Effect of Host Cholesterol on the Membrane Dynamics of Outer Membrane Lipids of Mycobacteria.

Cholesterol Latent Tubercular infection Membrane order Membrane-drug interactions Mycobacterial membrane lipids Phase-segregated lipid domains

Journal

Chemistry, an Asian journal
ISSN: 1861-471X
Titre abrégé: Chem Asian J
Pays: Germany
ID NLM: 101294643

Informations de publication

Date de publication:
01 Dec 2023
Historique:
revised: 16 10 2023
received: 09 08 2023
medline: 4 12 2023
pubmed: 17 10 2023
entrez: 17 10 2023
Statut: ppublish

Résumé

The ability of Mycobacterium tuberculosis to remain dormant after primary infection represents the prime cause of new TB cases throughout the world. Hence, diagnosis and treatment of individuals hosting dormant mycobacterium is one of the crucial strategies to be adopted for the prevention of Tuberculosis. Among many strategies unleashed by the latent bacterium, one of them is scavenging host cholesterol for carbon source. Cholesterol modifies lipid membranes over many scales and here, its effect on mycobacterial membrane biophysics and the subsequent effect on partitioning of antibiotics into cholesterol- enriched mycobacterial membranes was investigated. Our research showed that cholesterol alters the phase state behavior of mycobacterial outer membrane lipids by enhancing the overall membrane order at the headgroup and acyl chain region and is integrated into both ordered and disordered domains/phases, with a preference for the latter. Exogenous cholesterol further alters the drug partitioning behavior of structurally different drugs, pointing to a larger clinical potential of using more hydrophobic medications to target dormant bacteria.

Identifiants

pubmed: 37846643
doi: 10.1002/asia.202300697
doi:

Substances chimiques

Membrane Lipids 0
Cholesterol 97C5T2UQ7J
Lipid Bilayers 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202300697

Subventions

Organisme : DBT (Department of Biotechnology, Government of India
Organisme : IRCC (IIT Bombay

Informations de copyright

© 2023 Wiley-VCH GmbH.

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Auteurs

Pranav Adhyapak (P)

Department of Chemistry, Indian Institute of Technology Bombay, Mumbai, 400076, India.

Kuan Liang (K)

National Centre for Magnetic Resonance in Wuhan, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, 430071, Hubei, China.

Mojie Duan (M)

National Centre for Magnetic Resonance in Wuhan, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, 430071, Hubei, China.

Shobhna Kapoor (S)

Department of Chemistry, Indian Institute of Technology Bombay, Mumbai, 400076, India.

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