Validation of a new method for the detection of Ethyl glucuronide in larvae of
Entomotoxicology
Ethanol
Ethyl glucuronide
Forensic toxicology
Larvae
Ultra-high-performance liquid cromatography
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
26
04
2023
revised:
11
09
2023
accepted:
06
10
2023
medline:
20
10
2023
pubmed:
20
10
2023
entrez:
20
10
2023
Statut:
epublish
Résumé
Larvae and insects are an important and alternative biological matrix in the development anpost-mortem forensic toxicology. They are very useful when conventional matrices are not available, in particular when the loss of biological fluids, due to the decomposition of corpses or to a traumatic death, occurs. The purpose of this study is to develop and validate an analytical method in Ultra High-Performance Liquid Chromatography at High Resolution Mass Spectrometry (HPLC/HR-MS) for the research and quantification of Ethyl glucuronide (EtG) on larvae. The criteria taken into consideration for the validation are linearity, quantitation limits (LOD and LLOQ), accuracy, precision, carryover, interferences and ionization suppression/enhancement. The method was shown to be linear within the tested range, with a coefficient of determination higher than 0.99. LOD was 2 pg mg
Identifiants
pubmed: 37860567
doi: 10.1016/j.heliyon.2023.e20802
pii: S2405-8440(23)08010-6
pmc: PMC10582484
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e20802Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
J Anal Toxicol. 2021 Nov 9;45(9):961-968
pubmed: 33031530
J Anal Toxicol. 2021 Sep 17;45(8):878-884
pubmed: 34086901
Forensic Toxicol. 2022 Jan;40(1):144-155
pubmed: 36454495
Semin Diagn Pathol. 2009 Feb;26(1):18-27
pubmed: 19292025
Int J Legal Med. 2006 Mar;120(2):83-8
pubmed: 16059713
Int J Legal Med. 2001;114(4-5):197-203
pubmed: 11355395
Forensic Sci Int. 2001 Aug 15;120(1-2):42-7
pubmed: 11457608
J Anal Toxicol. 2018 Jun 1;42(5):346-352
pubmed: 29425299
J Forensic Leg Med. 2019 Oct;67:28-36
pubmed: 31398663
Drug Test Anal. 2022 Jan;14(1):92-100
pubmed: 34398533
Forensic Sci Int. 2007 Jan 5;165(1):10-29
pubmed: 16782292
Int J Legal Med. 2008 Sep;122(5):429-34
pubmed: 18594851
Ann Ist Super Sanita. 2015;51(1):19-27
pubmed: 25857380
Forensic Sci Int. 2011 May 20;208(1-3):1-9
pubmed: 21237593
Forensic Sci Int. 2007 Jan 5;165(1):41-5
pubmed: 16564658