[Effects of Hemoglobin Variants on Glycosylated Hemoglobin Testing].
Glycosylated hemoglobin
Hemoglobin variant
Retrospective analysis
Journal
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
ISSN: 1672-173X
Titre abrégé: Sichuan Da Xue Xue Bao Yi Xue Ban
Pays: China
ID NLM: 101162609
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
medline:
26
10
2023
pubmed:
23
10
2023
entrez:
22
10
2023
Statut:
ppublish
Résumé
To examine the common types of hemoglobin variants and to evaluate the influence of common variants on the results of two kinds of glycosylated hemoglobin (HbA1c) tests. We conducted a retrospective study, analyzing the data of a patient population undergoing two HbA1c tests, high performance liquid chromatography (HPLC) and capillary electrophoresis (CE), at West China Hospital, Sichuan University between March 2021 and February 2022. By screening the chromatograms, the hemoglobin variants were identified and their migration positions in the CE method were recorded. The effects of the variants with different migration positions on the findings of the two methods were compared. Variant samples with different migration positions were selected and Sanger sequencing was performed to determine mutations in HBA1, HBA2, and HBB genes in the variant samples. We examined the HbA1c of 207 786 patient samples, identifying variant peaks in the chromatograms of 372 patients. The detection rate of variants was 0.18%, with the variant identification rate of HPLC being 43.3% and that of CE, 100%. Through sequencing, 20 variants were detected. A total of 261 patient samples were tested for HbA1c with both HPLC and CE. HPLC reported all HbA1c results, while CE did not report HbA1c results for 28 samples, among which, 26 showed abnormal peaks that overlapped with HbA1c peaks, and 2 showed abnormal peaks that overlapped with HbA0 peaks. The commonly observed variant migration positions, as revealed by CE, were at the horizontal coordinates of 225±1, 200±3, 100±2, 124±1, 70±2, and 182±1. There was significant difference between HPLC method and CE method in the determination of HbA1c ( There are diverse types of hemoglobin variants and most of them can affect the HbA1c findings of HPLC. Analyzing the chromatogram facilitates the identification of the variants.
Identifiants
pubmed: 37866962
doi: 10.12182/20230960210
pmc: PMC10579089
doi:
Substances chimiques
Glycated Hemoglobin
0
Types de publication
English Abstract
Journal Article
Langues
chi
Sous-ensembles de citation
IM
Pagination
1019-1023Informations de copyright
Copyright© by Editorial Board of Journal of Sichuan University (Medical Sciences).
Références
Clin Chim Acta. 2018 Jan;476:67-74
pubmed: 29154790
Am J Clin Pathol. 2018 Jan 29;149(2):172-180
pubmed: 29365076
Med Sci (Basel). 2021 Jun 07;9(2):
pubmed: 34200315
Clin Chim Acta. 2022 Jun 1;531:145-151
pubmed: 35378091
Sci Rep. 2019 Jun 4;9(1):8264
pubmed: 31164695
Clin Chem Lab Med. 2020 Jul 20;59(1):227-232
pubmed: 32687481
Arch Pathol Lab Med. 2013 Dec;137(12):1788-91
pubmed: 24283860
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Aug;28(4):1316-1320
pubmed: 32798419
Clin Chim Acta. 2023 Jan 15;539:55-65
pubmed: 36476843
Clin Chem Lab Med. 2016 Jan;54(1):91-5
pubmed: 26053012
Indian J Clin Biochem. 2022 Apr;37(2):242-246
pubmed: 35463112
Biochem Med (Zagreb). 2016 Oct 15;26(3):353-364
pubmed: 27812304
Am J Clin Pathol. 2021 Aug 4;156(3):445-454
pubmed: 33791753
J Clin Lab Anal. 2020 Oct;34(10):e23446
pubmed: 32656855
Am J Clin Pathol. 2022 Jun 7;157(6):852-857
pubmed: 34871348
Scand J Clin Lab Invest. 2023 Feb;83(1):18-22
pubmed: 36534489
Clin Chem Lab Med. 2018 Jul 26;56(8):1353-1361
pubmed: 29626415