Acute toxicological evaluation of AT-533 and AT-533 gel in Sprague-Dawley rats.


Journal

BMC pharmacology & toxicology
ISSN: 2050-6511
Titre abrégé: BMC Pharmacol Toxicol
Pays: England
ID NLM: 101590449

Informations de publication

Date de publication:
13 10 2023
Historique:
received: 20 04 2023
accepted: 10 10 2023
medline: 23 10 2023
pubmed: 14 10 2023
entrez: 14 10 2023
Statut: epublish

Résumé

AT-533 is a novel heat shock protein 90 inhibitor that exerting anti-inflammatory, antiviral, and antitumor efficacy. Furthermore, the gel made of AT-533 as raw material named AT-533 gel has the function of repairing keratitis and dermatitis caused by herpes virus infection. However, the acute safety evaluation of AT-533 and AT-533 gel has not been conducted. Herein, we performed acute toxicological studies of AT-533 and AT-533 gel in Sprague-Dawley rats. Fifteen-day acute toxicity study of AT-533 was conducted in both male and female Sprague-Dawley rats at doses of 5, 50, 250 and 500 mg/kg and AT-533 gel at 5 g/kg in the study. During experiment, food consumption and mortality were observed and body weight, hematology, serum biochemistry and histopathological assessment of rats were carried out. No abnormal changes were observed in rats percutaneously treated with AT-533 at 5 mg/kg and 50 mg/kg and AT-533 gel. However, loss of appetite and body weight, adverse reactions, toxicologically relevant alterations in hematology and biochemistry were found in rats percutaneously treated with AT-533 at 250 mg/kg and 500 mg/kg during 15-day acute dermic toxicity study. The aforementioned results suggested that the LD

Sections du résumé

BACKGROUND
AT-533 is a novel heat shock protein 90 inhibitor that exerting anti-inflammatory, antiviral, and antitumor efficacy. Furthermore, the gel made of AT-533 as raw material named AT-533 gel has the function of repairing keratitis and dermatitis caused by herpes virus infection. However, the acute safety evaluation of AT-533 and AT-533 gel has not been conducted.
METHODS AND RESULTS
Herein, we performed acute toxicological studies of AT-533 and AT-533 gel in Sprague-Dawley rats. Fifteen-day acute toxicity study of AT-533 was conducted in both male and female Sprague-Dawley rats at doses of 5, 50, 250 and 500 mg/kg and AT-533 gel at 5 g/kg in the study. During experiment, food consumption and mortality were observed and body weight, hematology, serum biochemistry and histopathological assessment of rats were carried out. No abnormal changes were observed in rats percutaneously treated with AT-533 at 5 mg/kg and 50 mg/kg and AT-533 gel. However, loss of appetite and body weight, adverse reactions, toxicologically relevant alterations in hematology and biochemistry were found in rats percutaneously treated with AT-533 at 250 mg/kg and 500 mg/kg during 15-day acute dermic toxicity study.
CONCLUSIONS
The aforementioned results suggested that the LD

Identifiants

pubmed: 37833798
doi: 10.1186/s40360-023-00696-5
pii: 10.1186/s40360-023-00696-5
pmc: PMC10576390
doi:

Substances chimiques

AT-533 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

54

Informations de copyright

© 2023. BioMed Central Ltd., part of Springer Nature.

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Auteurs

Lishan Zhong (L)

Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China.
Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China.
Guangdong Provincial biotechnology drug and Engineering Technology Research Center, Guangzhou, China.

Yanting Wu (Y)

Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China.
Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China.
Guangdong Provincial biotechnology drug and Engineering Technology Research Center, Guangzhou, China.
Guangzhou (Jinan) Biomedical Research and Development Center Co. Ltd, Guangzhou, China.

Chen Huang (C)

Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China.
Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China.
Guangdong Provincial biotechnology drug and Engineering Technology Research Center, Guangzhou, China.

Kaisheng Liu (K)

Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatrics,Shenzhen People's Hospital, The Second Clinical Medical College, Jinan University, Shenzhen, Guangdong, China. liukaisheng@szhospital.com.
Shenzhen People's Hospital, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, Guangdong, China. liukaisheng@szhospital.com.

Cui-Fang Ye (CF)

Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China.
Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China.
Guangdong Provincial biotechnology drug and Engineering Technology Research Center, Guangzhou, China.

Zhe Ren (Z)

Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China.
Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China.
Guangdong Provincial biotechnology drug and Engineering Technology Research Center, Guangzhou, China.

Yifei Wang (Y)

Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China. twang-yf@163.com.
Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China. twang-yf@163.com.
Guangdong Provincial biotechnology drug and Engineering Technology Research Center, Guangzhou, China. twang-yf@163.com.
Guangzhou (Jinan) Biomedical Research and Development Center Co. Ltd, Guangzhou, China. twang-yf@163.com.

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