Researches of calcium-activated chloride channel ANO1 intervening amyotrophic lateral sclerosis progression by activating EGFR and CaMKII signaling.
Amyotrophic lateral sclerosis
Anoctamin1
Calmodulin-dependent protein kinase II
Epidermal growth factor receptor
Journal
Brain research bulletin
ISSN: 1873-2747
Titre abrégé: Brain Res Bull
Pays: United States
ID NLM: 7605818
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
15
06
2023
revised:
02
10
2023
accepted:
17
10
2023
medline:
27
11
2023
pubmed:
20
10
2023
entrez:
20
10
2023
Statut:
ppublish
Résumé
ANO1 is closely correlated with the activation of EGFR and CaMKII, while EGFR and CaMKII show low activation in amyotrophic lateral sclerosis (ALS) models. Therefore, we designed experiments to verify that ANO1 may play a protective role on motor neurons in ALS by activating EGFR and CaMKII. The expression changes of ANO1, EGFR, CaMKII, pEGFR, and pCaMKII, cell survival status, and apoptosis were studied by western blot, real-time quantitative PCR, immunofluorescence, immunohistochemistry, CCK-8, and flow cytometry. The role of ANO1 in the ALS model by activating EGFR and CaMKII was studied by applying corresponding activators, inhibitors, gene silencing, and overexpression. In hSOD1 Our results suggest that ANO1 plays an important role in the survival of ALS motor neurons. ANO1 can increase cell activity and reduce apoptosis by activating EGFR and CaMKII signals.
Sections du résumé
BACKGROUND
BACKGROUND
ANO1 is closely correlated with the activation of EGFR and CaMKII, while EGFR and CaMKII show low activation in amyotrophic lateral sclerosis (ALS) models. Therefore, we designed experiments to verify that ANO1 may play a protective role on motor neurons in ALS by activating EGFR and CaMKII.
METHODS
METHODS
The expression changes of ANO1, EGFR, CaMKII, pEGFR, and pCaMKII, cell survival status, and apoptosis were studied by western blot, real-time quantitative PCR, immunofluorescence, immunohistochemistry, CCK-8, and flow cytometry. The role of ANO1 in the ALS model by activating EGFR and CaMKII was studied by applying corresponding activators, inhibitors, gene silencing, and overexpression.
RESULTS
RESULTS
In hSOD1
CONCLUSIONS
CONCLUSIONS
Our results suggest that ANO1 plays an important role in the survival of ALS motor neurons. ANO1 can increase cell activity and reduce apoptosis by activating EGFR and CaMKII signals.
Identifiants
pubmed: 37858681
pii: S0361-9230(23)00217-4
doi: 10.1016/j.brainresbull.2023.110792
pii:
doi:
Substances chimiques
ANO1 protein, mouse
0
Anoctamin-1
0
Calcium-Calmodulin-Dependent Protein Kinase Type 2
EC 2.7.11.17
Chloride Channels
0
ErbB Receptors
EC 2.7.10.1
Superoxide Dismutase
EC 1.15.1.1
Superoxide Dismutase-1
EC 1.15.1.1
Camk2d protein, mouse
EC 2.7.11.17
EGFR protein, mouse
EC 2.7.10.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110792Informations de copyright
Copyright © 2023. Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.