé

Human viruses pose a significant health risk in freshwater environments, but current monitoring methods are inadequate for detecting viral presence efficiently. We evaluated a novel passive in-situ concentration method using granular activated carbon (GAC). This study detected and quantified eight enteric and non-enteric, pathogenic viruses in a freshwater recreational lake in paired grab and GAC passive samples. The results found that GAC passive sampling had a higher detection rate for all viruses compared to grab samples, with adenovirus found to be the most prevalent virus, followed by respiratory syncytial virus, norovirus, enterovirus, influenza A, SARS-CoV-2, and rotavirus. GAC in-situ concentration allowed for the capture and recovery of viral gene copy targets that ranged from one to three orders of magnitude higher than conventional ex-situ concentration methods used in viral monitoring. This simple and affordable sampling method may have far-reaching implications for reducing barriers associated with viral monitoring across various environmental contexts.

Identifiants

pubmed: 37833399
doi: 10.1038/s41598-023-44528-2
pii: 10.1038/s41598-023-44528-2
pmc: PMC10575868
doi:

Substances chimiques

Water 059QF0KO0R

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

17336

Informations de copyright

© 2023. Springer Nature Limited.

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Auteurs

Emalie K Hayes (EK)

Centre for Water Resources Studies, Department of Civil and Resource Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS, B3H 4R2, Canada. Emalie.hayes@dal.ca.

Madison T Gouthro (MT)

Centre for Water Resources Studies, Department of Civil and Resource Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS, B3H 4R2, Canada.

Megan Fuller (M)

Centre for Water Resources Studies, Department of Civil and Resource Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS, B3H 4R2, Canada.

David J Redden (DJ)

Centre for Water Resources Studies, Department of Civil and Resource Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS, B3H 4R2, Canada.

Graham A Gagnon (GA)

Centre for Water Resources Studies, Department of Civil and Resource Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS, B3H 4R2, Canada. Graham.Gagnon@dal.ca.

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