Effects of hTERT transfection on the telomere and telomerase of Periplaneta americana cells in vitro.
Cell immortalization
Periplaneta americana
Telomerase
Telomere
hTERT
Journal
AMB Express
ISSN: 2191-0855
Titre abrégé: AMB Express
Pays: Germany
ID NLM: 101561785
Informations de publication
Date de publication:
21 Oct 2023
21 Oct 2023
Historique:
received:
29
09
2023
accepted:
03
10
2023
medline:
21
10
2023
pubmed:
21
10
2023
entrez:
21
10
2023
Statut:
epublish
Résumé
Telomere and telomerase are crucial factors in cell division and chromosome stability. Telomerase activity in most cells depends on the transcription control by the telomerase reverse transcriptase (TERT). The introduction of an exogenous human TERT (hTERT) in cultured cells could enhance telomerase activity and elongate the lifespan of various cells. Telomere elongation mechanisms vary between insects and are complex and unusual. Whether the use of exogenous hTERT can immortalize primary insect cells remains to be investigated. In this study, we used a recombinant virus expressing hTERT to infect primary cultured cells of Periplaneta americana and evaluated its effects on insect cell immortalization. We found that hTERT was successfully expressed and promoted the growth of P. americana cells, shortening their doubling time. This was due to the ability of hTERT to increase the activity of telomerase in P. americana cells, thus prolonging the telomeres. Our study lays the foundation for understanding the mechanisms of telomere elongation in P. americana, and suggests that the introduction of hTERT into insect cells could be an efficient way to establish certain insect cell lines.
Identifiants
pubmed: 37864620
doi: 10.1186/s13568-023-01624-w
pii: 10.1186/s13568-023-01624-w
pmc: PMC10590340
doi:
Types de publication
Journal Article
Langues
eng
Pagination
118Subventions
Organisme : National Natural Science Foundation of China
ID : 32170168
Organisme : Yunnan Fundamental Research Projects
ID : 2019FB043
Informations de copyright
© 2023. Springer-Verlag GmbH Germany, part of Springer Nature.
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