Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.

Superoxide Dismutase-1 / genetics Reproducibility of Results Superoxide Dismutase / genetics Blotting, Western Antibodies Immunoprecipitation Fluorescent Antibody Technique Zinc
SOD1 Superoxide dismutase 1 Superoxide dismutase [Cu-Zn] Uniprot ID P00441 Western blot antibody characterization antibody validation immunofluorescence immunoprecipitation

Journal

F1000Research
ISSN: 2046-1402
Titre abrégé: F1000Res
Pays: England
ID NLM: 101594320

Informations de publication

Date de publication:
2023
Historique:
accepted: 29 08 2023
pubmed: 20 10 2023
medline: 23 10 2023
entrez: 20 10 2023
Statut: epublish

Résumé

Superoxide dismutase [Cu-Zn] 1 (SOD1), is an antioxidant enzyme encoded by the gene

Identifiants

DOI: 10.12688/f1000research.132952.1 PMID: 37860271 PMC: PMC10582621
pubmed: 37860271
doi: 10.12688/f1000research.132952.1
pmc: PMC10582621
doi:

Substances chimiques

Superoxide Dismutase-1 EC 1.15.1.1
Superoxide Dismutase EC 1.15.1.1
Antibodies 0
Zinc J41CSQ7QDS

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

391

Subventions

Organisme : CIHR
ID : FDN154305
Pays : Canada

Informations de copyright

Copyright: © 2023 Ayoubi R et al.

Déclaration de conflit d'intérêts

Competing interests: For this project, the laboratory of Peter McPherson developed partnerships with high-quality antibody manufacturers and knockout cell line providers. The partners provide antibodies and knockout cell lines to the McPherson laboratory at no cost. These partners include: - Abcam- ABclonal -Aviva Systems Biology -Bio Techne -Cell Signalling Technology -Developmental Studies Hybridoma Bank -GeneTex – Horizon Discovery – Proteintech – Synaptic Systems –Thermo Fisher Scientific.

Références

Nucleic Acids Res. 2023 Jan 6;51(D1):D358-D367
pubmed: 36370112
F1000Res. 2023 Jul 25;12:884
pubmed: 37635943
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pubmed: 24692554
Front Neurosci. 2016 Nov 04;10:499
pubmed: 27867347
Nature. 1993 Jul 22;364(6435):362
pubmed: 8332197
Elife. 2019 Oct 15;8:
pubmed: 31612854
J Biol Chem. 2001 Oct 19;276(42):38388-93
pubmed: 11507097
Mech Ageing Dev. 2005 Mar;126(3):365-79
pubmed: 15664623
Proteomics. 2015 Sep;15(18):3163-8
pubmed: 25656970
J Biomol Tech. 2018 Jul;29(2):25-38
pubmed: 29805321
F1000Res. 2022 Aug 24;11:977
pubmed: 36415206
Nat Commun. 2014 Mar 19;5:3446
pubmed: 24647101
Cell. 2020 Jan 23;180(2):387-402.e16
pubmed: 31978347

Auteurs

Riham Ayoubi (R)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H34 2B4, Canada.

Walaa Alshafie (W)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H34 2B4, Canada.

Zhipeng You (Z)

The Neuro's Early Drug Discovery Unit (EDDU), Structural Genomics Consortium, McGill University, Montreal, Québec, H3A 2B4, Canada.

Kathleen Southern (K)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H34 2B4, Canada.
ORCID: 0000-0002-4125-3608

Peter S McPherson (PS)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H34 2B4, Canada.

Carl Laflamme (C)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H34 2B4, Canada.
ORCID: 0000-0001-5906-025X

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Superoxide dismutase › Superoxide dismutase-1 › Identification of high-performing antibodies...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Identification of high-performing antibodies...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Superoxide dismutase › Identification of high-performing antibodies...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Immunotransfert › Technique de Western › Identification of high-performing antibodies...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Identification of high-performing antibodies...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Immunoprécipitation › Identification of high-performing antibodies...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Technique d'immunofluorescence › Identification of high-performing antibodies...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Zinc › Identification of high-performing antibodies...