3D chromatin interactions involving Drosophila insulators are infrequent but preferential and arise before TADs and transcription.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
21 10 2023
21 10 2023
Historique:
received:
07
12
2022
accepted:
12
10
2023
medline:
1
11
2023
pubmed:
22
10
2023
entrez:
21
10
2023
Statut:
epublish
Résumé
In mammals, insulators contribute to the regulation of loop extrusion to organize chromatin into topologically associating domains. In Drosophila the role of insulators in 3D genome organization is, however, under current debate. Here, we addressed this question by combining bioinformatics analysis and multiplexed chromatin imaging. We describe a class of Drosophila insulators enriched at regions forming preferential chromatin interactions genome-wide. Notably, most of these 3D interactions do not involve TAD borders. Multiplexed imaging shows that these interactions occur infrequently, and only rarely involve multiple genomic regions coalescing together in space in single cells. Finally, we show that non-border preferential 3D interactions enriched in this class of insulators are present before TADs and transcription during Drosophila development. Our results are inconsistent with insulators forming stable hubs in single cells, and instead suggest that they fine-tune existing 3D chromatin interactions, providing an additional regulatory layer for transcriptional regulation.
Identifiants
pubmed: 37865700
doi: 10.1038/s41467-023-42485-y
pii: 10.1038/s41467-023-42485-y
pmc: PMC10590426
doi:
Substances chimiques
Chromatin
0
Drosophila Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6678Informations de copyright
© 2023. Springer Nature Limited.
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