Repair effect of Centella asiatica (L.) extract on damaged HaCaT cells studied by atomic force microscopy.
Centella asiatica (L.) extract
HaCaT cells
atomic force microscopy
damage repair
Journal
Journal of microscopy
ISSN: 1365-2818
Titre abrégé: J Microsc
Pays: England
ID NLM: 0204522
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
revised:
01
10
2023
received:
29
06
2023
accepted:
17
10
2023
medline:
27
11
2023
pubmed:
19
10
2023
entrez:
19
10
2023
Statut:
ppublish
Résumé
People's choice of cosmetics is no longer just 'Follow the trend', but pays more attention to the ingredients of cosmetics, whether the ingredients of cosmetics are beneficial to people's skin health; therefore, more and more skin-healthy ingredients have been discovered and used in cosmetics. In this work, atomic force microscope (AFM) is used to provide physical information about biomolecules and living cells; it brings us a new method of high-precision physical measurement. Centella asiatica (L.) extract has the ability to promote skin wound healing, but its healing effect on damaged HaCaT cells needs to be investigated, which plays a key role in judging the effectiveness of skincare ingredients. The objective of this study was to explore the impact of Centella asiatica (L.) extract on ethanol-damaged human immortalised epidermal HaCaT cells based on AFM. We established a model of cellular damage and evaluated cell viability using the MTT assay. The physical changes of cell height, roughness, adhesion and Young's modulus were measured by AFM. The findings indicated that the Centella asiatica (L.) extract had a good repair effect on injured HaCaT cells, and the optimal concentration was 75 μg/mL.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
148-157Subventions
Organisme : National Natural Science Foundation Program of China
ID : 62175020
Organisme : H2020 Europe Program
ID : 101086226
Organisme : H2020 Europe Program
ID : 734174
Organisme : Jilin Provincial Science and Technology Program
ID : 20220201098GX
Organisme : Jilin Provincial Science and Technology Program
ID : 20210101038JC
Organisme : Jilin Provincial Science and Technology Program
ID : 2020C022-1
Organisme : Jilin Provincial Science and Technology Program
ID : 20190702002GH
Organisme : Jilin Provincial Science and Technology Program
ID : 20190201287JC
Organisme : '111' Project of China
ID : D17017
Organisme : China Scholarship Council
ID : 202107585026
Informations de copyright
© 2023 The Authors. Journal of Microscopy published by John Wiley & Sons Ltd on behalf of Royal Microscopical Society.
Références
Woodby, B., Penta, K., Pecorelli, A., Lila, M. A., & Valacchi, G. (2020). Skin health from the inside out. Annual Review of Food Science and Technology, 11, 235-254.
Fam, V. W., Charoenwoodhipong, P., Sivamani, R. K., Holt, R. R., Keen, C. L., & Hackman, R. M. (2022). Plant-based foods for skin health: A narrative review. Journal of the Academy of Nutrition and Dietetics, 122(3), 614-629.
Chandrika, U. G., & Kumara, P. (2015). Gotu kola (Centella asiatica): Nutritional properties and plausible health benefits. Advances in Food and Nutrition Research, 76, 125-157.
Kirtikar, K. R., & Basu, B. D. (1975). Indian medicinal plants (pp. 2327-2328), Reprint Edition. Bishen Singh Mahendra Pal Singh, Dehradun.
Ghani, A. (1998). Medicinal plants of Bangladesh: Chemical constituents and uses. Asiatic Society of Bangladesh.
Kapoor, L. D. (2000). CRC Handbook of Ayurvedic Medicinal Plants (pp. 117). CRC Press. Inc.
Zainol, M. K., Abd-Hamid, A., Yusof, S., & Muse, R. (2003). Antioxidative activity and total phenolic compounds of leaf, root and petiole of four accessions of Centella asiatica (L.) Urban. Food Chemistry, 81(4), 575-581.
Mustafa, R. A., Hamid, A. A., Mohamed, S., & Bakar, F. A. (2010). Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants. Journal of Food Science, 75(1), C28-C35.
Pittella, F., Dutra, R., Junior, D., Lopes, M. T., & Barbosa, N. (2009). Antioxidant and cytotoxic activities of Centella asiatica (L.) (L)Urb. International Journal of Molecular Sciences, 10(9), 3713-3721.
Park, J., Choi, J., Son, D., Park, E., Song, M., Hellström, M., & Hong, J. (2017). Anti-inflammatory effect of titrated extract of Centella asiatica (L.) in phthalic anhydride-induced allergic dermatitis animal model. International Journal of Molecular Sciences, 18(4), 738.
Puttarak, P., Dilokthornsakul, P., Saokaew, S., Dhippayom, T., Kongkaew, C., Sruamsiri, R., Chuthaputti, A., & Chaiyakunapruk, N. (2017). Effects of Centella asiatica (L.) (L.) Urb. on cognitive function and mood related outcomes: A Systematic Review and Meta-analysis. Scientific Reports, 7(1), 10646.
Torbati, F. A., Ramezani, M., Dehghan, R., Amiri, M. S., Moghadam, A. T., Shakour, N., Elyasi, S., Sahebkar, A., & Emami, S. A. (2021). Ethnobotany, phytochemistry and pharmacological features of Centella asiatica: A comprehensive review. Pharmacological Properties of Plant-Derived Natural Products and Implications for Human Health, 1308, 451-499.
Hashim, P., Sidek, H., Helan, M., Sabery, A., Palanisamy, U. D., & Ilham, M. (2011). Triterpene composition and bioactivities of Centella asiatica. Molecules, 16(2), 1310-1322.
Bylka, W., Znajdek-Awiżeń, P., Studzińska-Sroka, E., Dańczak-Pazdrowska, A., & Brzezińska, M. (2014). Centella asiatica (L.) in dermatology: An overview. Phytotherapy Research, 28(8), 1117-1124.
An, I.-S., An, S., Choe, T.-B., Kang, S.-M., Lee, J. H., Park, I.-C., Jin, Y.-W., Lee, S.-J., & Bae, S. (2012). Centella asiatica (L.) protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes. International Journal of Molecular Medicine, 30(6), 1349-1356.
Jung, E., Lee, J.-A., Shin, S., Roh, K.-B., Kim, J.-H., & Park, D. (2013). Madecassoside inhibits melanin synthesis by blocking ultraviolet-induced inflammation. Molecules, 18(12), 15724-15736.
Kwon, K. J., Bae, S., Kim, K., An, I. S., Ahn, K. J., An, S., & Cha, H. J. (2014). Asiaticoside, a component of Centella asiatica, inhibits melanogenesis in B16F10 mouse melanoma. Molecular Medicine Reports, 10(1), 503-507.
Lee, J.-H., Kim, H.-L., Lee, M. H., You, K. E., Kwon, B.-J., Seo, H. J., & Park, J.-C. (2012). Asiaticoside enhances normal human skin cell migration, attachment and growth in vitro wound healing model. Phytomedicine, 19(13), 1223-1227.
Yamaguchi, Y., & Yoshikawa, K. (2001). Cutaneous wound healing: An update. The Journal of Dermatology, 28(10), 521-534.
Raja, R. (2007). Wound re-epithelialization: Modulating keratinocyte migration in wound healing. Frontiers in Bioscience-Landmark, 12(8), 2849-2868.
Ansardamavandi, A., Tafazzoli-Shadpour, M., Omidvar, R., & Jahanzad, I. (2016). Quantification of effects of cancer on elastic properties of breast tissue by atomic force microscopy. Journal of The Mechanical Behavior of Biomedical Materials, 60, 234-242.
Ditscherlein, L., Jolan Gulden, S., Müller, S., Baumann, R.-P., Peuker, U. A., & Nirschl, H. (2018). Measuring interactions between yeast cells and a micro-sized air bubble via atomic force microscopy. Journal of Colloid and Interface Science, 532, 689-699.
Rashidi, N., Omidvar, R., Tafazzoli-shadpour, M., & Ebrahimi, M. (2014). Evaluation of elastic properties of breast cancer stem-like/tumor initiating cells using atomic force microscopy. 2014 21th Iranian Conference on Biomedical Engineering (ICBME), pp. 134-137. IEEE.
D Antonio, P., Lasalvia, M., Perna, G., & Capozzi, V. (2012). Scale-independent roughness value of cell membranes studied by means of AFM technique. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1818(12), 3141-3148.
Babahosseini, H., Carmichael, B., Strobl, J. S., Mahmoodi, S. N., & Agah, M. (2015). Sub-cellular force microscopy in single normal and cancer cells. Biochemical and Biophysical Research Communications, 463(4), 587-592.
Lazar, P., Zhang, S., Šafářová, K., Li, Q., Froning, J. P., Granatier, J., Hobza, P., Zbořil, R., Besenbacher, F., Dong, M., & Otyepka, M. (2013). Quantification of the interaction forces between metals and graphene by quantum chemical calculations and dynamic force measurements under ambient conditions. ACS Nano, 7(2), 1646-1651.
Wang, S., Tian, X.-F., & Wang, L.-M. (2007). Growth inhibition effect of DL-lysine acetylalicylate on sw480 colon carcinoma cells. Chinese Journal of Cancer Research, 19, 18-21.
Malek-Zietek, K. E., Targosz-Korecka, M., & Szymonski, M. (2017). The impact of hyperglycemia on adhesion between endothelial and cancer cells revealed by single-cell force spectroscopy. Journal of Molecular Recognition, 30(9), e2628.
Jiang, X., Ma, K., Hu, C., Gao, M., Zhang, J., Wang, Y., Chen, Y., Song, Z., & Wang, Z. (2019). Evaluation of 5-fluorouracil-treated lung cancer cells by atomic force microscopy. Analytical Methods, 11(39), 4977-4982.
Beaussart, A., El-Kirat-Chatel, S., Sullan, R. M. A., Alsteens, D., Herman, P., Derclaye, S., & Dufrêne, Y. F. (2014). Quantifying the forces guiding microbial cell adhesion using single-cell force spectroscopy. Nature Protocols, 9(5), 1049-1055.
He, J. D., Luo, H. L., Li, J., Feng, W. T., & Chen, L. B. (2012). Influences of the interferon induced transmembrane protein I on the proliferation, invasion, and metastasis of the colorectal cancer SW480 cell lines. Chinese Medical Journal, 125(03), 517-522.
Wang, T., Liu, Y., & Wu, C. (2017). Effect of paclitaxel-mesoporous silica nanoparticles with a core-shell structure on the human lung cancer cell line A549. Nanoscale Research Letters, 12, 1-8.
Li, M., Liu, L., Xi, N., Wang, Y., Xiao, X., & Zhang, W. (2015). Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy. Science China Life Sciences, 58, 889-901.
Garcia, R. (2020). Nanomechanical mapping of soft materials with the atomic force microscope: Methods, theory and applications. Chemical Society Reviews, 49(16), 5850-5884.
Garcia, P. D., Guerrero, C. R., & Garcia, R. (2017). Time-resolved nanomechanics of a single cell under the depolymerization of the cytoskeleton. Nanoscale, 9(33), 12051-12059.
Zhou, G., Zhang, B., Tang, G., Yu, X.-F., & Galluzzi, M. (2021). Cells nanomechanics by atomic force microscopy: Focus on interactions at nanoscale. Advances in Physics: X, 6(1), 1866668.
Li, M., Xi, N., Wang, Y., & Liu, L. (2019). Advances in atomic force microscopy for single-cell analysis. Nano Research, 12, 703-718.
Li, Q. S., Lee, G. Y. H., Ong, C. N., & Lim, C. T. (2008). AFM indentation study of breast cancer cells. Biochemical and Biophysical Research Communications, 374(4), 609-613.
Pellegrin, S., & Mellor, H. (2007). Actin stress fibres. Journal of Cell Science, 120(20), 3491-3499.
Birukova, A. A., Arce, F. T., Moldobaeva, N., Dudek, S. M., Garcia, J. G. N., Lal, R., & Birukov, K. G. (2009). Endothelial permeability is controlled by spatially defined cytoskeletal mechanics: Atomic force microscopy force mapping of pulmonary endothelial monolayer. Nanomedicine: Nanotechnology, Biology and Medicine, 5(1), 30-41.
Chouinard, J. A., Grenier, G., Khalil, A., & Vermette, P. (2008). Oxidized-LDL induce morphological changes and increase stiffness of endothelial cells. Experimental Cell Research, 314(16), 3007-3016.
Rotsch, C., & Radmacher, M. (2000). Drug-induced changes of cytoskeletal structure and mechanics in fibroblasts: An atomic force microscopy study. Biophysical Journal, 78(1), 520-535.
Proksch, E., Brandner, J. M., & Jensen, J.-M. (2008). The skin: An indispensable barrier. Experimental dermatology, 17(12), 1063-1072.
Hsieh, C.-H., Lin, Y.-H., Lin, S., Tsai-Wu, J.-J., Herbert Wu, C. H., & Jiang, C.-C. (2008). Surface ultrastructure and mechanical property of human chondrocyte revealed by atomic force microscopy. Osteoarthritis and Cartilage, 16(4), 480-488.
Suresh, S., Spatz, J., Mills, J. P., Micoulet, A., Dao, M., Lim, C. T., Beil, M., & Seufferlein, T. (2005). Connections between single-cell biomechanics and human disease states: Gastrointestinal cancer and malaria. Acta Biomaterialia, 1(1), 15-30.
Lieber, S. C., Aubry, N., Pain, J., Diaz, G., Kim, S.-J., & Vatner, S. F. (2004). Aging increases stiffness of cardiac myocytes measured by atomic force microscopy nanoindentation. American Journal of Physiology-Heart and Circulatory Physiology, 287(2), H645-H651.
Starodubtseva, M. N. (2011). Mechanical properties of cells and ageing. Ageing Research Reviews, 10(1), 16-25.