Experimental transmission of ovine atypical scrapie to cattle.
PMCA
Transmissible spongiform encephalopathy
atypical scrapie
bovine
mouse bioassay
prion
veterinary neurology
Journal
Veterinary research
ISSN: 1297-9716
Titre abrégé: Vet Res
Pays: England
ID NLM: 9309551
Informations de publication
Date de publication:
20 Oct 2023
20 Oct 2023
Historique:
received:
27
06
2023
accepted:
11
09
2023
medline:
31
10
2023
pubmed:
21
10
2023
entrez:
20
10
2023
Statut:
epublish
Résumé
Classical bovine spongiform encephalopathy (BSE) in cattle was caused by the recycling and feeding of meat and bone meal contaminated with a transmissible spongiform encephalopathy (TSE) agent but its origin remains unknown. This study aimed to determine whether atypical scrapie could cause disease in cattle and to compare it with other known TSEs in cattle. Two groups of calves (five and two) were intracerebrally inoculated with atypical scrapie brain homogenate from two sheep with atypical scrapie. Controls were five calves intracerebrally inoculated with saline solution and one non-inoculated animal. Cattle were clinically monitored until clinical end-stage or at least 96 months post-inoculation (mpi). After euthanasia, tissues were collected for TSE diagnosis and potential transgenic mouse bioassay. One animal was culled with BSE-like clinical signs at 48 mpi. The other cattle either developed intercurrent diseases leading to cull or remained clinical unremarkable at study endpoint, including control cattle. None of the animals tested positive for TSEs by Western immunoblot and immunohistochemistry. Bioassay of brain samples from the clinical suspect in Ov-Tg338 and Bov-Tg110 mice was also negative. By contrast, protein misfolding cyclic amplification detected prions in the examined brains from atypical scrapie-challenged cattle, which had a classical BSE-like phenotype. This study demonstrates for the first time that a TSE agent with BSE-like properties can be amplified in cattle inoculated with atypical scrapie brain homogenate.
Identifiants
pubmed: 37864218
doi: 10.1186/s13567-023-01224-3
pii: 10.1186/s13567-023-01224-3
pmc: PMC10589953
doi:
Substances chimiques
Prions
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
98Subventions
Organisme : Department for Environment, Food and Rural Affairs, UK Government
ID : SE1869
Organisme : Department for Environment, Food and Rural Affairs, UK Government
ID : SE1960
Organisme : Department for Environment, Food and Rural Affairs, UK Government
ID : SE1961
Organisme : Department for Environment, Food and Rural Affairs, UK Government
ID : SE1962
Organisme : European Commission
ID : NADIR
Organisme : Ministerio de Ciencia e Innovación
ID : PID2019-105837RB-I00
Informations de copyright
© 2023. L’Institut National de Recherche en Agriculture, Alimentation et Environnement (INRAE).
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