Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensors.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
14 10 2023
Historique:
received: 27 07 2023
accepted: 13 10 2023
medline: 23 10 2023
pubmed: 15 10 2023
entrez: 14 10 2023
Statut: epublish

Résumé

In this work, we present the development and biofunctionalization of a fiber-optic ball-resonator biosensor for the real-time detection of vaccinia poxvirus. We fabricated several ball-tip resonators, functionalized through a silanization process to immobilize two bioreceptors: the monoclonal anti-L1R antibody targeting the L1R protein, and the polyclonal rabbit serum antibodies targeting the whole vaccinia virus (VV) pathogen. Experimental measurements were carried out to detect VV in concentrations from 10

Identifiants

pubmed: 37838808
doi: 10.1038/s41598-023-44926-6
pii: 10.1038/s41598-023-44926-6
pmc: PMC10576743
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

17470

Informations de copyright

© 2023. Springer Nature Limited.

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Auteurs

Aida Rakhimbekova (A)

Department of Electrical and Computer Engineering, School of Engineering and Digital Sciences, Nazarbayev University, 010000, Astana, Kazakhstan.

Baizak Kudaibergenov (B)

Department of Electrical and Computer Engineering, School of Engineering and Digital Sciences, Nazarbayev University, 010000, Astana, Kazakhstan.

Kuanysh Seitkamal (K)

Department of Biomedical Sciences, School of Medicine, Nazarbayev University, 010000, Astana, Kazakhstan.

Aurora Bellone (A)

Department of Electronics and Telecommunications, Politecnico Di Torino, Turin, Italy.

Ayazhan Dauletova (A)

Department of Electrical and Computer Engineering, School of Engineering and Digital Sciences, Nazarbayev University, 010000, Astana, Kazakhstan.

Marzhan Sypabekova (M)

Department of Electrical & Computer Engineering, Baylor University, Waco, TX, USA.

Massimo Olivero (M)

Department of Electronics and Telecommunications, Politecnico Di Torino, Turin, Italy.

Guido Perrone (G)

Department of Electronics and Telecommunications, Politecnico Di Torino, Turin, Italy.

Antonia Radaelli (A)

Department of Medical Biotechnologies and Translational Medicine, Laboratory of Molecular Virology and Recombinant Vaccine Development, University of Milan, Via Vanvitelli 32, Milan, Italy.
Catholic University "Our Lady of Good Counsel", Rr. Dritan Hoxha, Tirana, Albania.

Carlo Zanotto (C)

Department of Medical Biotechnologies and Translational Medicine, Laboratory of Molecular Virology and Recombinant Vaccine Development, University of Milan, Via Vanvitelli 32, Milan, Italy.

Carlo De Giuli Morghen (C)

Catholic University "Our Lady of Good Counsel", Rr. Dritan Hoxha, Tirana, Albania.

Luca Vangelista (L)

Department of Biomedical Sciences, School of Medicine, Nazarbayev University, 010000, Astana, Kazakhstan.
Department of Molecular Medicine, University of Pavia, 27100, Pavia, Italy.

Daniele Tosi (D)

Department of Electrical and Computer Engineering, School of Engineering and Digital Sciences, Nazarbayev University, 010000, Astana, Kazakhstan. daniele.tosi@nu.edu.kz.
Laboratory of Biosensors and Bioinstruments, National Laboratory Astana, 010000, Astana, Kazakhstan. daniele.tosi@nu.edu.kz.

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