Strategies for quantifying the enzymatic activities of glycoside hydrolases within cells and in vivo.
Aggregation-induced emission
Bioluminescence imaging
Cell imaging
Enzyme kinetics
Fluorescence imaging
Fluorescence quenching
Glycosidase
Glycoside hydrolase
High content imaging
Photoacoustic imaging
Ratiometric substrate
Substrate synthesis
Journal
Current opinion in chemical biology
ISSN: 1879-0402
Titre abrégé: Curr Opin Chem Biol
Pays: England
ID NLM: 9811312
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
11
03
2023
revised:
21
09
2023
accepted:
22
09
2023
medline:
1
12
2023
pubmed:
20
10
2023
entrez:
19
10
2023
Statut:
ppublish
Résumé
Within their native milieu of the cell, the activities of enzymes are controlled by a range of factors including protein interactions and post-translational modifications. The involvement of these factors in fundamental cell biology and the etiology of diseases is stimulating interest in monitoring enzyme activities within tissues. The creation of synthetic substrates, and their use with different imaging modalities, to detect and quantify enzyme activities has great potential to propel these areas of research. Here we describe the latest developments relating to the creation of substrates for imaging and quantifying the activities of glycoside hydrolases, focusing on mammalian systems. The limitations of current tools and the difficulties within the field are summarised, as are prospects for overcoming these challenges.
Identifiants
pubmed: 37856901
pii: S1367-5931(23)00141-2
doi: 10.1016/j.cbpa.2023.102403
pii:
doi:
Substances chimiques
Glycoside Hydrolases
EC 3.2.1.-
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
102403Informations de copyright
Copyright © 2023 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: David Vocadlo reports a relationship with Alectos Therapeutics that includes: board membership, consulting and advisory, and equity. David Vocadlo is an inventor of a pending patent on Substrates for imaging glucocerebrosidase activity assigned to Simon Fraser University. Matthew Deen is an inventor of a pending patent on Substrates for imaging glucocerebrosidase activity assigned to Simon Fraser University.