A method for extraction of exosomes from breast tumour cells and characterisation by transmission electron microscopy.
breast tumour cell
exosome
transmission electron microscopy
Journal
Journal of microscopy
ISSN: 1365-2818
Titre abrégé: J Microsc
Pays: England
ID NLM: 0204522
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
revised:
27
09
2023
received:
29
03
2023
accepted:
02
10
2023
medline:
27
11
2023
pubmed:
19
10
2023
entrez:
19
10
2023
Statut:
ppublish
Résumé
Exosomes can not only be used as markers of tumour metastasis but also be used for noninvasive diagnosis of clinical diseases, which holds significant medical research value. Observing the morphology and distribution of exosomes using transmission electron microscopy (TEM) is highly significant. In this study, we use breast tumour cell (MDA-MB-231) exosomes as a representative sample and focus on the extraction and purification of exosomes, as well as the investigation of optimal conditions for the observation of exosomes using TEM. Through comparative tests, we obtained the optimal dilution concentration and staining method for TEM of exosomes, the best dilution concentration is 100 times, the best negative staining time is 1.5 min. Western blotting and scanning electron microscopy (SEM) confirmed the presence of exosome. The particle size experiment shows that the size of exosomes is mainly distributed between 60 nm and 150 nm. This method provides a reference for TEM sample preparation of subcellular structures and small molecular biomaterials other than exosomes.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
117-122Subventions
Organisme : Natural Science Foundation of Shandong Province
ID : ZR2020MC064
Informations de copyright
© 2023 Royal Microscopical Society.
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