Heterologous prime-boost immunization induces protection against dengue virus infection in cynomolgus macaques.


Journal

Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724

Informations de publication

Date de publication:
30 Nov 2023
Historique:
medline: 1 12 2023
pubmed: 17 10 2023
entrez: 17 10 2023
Statut: ppublish

Résumé

Currently licensed dengue vaccines do not induce long-term protection in children without previous exposure to dengue viruses in nature. These vaccines are based on selected attenuated strains of the four dengue serotypes and employed in combination for two or three consecutive doses. In our search for a better dengue vaccine candidate, live attenuated strains were followed by non-infectious virus-like particles or the plasmids that generate these particles upon injection into the body. This heterologous prime-boost immunization induced elevated levels of virus-specific antibodies and helped to prevent dengue virus infection in a high proportion of vaccinated macaques. In macaques that remained susceptible to dengue virus, distinct mechanisms were found to account for the immunization failures, providing a better understanding of vaccine actions. Additional studies in humans in the future may help to establish whether this combination approach represents a more effective means of preventing dengue by vaccination.

Identifiants

pubmed: 37846984
doi: 10.1128/jvi.00963-23
pmc: PMC10688363
doi:

Substances chimiques

Antibodies, Viral 0
Dengue Vaccines 0
Vaccines, Virus-Like Particle 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0096323

Subventions

Organisme : Wellcome Trust
ID : 203224/Z/16/Z
Pays : United Kingdom

Déclaration de conflit d'intérêts

N.S., P.K., N.C., and M.S.-L. were listed as inventors in patents and patent applications involving the mature virus-like particles of flaviviruses. Other authors declare that they do not have conflicts of interest.

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Auteurs

Poonsook Keelapang (P)

Department of Microbiology, Faculty of Medicine, Chiang Mai University , Chiang Mai, Thailand.

Chutitorn Ketloy (C)

Center of Excellence in Vaccine Research and Development, Faculty of Medicine, Chulalongkorn University , Bangkok, Thailand.
Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University , Bangkok, Thailand.

Chunya Puttikhunt (C)

Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency , Pathumthani, Thailand.
Division of Dengue Hemorrhagic Fever Research, Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok, Thailand.

Rungtawan Sriburi (R)

Department of Microbiology, Faculty of Medicine, Chiang Mai University , Chiang Mai, Thailand.

Eakachai Prompetchara (E)

Center of Excellence in Vaccine Research and Development, Faculty of Medicine, Chulalongkorn University , Bangkok, Thailand.
Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University , Bangkok, Thailand.

Malinee Sae-Lim (M)

Department of Microbiology, Faculty of Medicine, Chiang Mai University , Chiang Mai, Thailand.

Bunpote Siridechadilok (B)

Division of Dengue Hemorrhagic Fever Research, Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok, Thailand.
Frontier Biodesign and Bioengineering Research Team, National Center for Genetic Engineering and Biotechnology , Pathumthani, Thailand.

Thaneeya Duangchinda (T)

Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency , Pathumthani, Thailand.
Division of Dengue Hemorrhagic Fever Research, Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok, Thailand.

Sansanee Noisakran (S)

Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency , Pathumthani, Thailand.
Division of Dengue Hemorrhagic Fever Research, Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok, Thailand.

Nicha Charoensri (N)

Center for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University , Khon Kaen, Thailand.

Prapat Suriyaphol (P)

Siriraj Informatics and Data Innovation Center, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok, Thailand.

Piyanan Suparattanagool (P)

Clinical Epidemiology Unit, Faculty of Medicine, Khon Kaen University , Khon Kaen, Thailand.

Utaiwan Utaipat (U)

Research Institute for Health Sciences, Chiang Mai University , Chiang Mai, Thailand.

Promsin Masrinoul (P)

Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University at Salaya , Nakhon Pathom, Thailand.

Panisadee Avirutnan (P)

Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency , Pathumthani, Thailand.
Division of Dengue Hemorrhagic Fever Research, Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok, Thailand.

Juthathip Mongkolsapaya (J)

Division of Dengue Hemorrhagic Fever Research, Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok, Thailand.
Wellcome Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford , Oxford, United Kingdom.

Gavin Screaton (G)

Wellcome Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford , Oxford, United Kingdom.

Prasert Auewarakul (P)

Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok, Thailand.

Suchinda Malaivijitnond (S)

National Primate Research Center of Thailand, Chulalongkorn University , Bangkok, Thailand.

Sutee Yoksan (S)

Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University at Salaya , Nakhon Pathom, Thailand.

Prida Malasit (P)

Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency , Pathumthani, Thailand.
Division of Dengue Hemorrhagic Fever Research, Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok, Thailand.

Kiat Ruxrungtham (K)

Center of Excellence in Vaccine Research and Development, Faculty of Medicine, Chulalongkorn University , Bangkok, Thailand.

Rojjanaporn Pulmanausahakul (R)

Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy , Bangkok, Thailand.

Nopporn Sittisombut (N)

Department of Microbiology, Faculty of Medicine, Chiang Mai University , Chiang Mai, Thailand.

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