Therapeutic potential of lactoferrin-coated iron oxide nanospheres for targeted hyperthermia in gastric cancer.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
19 10 2023
19 10 2023
Historique:
received:
18
07
2023
accepted:
27
09
2023
medline:
23
10
2023
pubmed:
20
10
2023
entrez:
19
10
2023
Statut:
epublish
Résumé
Lactoferrin (LF) is a non-heme iron-binding glycoprotein involved in the transport of iron in blood plasma. In addition, it has many biological functions, including antibacterial, antiviral, antimicrobial, antiparasitic, and, importantly, antitumor properties. In this study, we have investigated the potential of employing lactoferrin-iron oxide nanoparticles (LF-IONPs) as a treatment modality for gastric cancer. The study confirms the formation of LF-IONPs with a spherical shape and an average size of 5 ± 2 nm, embedded within the protein matrix. FTIR and Raman analysis revealed that the Fe-O bond stabilized the protein particle interactions. Further, we conducted hyperthermia studies to ascertain whether the proposed composite can generate a sufficient rise in temperature at a low frequency. The results confirmed that we can achieve a temperature rise of about 7 °C at 242.4 kHz, which can be further harnessed for gastric cancer treatment. The particles were further tested for their anti-cancer activity on AGS cells, with and without hyperthermia. Results indicate that LF-IONPs (10 µg/ml) significantly enhance cytotoxicity, resulting in the demise of 67.75 ± 5.2% of cells post hyperthermia, while also exhibiting an inhibitory effect on cell migration compared to control cells, with the most inhibition observed after 36 h of treatment. These findings suggest the potential of LF-IONPs in targeted hyperthermia treatment of gastric cancer.
Identifiants
pubmed: 37857677
doi: 10.1038/s41598-023-43725-3
pii: 10.1038/s41598-023-43725-3
pmc: PMC10587155
doi:
Substances chimiques
ferric oxide
1K09F3G675
Lactoferrin
EC 3.4.21.-
Iron
E1UOL152H7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17875Informations de copyright
© 2023. Springer Nature Limited.
Références
RSC Adv. 2021 Jul 14;11(40):24656-24668
pubmed: 35481039
J Biol Inorg Chem. 2004 Sep;9(6):706-12
pubmed: 15232722
BMC Cancer. 2015 May 22;15:425
pubmed: 25998617
Jpn J Cancer Res. 2000 Jan;91(1):25-33
pubmed: 10744041
Dalton Trans. 2011 Jan 28;40(4):820-7
pubmed: 21152600
Biomaterials. 2008 Feb;29(4):487-96
pubmed: 17964647
J Chem Inf Model. 2016 Dec 27;56(12):2287-2291
pubmed: 27976886
Int J Biol Macromol. 2020 Dec 15;165(Pt A):970-984
pubmed: 33011258
Proc Natl Acad Sci U S A. 1989 Aug;86(16):6077-81
pubmed: 2762315
J Mol Biol. 1995 Apr 7;247(4):536-40
pubmed: 7723011
Invest New Drugs. 2021 Apr;39(2):400-415
pubmed: 33063290
Antimicrob Agents Chemother. 2012 Mar;56(3):1548-56
pubmed: 22214780
Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10915-9
pubmed: 1438297
J Am Chem Soc. 2016 Jun 29;138(25):7899-909
pubmed: 27263865
Apoptosis. 2019 Aug;24(7-8):562-577
pubmed: 30941553
Biomaterials. 2008 Oct;29(29):4012-21
pubmed: 18649936
Colloids Surf B Biointerfaces. 2020 Apr;188:110785
pubmed: 31951930
Arch Dis Child. 1992 May;67(5):657-61
pubmed: 1599309
Int J Hyperthermia. 2013 Dec;29(8):790-800
pubmed: 23968194
Strahlenther Onkol. 2012 Sep;188 Suppl 2:198-211
pubmed: 22926657
Biomolecules. 2020 Mar 15;10(3):
pubmed: 32183434
Nanomedicine. 2019 Jan;15(1):47-57
pubmed: 30213518
Sci Adv. 2022 Mar 11;8(10):eabh1419
pubmed: 35275724
Molecules. 2015 May 26;20(6):9703-31
pubmed: 26016555
Med Oncol. 2023 Mar 24;40(5):126
pubmed: 36961614
IET Nanobiotechnol. 2023 May;17(3):212-223
pubmed: 37083267
Proteins. 2006 May 15;63(3):636-43
pubmed: 16470805
BMC Complement Med Ther. 2020 Nov 23;20(1):355
pubmed: 33225921
Jpn J Cancer Res. 1999 Mar;90(3):262-7
pubmed: 10359039
Mol Immunol. 2003 Nov;40(7):395-405
pubmed: 14568385
Int J Nanomedicine. 2015 Dec 21;11:25-44
pubmed: 26730188
Biochem Biophys Res Commun. 2019 Jun 4;513(3):681-687
pubmed: 30982578
Med Oncol. 2022 Sep 7;39(12):196
pubmed: 36071293
Molecules. 2021 Jan 03;26(1):
pubmed: 33401580
J Am Chem Soc. 2018 Jan 10;140(1):211-218
pubmed: 29237264
MethodsX. 2019 Sep 14;6:2134-2140
pubmed: 31667112
Adv Mater. 2014 May;26(17):2694-8, 2016
pubmed: 24615901
Oncotarget. 2016 Sep 20;7(38):62144-62158
pubmed: 27556694
Biometals. 2004 Jun;17(3):231-4
pubmed: 15222470
Cell Mol Life Sci. 2005 Nov;62(22):2531-9
pubmed: 16261257
Nutr Rev. 2014 Dec;72(12):763-73
pubmed: 25406879
Mol Cancer Ther. 2005 Apr;4(4):612-24
pubmed: 15827335
IEEE Trans Magn. 2010 Jul 1;46(7):2523-2558
pubmed: 20930943
J Leukoc Biol. 1991 May;49(5):427-33
pubmed: 1849951
Front Pharmacol. 2018 Nov 13;9:1300
pubmed: 30483135
Immunol Cell Biol. 2008 Mar-Apr;86(3):277-88
pubmed: 18268518
J Phys Chem A. 2014 Dec 11;118(49):11641-51
pubmed: 25406092
Heliyon. 2019 May 20;5(5):e01648
pubmed: 31193473
Nanoscale. 2015 Oct 28;7(40):16890-8
pubmed: 26412614
J Dairy Sci. 2002 Sep;85(9):2065-74
pubmed: 12362437
J Pept Sci. 2008 Sep;14(9):1032-8
pubmed: 18425992
Kidney Int. 2000 May;57(5):2004-10
pubmed: 10792619
Artif Cells Nanomed Biotechnol. 2016;44(1):188-93
pubmed: 25020049
Molecules. 2016 Jun 11;21(6):
pubmed: 27294909
Drug Metab Pharmacokinet. 2004 Aug;19(4):245-63
pubmed: 15499193
Int J Antimicrob Agents. 2009 Apr;33(4):301.e1-8
pubmed: 18842395
Chemistry. 2018 Apr 6;24(20):5078-5081
pubmed: 29314358
Adv Mater. 2017 Jun;29(24):
pubmed: 28402594
Nanomaterials (Basel). 2020 Aug 07;10(8):
pubmed: 32784715
Biosensors (Basel). 2019 Feb 28;9(1):
pubmed: 30823455