miR-124 and VAMP3 Act Antagonistically in Human Neuroblastoma.
VAMP3
apoptosis
gene expression
miR-124
neuroblastoma
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
04 Oct 2023
04 Oct 2023
Historique:
received:
05
09
2023
revised:
25
09
2023
accepted:
02
10
2023
medline:
1
11
2023
pubmed:
14
10
2023
entrez:
14
10
2023
Statut:
epublish
Résumé
Neuroblastoma (NB) is the most common extracranial solid tumor that affects developing nerve cells in the fetus, infants, and children. miR-124 is a microRNA (miRNA) enriched in neuronal tissues, and VAMP3 (vesicle-associated membrane protein 3) has been reported to be an miR-124 target, although the relationship between NB and miR-124 or VAMP3 is unknown. Our current work identified that miR-124 levels are high in NB cases and that elevated miR-124 correlates with worse NB outcomes. Conversely, depressed VAMP3 correlates with worse NB outcomes. To investigate the mechanisms by which miR-124 and VAMP3 regulate NB, we altered miR-124 or VAMP3 expression in human NB cells and observed that increased miR-124 and reduced VAMP3 stimulated cell proliferation and suppressed apoptosis, while increased VAMP3 had the opposite effects. Genome-wide mRNA expression analyses identified gene and pathway changes which might explain the NB cell phenotypes. Together, our studies suggest that miR-124 and VAMP3 could be potential new markers of NB and targets of NB treatments.
Identifiants
pubmed: 37834325
pii: ijms241914877
doi: 10.3390/ijms241914877
pmc: PMC10573497
pii:
doi:
Substances chimiques
Vesicle-Associated Membrane Protein 3
0
MicroRNAs
0
VAMP3 protein, human
0
MIRN124 microRNA, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11691-6
pubmed: 9751727
J Biol Chem. 2012 Oct 26;287(44):37530-9
pubmed: 22936810
Nat Biotechnol. 2014 Sep;32(9):903-14
pubmed: 25150838
Front Oncol. 2022 Feb 04;12:829045
pubmed: 35186763
Clin Genet. 2010 Apr;77(4):306-13
pubmed: 20132241
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
J Cell Sci. 2012 Aug 15;125(Pt 16):3827-39
pubmed: 22573826
Mol Cancer. 2010 Oct 15;9:277
pubmed: 20950435
FEBS Lett. 2011 Nov 16;585(22):3582-6
pubmed: 22024478
Biomedicines. 2021 Jan 19;9(1):
pubmed: 33478086
J Cell Biol. 2000 May 1;149(3):697-706
pubmed: 10791982
Cancer Med. 2019 Sep;8(12):5638-5650
pubmed: 31389160
J Biosci. 2017 Dec;42(4):555-563
pubmed: 29229874
Cell Mol Neurobiol. 2022 Oct;42(7):2031-2053
pubmed: 33886036
Comput Math Methods Med. 2022 May 4;2022:7525048
pubmed: 35572821
Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9779-84
pubmed: 12902540
Mol Cell. 2014 Oct 2;56(1):104-15
pubmed: 25263593
Cancer Res. 2005 Apr 15;65(8):3136-45
pubmed: 15833843
Curr Oncol Rep. 2022 Aug;24(8):1053-1062
pubmed: 35362827
Biochim Biophys Acta Gene Regul Mech. 2017 Dec;1860(12):1179-1188
pubmed: 29109067
J Clin Oncol. 2009 Jan 10;27(2):298-303
pubmed: 19047290
Signal Transduct Target Ther. 2019 Aug 16;4:27
pubmed: 31637007
Nature. 1993 Jul 22;364(6435):346-9
pubmed: 8332193
Cancer Metastasis Rev. 2020 Mar;39(1):275-285
pubmed: 31927719
Nat Commun. 2015 Apr 21;6:6919
pubmed: 25897521
Mol Cell Biol. 2001 Mar;21(5):1573-80
pubmed: 11238894
Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9559-64
pubmed: 8790369
Dis Model Mech. 2021 Apr 1;14(4):
pubmed: 33973623
Curr Opin Genet Dev. 2011 Aug;21(4):511-7
pubmed: 21530229
Cell. 2018 Mar 22;173(1):20-51
pubmed: 29570994
Oncogene. 2004 Aug 5;23(35):5912-22
pubmed: 15195138
Oncogene. 2022 Jan;41(5):622-633
pubmed: 34789839
J Invest Dermatol. 2019 Nov;139(11):2324-2333
pubmed: 31128202
Front Cell Dev Biol. 2020 Nov 20;8:559553
pubmed: 33330445
Nature. 2012 Feb 22;483(7391):589-93
pubmed: 22367537
Cancer Lett. 2014 Dec 28;355(2):210-6
pubmed: 25264923
Cells. 2019 Jul 30;8(8):
pubmed: 31366019
Trends Genet. 2022 Apr;38(4):379-394
pubmed: 34728089
Cell Death Dis. 2013 Apr 11;4:e586
pubmed: 23579273
Mol Cancer Res. 2020 Jan;18(1):68-78
pubmed: 31624087
BMC Cancer. 2014 May 02;14:309
pubmed: 24885481
Cell Tissue Res. 2018 May;372(2):211-221
pubmed: 29445860
J Clin Oncol. 2000 May;18(9):1888-99
pubmed: 10784629