Effects of Deslorelin on Testosterone Secretion and Testicular Volume in Male Rhesus Macaques (Macaca mulatta).

Sterility in male NHP has long been achieved through surgical castration or vasectomy. However, these techniques areirreversible, require a surgical procedure, and have potential consequences such as sperm granulomas and long recovery time.Deslorelin is a gonadotropin-releasing hormone agonist that temporarily and reversibly suppresses sex hormone secretion.Our goal in this study was to investigate the effects of deslorelin on testosterone secretion and testicular volume in malerhesus macaques (Macaca mulatta). Male macaques (n = 4) each received two, 4.7-mg deslorelin implants subcutaneously inthe interscapular region. Serum testosterone and testicular volume were then monitored at specific time points until 10 moafter treatment. Testosterone suppression was defined as testosterone levels lower than 0.6 ng/mL for a sustained period ofat least 30 d. After implantation, mean testicular volume was significantly reduced by day 121. Testosterone suppression wasobserved in all subjects. However, the time from implantation to testosterone suppression and duration of suppression varied.Two macaques were hormonally suppressed by day 26 after implantation and remained suppressed for at least 6 mo. Theother 2 macaques were hormonally suppressed by 2 mo after implantation; of these two, one remained suppressed for 70 dayswhile the other was suppressed for at least 245 days. We conclude that deslorelin can safely suppress testosterone secretionin male rhesus macaques, but individual variation in onset and duration of action should be considered when establishingreimplantation time points and potential return to reproductive activity.