Decreased mononuclear cell NR3C1 SKA2 and FKPB5 expression levels among adult survivors of suicide bombing terror attacks in childhood are associated with the development of PTSD.


Journal

Molecular psychiatry
ISSN: 1476-5578
Titre abrégé: Mol Psychiatry
Pays: England
ID NLM: 9607835

Informations de publication

Date de publication:
16 Oct 2023
Historique:
received: 05 03 2023
accepted: 19 09 2023
revised: 01 09 2023
medline: 17 10 2023
pubmed: 17 10 2023
entrez: 16 10 2023
Statut: aheadofprint

Résumé

Life threatening trauma and the development of PTSD during childhood, may each associate with transcriptional perturbation of immune cell glucocorticoid reactivity, yet their separable longer term contributions are less clear. The current study compared resting mononuclear cell gene expression levels of the nuclear receptor, subfamily 3, member 1 (NR3C1) coding the glucocorticoid receptor, its trans-activator spindle and kinetochore-associated protein 2 (SKA2), and its co-chaperon FKBP prolyl isomerase 5 (FKBP5), between a cohort of young adults first seen at the Hadassah Emergency Department (ED) after surviving a suicide bombing terror attack during childhood, and followed longitudinally over the years, and matched healthy controls not exposed to life threatening trauma. While significant reductions in mononuclear cell gene expression levels were observed among young adults for all three transcripts following early trauma exposure, the development of subsequent PTSD beyond trauma exposure, accounted for a small but significant portion of the variance in each of the three transcripts. Long-term perturbation in the expression of immune cell glucocorticoid response transcripts persists among young adults who develop PTSD following life threatening trauma exposure in childhood, denoting chronic dysregulation of immune stress reactivity.

Identifiants

pubmed: 37845495
doi: 10.1038/s41380-023-02278-7
pii: 10.1038/s41380-023-02278-7
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer Nature Limited.

Références

van Bodegom M, Homberg JR, Henckens M. Modulation of the hypothalamic-pituitary-adrenal axis by early life stress exposure. Front Cell Neurosci. 2017;11:87.
pubmed: 28469557 pmcid: 5395581
Danese A, McEwen BS. Adverse childhood experiences, allostasis, allostatic load, and age-related disease. Physiol Behav. 2012;106:29–39.
pubmed: 21888923 doi: 10.1016/j.physbeh.2011.08.019
Cattane N, Vernon AC, Borsini A, Scassellati C, Endres D, Capuron L, et al. European College of Neuropsychopharmacology ImmunoNeuroPsychiatry Thematic Working G. Preclinical animal models of mental illnesses to translate findings from the bench to the bedside: Molecular brain mechanisms and peripheral biomarkers associated to early life stress or immune challenges. Eur Neuropsychopharmacol. 2022;58:55–79.
pubmed: 35235897 doi: 10.1016/j.euroneuro.2022.02.002
Wieck A, Grassi-Oliveira R, Hartmann do Prado C, Teixeira AL, Bauer ME. Neuroimmunoendocrine interactions in post-traumatic stress disorder: focus on long-term implications of childhood maltreatment. Neuroimmunomodulation. 2014;21:145–51.
pubmed: 24557048 doi: 10.1159/000356552
Tursich M, Neufeld RW, Frewen PA, Harricharan S, Kibler JL, Rhind SG, et al. Association of trauma exposure with proinflammatory activity: a transdiagnostic meta-analysis. Transl Psychiatry. 2014;4:e413.
pubmed: 25050993 pmcid: 4119223 doi: 10.1038/tp.2014.56
Baumeister D, Akhtar R, Ciufolini S, Pariante CM, Mondelli V. Childhood trauma and adulthood inflammation: a meta-analysis of peripheral C-reactive protein, interleukin-6 and tumour necrosis factor-alpha. Mol Psychiatry. 2016;21:642–9.
pubmed: 26033244 doi: 10.1038/mp.2015.67
Lin JE, Neylan TC, Epel E, O’Donovan A. Associations of childhood adversity and adulthood trauma with C-reactive protein: a cross-sectional population-based study. Brain Behav Immun. 2016;53:105–12.
pubmed: 26616398 doi: 10.1016/j.bbi.2015.11.015
Peruzzolo TL, Pinto JV, Roza TH, Shintani AO, Anzolin AP, Gnielka V, et al. Inflammatory and oxidative stress markers in post-traumatic stress disorder: a systematic review and meta-analysis. Mol Psychiatry. 2022;27:3150–63.
pubmed: 35477973 doi: 10.1038/s41380-022-01564-0
O’Donnell CJ, Schwartz Longacre L, Cohen BE, Fayad ZA, Gillespie CF, et al. Posttraumatic stress disorder and cardiovascular disease: state of the science, knowledge gaps, and research opportunities. JAMA Cardiol. 2021;6:1207–16.
pubmed: 34259831 doi: 10.1001/jamacardio.2021.2530
Pace TW, Heim CM. A short review on the psychoneuroimmunology of posttraumatic stress disorder: from risk factors to medical comorbidities. Brain Behav Immun. 2011;25:6–13.
pubmed: 20934505 doi: 10.1016/j.bbi.2010.10.003
Shalev A, Benarroch F, Goltser-Dubner T, Canetti L, Saloner C, Roichman A, et al. Long-term immune alterations accompanying chronic posttraumatic stress disorder following exposure to suicide bomb terror incidents during childhood. Neuropsychobiology. 2017;76:130–5.
pubmed: 29949798 doi: 10.1159/000487275
Segman RH, Goltser-Dubner T, Weiner I, Canetti L, Galili-Weisstub E, Milwidsky A, et al. Blood mononuclear cell gene expression signature of postpartum depression. Mol Psychiatry. 2010;15:93–100.
pubmed: 19581911 doi: 10.1038/mp.2009.65
Kalla C, Goltser-Dubner T, Pevzner D, Canetti L, Mirman A, Ben-Yehuda A, et al. Resting mononuclear cell NR3C1 and SKA2 expression levels predict blunted cortisol reactivity to combat training stress among elite army cadets exposed to childhood adversity. Mol Psychiatry. 2021;26:6680–7.
pubmed: 33981010 doi: 10.1038/s41380-021-01107-z
Eraly SA, Nievergelt CM, Maihofer AX, Barkauskas DA, Biswas N, Agorastos A, et al. Assessment of plasma C-reactive protein as a biomarker of posttraumatic stress disorder risk. JAMA Psychiatry. 2014;71:423–31.
pubmed: 24576974 pmcid: 4032578 doi: 10.1001/jamapsychiatry.2013.4374
van Zuiden M, Geuze E, Willemen HL, Vermetten E, Maas M, Amarouchi K, et al. Glucocorticoid receptor pathway components predict posttraumatic stress disorder symptom development: a prospective study. Biol Psychiatry. 2012;71:309–16.
pubmed: 22137507 doi: 10.1016/j.biopsych.2011.10.026
Palma-Gudiel H, Cordova-Palomera A, Leza JC, Fananas L. Glucocorticoid receptor gene (NR3C1) methylation processes as mediators of early adversity in stress-related disorders causality: a critical review. Neurosci Biobehav Rev. 2015;55:520–35.
pubmed: 26073068 doi: 10.1016/j.neubiorev.2015.05.016
Romens SE, McDonald J, Svaren J, Pollak SD. Associations between early life stress and gene methylation in children. Child Dev. 2015;86:303–9.
pubmed: 25056599 doi: 10.1111/cdev.12270
Gola H, Engler A, Morath J, Adenauer H, Elbert T, Kolassa IT, et al. Reduced peripheral expression of the glucocorticoid receptor alpha isoform in individuals with posttraumatic stress disorder: a cumulative effect of trauma burden. PLoS One. 2014;9:e86333.
pubmed: 24466032 pmcid: 3897679 doi: 10.1371/journal.pone.0086333
Su TP, Zhang L, Chung MY, Chen YS, Bi YM, Chou YH, et al. Levels of the potential biomarker p11 in peripheral blood cells distinguish patients with PTSD from those with other major psychiatric disorders. J Psychiatr Res. 2009;43:1078–85.
pubmed: 19380152 doi: 10.1016/j.jpsychires.2009.03.010
Schur RR, Boks MP, Rutten BPF, Daskalakis NP, de Nijs L, van Zuiden M, et al. Longitudinal changes in glucocorticoid receptor exon 1F methylation and psychopathology after military deployment. Transl Psychiatry. 2017;7:e1181.
pubmed: 28742078 pmcid: 5538126 doi: 10.1038/tp.2017.150
Gonzalez Ramirez C, Villavicencio Queijeiro A, Jimenez Morales S, Barcenas Lopez D, Hidalgo Miranda A, Ruiz Chow A, et al. The NR3C1 gene expression is a potential surrogate biomarker for risk and diagnosis of posttraumatic stress disorder. Psychiatry Res. 2020;284:112797.
pubmed: 31982660 doi: 10.1016/j.psychres.2020.112797
Labonte B, Azoulay N, Yerko V, Turecki G, Brunet A. Epigenetic modulation of glucocorticoid receptors in posttraumatic stress disorder. Transl Psychiatry. 2014;4:e368.
pubmed: 24594779 pmcid: 3966043 doi: 10.1038/tp.2014.3
Rice L, Waters CE, Eccles J, Garside H, Sommer P, Kay P, et al. Identification and functional analysis of SKA2 interaction with the glucocorticoid receptor. J Endocrinol 2008;198:499–509.
pubmed: 18583474 pmcid: 2518725 doi: 10.1677/JOE-08-0019
Guintivano J, Brown T, Newcomer A, Jones M, Cox O, Maher BS, et al. Identification and replication of a combined epigenetic and genetic biomarker predicting suicide and suicidal behaviors. Am J Psychiatry. 2014;171:1287–96.
pubmed: 25073599 pmcid: 7081376 doi: 10.1176/appi.ajp.2014.14010008
Boks MP, Rutten BP, Geuze E, Houtepen LC, Vermetten E, Kaminsky Z, et al. SKA2 methylation is involved in cortisol stress reactivity and predicts the development of post-traumatic stress disorder (PTSD) after military deployment. Neuropsychopharmacology. 2016;41:1350–6.
pubmed: 26361058 doi: 10.1038/npp.2015.286
Binder EB. The role of FKBP5, a co-chaperone of the glucocorticoid receptor in the pathogenesis and therapy of affective and anxiety disorders. Psychoneuroendocrinology. 2009;34:S186–95.
pubmed: 19560279 doi: 10.1016/j.psyneuen.2009.05.021
Levy-Gigi E, Szabo C, Kelemen O, Keri S. Association among clinical response, hippocampal volume, and FKBP5 gene expression in individuals with posttraumatic stress disorder receiving cognitive behavioral therapy. Biol Psychiatry. 2013;74:793–800.
pubmed: 23856297 doi: 10.1016/j.biopsych.2013.05.017
Yehuda R, Cai G, Golier JA, Sarapas C, Galea S, Ising M, et al. Gene expression patterns associated with posttraumatic stress disorder following exposure to the World Trade Center attacks. Biol Psychiatry. 2009;66:708–11.
pubmed: 19393990 doi: 10.1016/j.biopsych.2009.02.034
Sarapas C, Cai G, Bierer LM, Golier JA, Galea S, Ising M, et al. Genetic markers for PTSD risk and resilience among survivors of the World Trade Center attacks. Dis Markers. 2011;30:101–10.
pubmed: 21508514 pmcid: 3825240 doi: 10.1155/2011/328054
Szabo C, Kelemen O, Keri S. Changes in FKBP5 expression and memory functions during cognitive-behavioral therapy in posttraumatic stress disorder: a preliminary study. Neurosci Lett. 2014;569:116–20.
pubmed: 24704382 doi: 10.1016/j.neulet.2014.03.059
Kuan PF, Waszczuk MA, Kotov R, Clouston S, Yang X, Singh PK, et al. Gene expression associated with PTSD in World Trade Center responders: An RNA sequencing study. Transl Psychiatry. 2017;7:1297.
pubmed: 29249826 pmcid: 5802695 doi: 10.1038/s41398-017-0050-1
Kuan PF, Yang X, Clouston S, Ren X, Kotov R, Waszczuk M, et al. Cell type-specific gene expression patterns associated with posttraumatic stress disorder in World Trade Center responders. Transl Psychiatry. 2019;9:1.
pubmed: 30664621 pmcid: 6341096 doi: 10.1038/s41398-018-0355-8
Klengel T, Mehta D, Anacker C, Rex-Haffner M, Pruessner JC, Pariante CM, et al. Allele-specific FKBP5 DNA demethylation mediates gene-childhood trauma interactions. Nat Neurosci. 2013;16:33–41.
pubmed: 23201972 doi: 10.1038/nn.3275
Yin H, Galfalvy H, Pantazatos SP, Huang YY, Rosoklija GB, Dwork AJ, et al. Glucocorticoid receptor-related genes: genotype and brain gene expression relationships to suicide and major depressive disorder. Depress Anxiety. 2016;33:531–40.
pubmed: 27030168 pmcid: 4889464 doi: 10.1002/da.22499
Watkeys OJ, Kremerskothen K, Quide Y, Fullerton JM, Green MJ. Glucocorticoid receptor gene (NR3C1) DNA methylation in association with trauma, psychopathology, transcript expression, or genotypic variation: a systematic review. Neurosci Biobehav Rev. 2018;95:85–122.
pubmed: 30176278 doi: 10.1016/j.neubiorev.2018.08.017
Katrinli S, Oliveira NCS, Felger JC, Michopoulos V, Smith AK. The role of the immune system in posttraumatic stress disorder. Transl Psychiatry. 2022;12:313.
pubmed: 35927237 pmcid: 9352784 doi: 10.1038/s41398-022-02094-7
DePierro J, Lepow L, Feder A, Yehuda R. Translating molecular and neuroendocrine findings in posttraumatic stress disorder and resilience to novel therapies. Biol Psychiatry. 2019;86:454–63.
pubmed: 31466562 pmcid: 6907400 doi: 10.1016/j.biopsych.2019.07.009
Passos IC, Vasconcelos-Moreno MP, Costa LG, Kunz M, Brietzke E, Quevedo J, et al. Inflammatory markers in post-traumatic stress disorder: a systematic review, meta-analysis, and meta-regression. Lancet Psychiatry. 2015;2:1002–12.
pubmed: 26544749 doi: 10.1016/S2215-0366(15)00309-0
Hori H, Kim Y. Inflammation and post-traumatic stress disorder. Psychiatry Clin Neurosci. 2019;73:143–53.
pubmed: 30653780 doi: 10.1111/pcn.12820
Gupta S, Guleria RS. Involvement of nuclear factor-kappaB in inflammation and neuronal plasticity associated with post-traumatic stress disorder. Cells. 2022;11:2034.
pubmed: 35805118 pmcid: 9265339 doi: 10.3390/cells11132034
Edmondson D, von Kanel R. Post-traumatic stress disorder and cardiovascular disease. Lancet Psychiatry. 2017;4:320–9.
pubmed: 28109646 pmcid: 5499153 doi: 10.1016/S2215-0366(16)30377-7
Boscarino JA. A prospective study of PTSD and early-age heart disease mortality among Vietnam veterans: implications for surveillance and prevention. Psychosom Med. 2008;70:668–76.
pubmed: 18596248 pmcid: 3552245 doi: 10.1097/PSY.0b013e31817bccaf
Daskalakis NP, Cohen H, Cai G, Buxbaum JD, Yehuda R. Expression profiling associates blood and brain glucocorticoid receptor signaling with trauma-related individual differences in both sexes. Proc Natl Acad Sci USA. 2014;111:13529–34.
pubmed: 25114262 pmcid: 4169965 doi: 10.1073/pnas.1401660111
Zaba M, Kirmeier T, Ionescu IA, Wollweber B, Buell DR, Gall-Kleebach DJ, et al. Identification and characterization of HPA-axis reactivity endophenotypes in a cohort of female PTSD patients. Psychoneuroendocrinology. 2015;55:102–15.
pubmed: 25745955 doi: 10.1016/j.psyneuen.2015.02.005
Gadek-Michalska A, Tadeusz J, Rachwalska P, Bugajski J. Cytokines, prostaglandins and nitric oxide in the regulation of stress-response systems. Pharm Rep. 2013;65:1655–62.
doi: 10.1016/S1734-1140(13)71527-5
Menard C, Pfau ML, Hodes GE, Kana V, Wang VX, Bouchard S, et al. Social stress induces neurovascular pathology promoting depression. Nat Neurosci. 2017;20:1752–60.
pubmed: 29184215 pmcid: 5726568 doi: 10.1038/s41593-017-0010-3
Borsini A, Zunszain PA, Thuret S, Pariante CM. The role of inflammatory cytokines as key modulators of neurogenesis. Trends Neurosci. 2015;38:145–57.
pubmed: 25579391 doi: 10.1016/j.tins.2014.12.006
Van Moortel L, Gevaert K, De Bosscher K. Improved glucocorticoid receptor ligands: fantastic beasts, but how to find them? Front Endocrinol (Lausanne). 2020;11:559673.
pubmed: 33071974 doi: 10.3389/fendo.2020.559673
Florido A, Velasco ER, Monari S, Cano M, Cardoner N, Sandi C, et al. Glucocorticoid-based pharmacotherapies preventing PTSD. Neuropharmacology. 2023;224:109344.
pubmed: 36402246 doi: 10.1016/j.neuropharm.2022.109344
Dunlop BW, Wong A. The hypothalamic-pituitary-adrenal axis in PTSD: pathophysiology and treatment interventions. Prog Neuropsychopharmacol Biol Psychiatry. 2019;89:361–79.
pubmed: 30342071 doi: 10.1016/j.pnpbp.2018.10.010

Auteurs

Tanya Goltser-Dubner (T)

Molecular Psychiatry Laboratory, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.
The Herman-Danna Division of Pediatric Psychiatry, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Amit Shalev (A)

The Herman-Danna Division of Pediatric Psychiatry, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Fortu Benarroch (F)

The Herman-Danna Division of Pediatric Psychiatry, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Laura Canetti (L)

Department of Psychology, Hebrew University, Jerusalem, Israel.

Maayan Yogev (M)

Molecular Psychiatry Laboratory, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Carmel Kalla (C)

Molecular Psychiatry Laboratory, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Ranin Masarwa (R)

The Herman-Danna Division of Pediatric Psychiatry, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Josef Martin (J)

The Herman-Danna Division of Pediatric Psychiatry, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Dalya Pevzner (D)

Molecular Psychiatry Laboratory, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Osnat Oz (O)

Molecular Psychiatry Laboratory, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Chen Saloner (C)

Molecular Psychiatry Laboratory, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Reaan Amer (R)

Molecular Psychiatry Laboratory, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Michal Lavon (M)

Molecular Psychiatry Laboratory, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Amit Lotan (A)

Molecular Psychiatry Laboratory, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Esti Galili-Weisstub (E)

The Herman-Danna Division of Pediatric Psychiatry, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel.

Ronen Segman (R)

Molecular Psychiatry Laboratory, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel. ronense@ekmd.huji.ac.il.
The Herman-Danna Division of Pediatric Psychiatry, Department of Psychiatry, Hadassah Medical Organization and Faculty of Medicine, Hebrew University, Jerusalem, Israel. ronense@ekmd.huji.ac.il.

Classifications MeSH