Ultrastructural Reorganization of Endotheliocytes of Pulmonary Blood Capillaries in COVID-19.
COVID-19
endothelium
lungs
ultrastructure
Journal
Bulletin of experimental biology and medicine
ISSN: 1573-8221
Titre abrégé: Bull Exp Biol Med
Pays: United States
ID NLM: 0372557
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
21
02
2023
medline:
2
11
2023
pubmed:
20
10
2023
entrez:
20
10
2023
Statut:
ppublish
Résumé
The ultrastructural organization endotheliocytes of pulmonary blood capillaries in COVID-19 was studied on autopsy material using electron microscopy. Swelling of the cytoplasm and mitochondria with destruction of the cristae, dilation of the Golgi complex cisternae, a decrease in the volume density of the luminal and basal caveolae and free transport vesicles, an increase of the rough endoplasmic reticulum, as well as the presence of elements of coronavirus replication (reticulovesicular structures, zippered endoplasmic reticulum, electron-dense particles in the Golgi cisternae, and vacuoles with viral particles) were revealed. Further studies of the intracellular mechanisms used by the virus to replicate could help to develop antiviral drugs for the treatment of the new coronavirus infection.
Identifiants
pubmed: 37861904
doi: 10.1007/s10517-023-05923-x
pii: 10.1007/s10517-023-05923-x
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
667-672Informations de copyright
© 2023. Springer Science+Business Media, LLC, part of Springer Nature.
Références
Jiménez D, Torres Arias M. Immunouniverse of SARS-CoV-2. Immunol. Med. 2022;45(4):186-224. doi: https://doi.org/10.1080/25785826.2022.2066251
doi: 10.1080/25785826.2022.2066251
pubmed: 35502127
Peacock TP, Goldhill DH, Zhou J, Baillon L, Frise R, Swann OC, Kugathasan R, Penn R, Brown JC, Sanchez-David RY, Braga L, Williamson MK, Hassard JA, Staller E, Hanley B, Osborn M, Giacca M, Davidson AD, Matthews DA, Barclay WS. The furin cleavage site in the SARS-CoV-2 spike protein is required for transmission in ferrets. Nat. Microbiol. 2021;6(7):899-909. doi: https://doi.org/10.1038/s41564-021-00908-w
doi: 10.1038/s41564-021-00908-w
pubmed: 33907312
Scialo F, Daniele A, Amato F, Pastore L, Matera MG, Cazzola M, Castaldo G, Bianco A. ACE2: The major cell entry receptor for SARS-CoV-2. Lung. 2020;198(6):867-877. doi: https://doi.org/10.1007/s00408-020-00408-4
doi: 10.1007/s00408-020-00408-4
pubmed: 33170317
pmcid: 7653219
Castro P, Palomo M, Moreno-Castaño AB, Fernández S, Torramadé-Moix S, Pascual G, Martinez-Sanchez J, Richardson E, Téllez A, Nicolas JM, Carreras E, Richardson PG, Badimon JJ, Escolar G, Diaz-Ricart M. Is the endothelium the missing link in the pathophysiology and treatment of COVID-19 complications? Cardiovasc. Drugs Ther. 2022;36(3):547-560. doi: https://doi.org/10.1007/s10557-021-07207-w
doi: 10.1007/s10557-021-07207-w
pubmed: 34097193
Polak SB, Van Gool IC, Cohen D, von der Thüsen JH, van Paassen J. A systematic review of pathological findings in COVID-19: a pathophysiological timeline and possible mechanisms of disease progression. Mod. Pathol. 2020;33(11):2128-2138. doi: https://doi.org/10.1038/s41379-020-0603-3
doi: 10.1038/s41379-020-0603-3
pubmed: 32572155
pmcid: 7306927
Libby P, Lüscher T. COVID-19 is, in the end, an endothelial disease. Eur. Heart J. 2020;41(32):3038-3044. doi: https://doi.org/10.1093/eurheartj/ehaa623
doi: 10.1093/eurheartj/ehaa623
pubmed: 32882706
Menter T, Haslbauer JD, Nienhold R, Savic S, Hopfer H, Deigendesch N, Frank S, Turek D, Willi N, Pargger H, Bassetti S, Leuppi JD, Cathomas G, Tolnay M, Mertz KD, Tzankov A. Postmortem examination of COVID-19 patients reveals diffuse alveolar damage with severe capillary congestion and variegated findings in lungs and other organs suggesting vascular dysfunction. Histopathology. 2020;77(2):198-209. doi: https://doi.org/10.1111/his.14134
doi: 10.1111/his.14134
pubmed: 32364264
pmcid: 7496150
Investigation of Deceased with Suspected Coronavirus Infection (COVID-19): Guidelines. Frank GA, Kovalev AV, eds. Moscow, 2020. Russian.
Interim Guidelines. New Coronavirus Infection (COVID-19). Rules of Work of Pathology Departments. Pathological Anatomy of COVID-19. Ver. 2 (April 27, 2020). Moscow, 2020.
Dittmayer C, Meinhardt J, Radbruch H, Radke J, Heppner BI, Heppner FL, Stenzel W, Holland G, Laue M. Why misinterpretation of electron micrographs in SARS-CoV-2-infected tissue goes viral. Lancet. 2020;396:e64-e65. doi: https://doi.org/10.1016/S0140-6736(20)32079-1
doi: 10.1016/S0140-6736(20)32079-1
pubmed: 33031763
pmcid: 7535802
Hackstadt T, Chiramel AI, Hoyt FH, Williamson BN, Dooley CA, Beare PA, de Wit E, Best SM, Fischer ER. Disruption of the Golgi apparatus and contribution of the endoplasmic reticulum to the SARS-CoV-2 replication complex. Viruses. 2021;13(9):1798. doi: https://doi.org/10.3390/v13091798
doi: 10.3390/v13091798
pubmed: 34578379
pmcid: 8473243
Bagchi P. Endoplasmic reticulum in viral infection. Int. Rev. Cell Mol. Biol. 2020;350:265-284. doi: https://doi.org/10.1016/bs.ircmb.2019.10.005
doi: 10.1016/bs.ircmb.2019.10.005
pubmed: 32138901
Snijder EJ, Limpens RWAL, de Wilde AH, de Jong AWM, Zevenhoven-Dobbe JC, Maier HJ, Faas FFGA, Koster AJ, Bárcena M. A unifying structural and functional model of the coronavirus replication organelle: Tracking down RNA synthesis. PLoS Biol. 2020;18(6):e3000715. doi: https://doi.org/10.1371/journal.pbio.3000715
doi: 10.1371/journal.pbio.3000715
pubmed: 32511245
pmcid: 7302735
Caldas LA, Carneiro FA, Monteiro FL, Augusto I, Higa LM, Miranda K, Tanuri A, de Souza W. Intracellular host cell membrane remodelling induced by SARS-CoV-2 infection in vitro. Biol. Cell. 2021;113(6):281-293. doi: https://doi.org/10.1111/boc.202000146
doi: 10.1111/boc.202000146
pubmed: 33600624
pmcid: 8013410
Cortese M, Lee JY, Cerikan B, Neufeldt CJ, Oorschot VMJ, Köhrer S, Hennies J, Schieber NL, Ronchi P, Mizzon G, Romero-Brey I, Santarella-Mellwig R, Schorb M, Boermel M, Mocaer K, Beckwith MS, Templin RM, Gross V, Pape C, Tischer C, Frankish J, Horvat NK, Laketa V, Stanifer M, Boulant S, Ruggieri A, Chatel-Chaix L, Schwab Y, Bartenschlager R. Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologies. Cell Host Microbe. 2020;28(6):853-866.e5. doi: https://doi.org/10.1016/j.chom.2020.11.003
doi: 10.1016/j.chom.2020.11.003
pubmed: 33245857
pmcid: 7670925