Effects of Benzo[a]pyrene on Human Sperm Functions: An In Vitro Study.
Benzo(a)pyrene (BaP)
Polycyclic Aromatic Hydrocarbons (PAHs)
acrosome reaction
apoptosis
human spermatozoa
hyperactivation
reactive oxygen species
sperm DNA fragmentation
sperm motility
spermatozoa able to penetrate in a viscous medium
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
22 Sep 2023
22 Sep 2023
Historique:
received:
31
08
2023
revised:
18
09
2023
accepted:
20
09
2023
medline:
23
10
2023
pubmed:
14
10
2023
entrez:
14
10
2023
Statut:
epublish
Résumé
Benzo(a)pyrene (BaP) is considered one of the most dangerous air pollutants for adverse health effects, including reproductive toxicity. It is found both in male and female reproductive fluids likely affecting spermatozoa after the selection process through cervical mucus, a process mimicked in vitro with the swim-up procedure. In vitro effects of BaP (1, 5, 10 µM) were evaluated both in unselected and swim-up selected spermatozoa after 3 and 24 h of incubation. BaP reduced total, progressive and hyperactivated motility and migration in a viscous medium both in swim-up selected and unselected spermatozoa. Viability was not significantly affected in swim-up selected but was reduced in unselected spermatozoa. In swim-up selected spermatozoa, increases in the percentage of spontaneous acrosome reaction and DNA fragmentation were observed after 24 h of incubation, whereas no differences between the control and BaP-treated samples were observed in caspase-3 and -7 activity, indicating no effects on apoptotic pathways. ROS species, evaluated by staining with CellROX
Identifiants
pubmed: 37833859
pii: ijms241914411
doi: 10.3390/ijms241914411
pmc: PMC10572991
pii:
doi:
Substances chimiques
Benzo(a)pyrene
3417WMA06D
Reactive Oxygen Species
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Italian University and Research
ID : 2017TK7Z8L_006
Références
BMC Public Health. 2021 Sep 16;21(1):1692
pubmed: 34530795
Front Pharmacol. 2016 Aug 08;7:245
pubmed: 27551266
Front Biosci (Landmark Ed). 2014 Jan 01;19(2):291-303
pubmed: 24389185
Biol Reprod. 2012 Nov 08;87(5):110
pubmed: 22933515
Environ Health Perspect. 2002 Jun;110 Suppl 3:451-88
pubmed: 12060843
Asian J Androl. 2020 Sep-Oct;22(5):465-471
pubmed: 31939350
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2018;53(14):1221-1228
pubmed: 30623705
Hum Reprod. 2007 Apr;22(4):961-7
pubmed: 17218370
Nat Rev Endocrinol. 2022 Mar;18(3):139-157
pubmed: 34912078
Reprod Toxicol. 2008 Jan;25(1):100-6
pubmed: 18065195
Mutagenesis. 2010 Jul;25(4):417-25
pubmed: 20488941
Hum Reprod. 2008 May;23(5):1035-43
pubmed: 18326515
Toxicology. 2021 May 30;456:152780
pubmed: 33862174
Hum Reprod. 2002 Jan;17(1):143-9
pubmed: 11756379
Int J Oncol. 1998 Sep;13(3):557-63
pubmed: 9683793
Front Cell Dev Biol. 2023 May 16;11:1117155
pubmed: 37261076
Reproduction. 2019 Sep 1;158(3):281-290
pubmed: 31437814
Mol Hum Reprod. 1998 Feb;4(2):159-65
pubmed: 9542974
Nanomaterials (Basel). 2021 Feb 13;11(2):
pubmed: 33668575
Hum Reprod. 1998 Aug;13(8):2139-46
pubmed: 9756285
Hum Reprod Update. 2023 Mar 1;29(2):157-176
pubmed: 36377604
Life Sci. 2006 Feb 23;78(13):1401-6
pubmed: 16457855
Environ Pollut. 2023 Jan 15;317:120766
pubmed: 36460192
J Hazard Mater. 2017 May 5;329:241-248
pubmed: 28178639
Fertil Steril. 2010 Jul;94(2):595-8
pubmed: 19324334
Arch Environ Occup Health. 2023;78(1):14-27
pubmed: 35357264
Reprod Biol Endocrinol. 2018 Dec 23;16(1):109
pubmed: 30579357
Andrology. 2014 May;2(3):394-401
pubmed: 24700807
Arch Toxicol. 2009 Sep;83(9):873-83
pubmed: 19526226
Asian J Androl. 2015 Jul-Aug;17(4):633-9
pubmed: 25999358
J Biol Chem. 2012 Sep 21;287(39):33048-60
pubmed: 22851170
Reprod Toxicol. 2001 Sep-Oct;15(5):479-86
pubmed: 11780955
Eur J Histochem. 2021 Aug 13;65(s1):
pubmed: 34459573
Int Arch Occup Environ Health. 2006 May;79(5):349-56
pubmed: 16362321
Int J Mol Sci. 2022 Jun 06;23(11):
pubmed: 35683027
Regul Toxicol Pharmacol. 2006 Jul;45(2):159-68
pubmed: 16632147
Mol Med. 2015 Jan 30;21:109-22
pubmed: 25786204
Free Radic Res. 2010 May;44(5):479-96
pubmed: 20370557
Reprod Toxicol. 2011 May;31(4):402-8
pubmed: 21172420
Commun Biol. 2019 Jun 20;2:228
pubmed: 31240266
Hum Exp Toxicol. 2014 Feb;33(2):196-202
pubmed: 23703818
Environ Pollut. 2016 Jan;208(Pt A):110-117
pubmed: 26428471
Mol Med Rep. 2017 May;15(5):3398-3404
pubmed: 28350051
Int J Androl. 1999 Feb;22(1):37-42
pubmed: 10068942
Biol Reprod. 2021 Aug 3;105(2):317-331
pubmed: 34057175
Mol Cell Endocrinol. 2020 Oct 1;516:110952
pubmed: 32712385
Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):4892-6
pubmed: 21383182
Hum Reprod. 1995 Oct;10 Suppl 1:15-21
pubmed: 8592032
Reprod Toxicol. 2003 Sep-Oct;17(5):527-37
pubmed: 14555190
Chemosphere. 2021 Jan;263:128016
pubmed: 33297042
J Clin Med. 2019 Apr 25;8(4):
pubmed: 31027257
Front Endocrinol (Lausanne). 2022 Nov 22;13:1012416
pubmed: 36483001
Environ Pollut. 2016 Jul;214:657-667
pubmed: 27140679
Int J Mol Sci. 2023 Jan 11;24(2):
pubmed: 36674957
Environ Res. 1982 Aug;28(2):410-33
pubmed: 6749489
Hum Reprod. 2014 Mar;29(3):418-28
pubmed: 24430778
Cancer Lett. 1999 Nov 15;146(2):127-34
pubmed: 10656617