Physiological Replication of the Human Glomerulus Using a Triple Culture Microphysiological System.
cross-talk study
glomerulus-on-a-chip
mesangium
tri-culture model
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
11 2023
11 2023
Historique:
revised:
12
09
2023
received:
15
05
2023
medline:
27
11
2023
pubmed:
23
10
2023
entrez:
22
10
2023
Statut:
ppublish
Résumé
The function of the glomerulus depends on the complex cell-cell/matrix interactions and replication of this in vitro would aid biological understanding in both health and disease. Previous models do not fully reflect all cell types and interactions present as they overlook mesangial cells within their 3D matrix. Herein, the development of a microphysiological system that contains all resident renal cell types in an anatomically relevant manner is presented. A detailed transcriptomic analysis of the contributing biology of each cell type, as well as functionally appropriate albumin retention in the system, is demonstrated. The important role of mesangial cells is shown in promoting the health and maturity of the other cell types. Additionally, a comparison of the incremental advances that each individual cell type brings to the phenotype of the others demonstrates that glomerular cells in simple 2D culture exhibit a state more reflective of the dysfunction observed in human disease than previously recognized. This in vitro model will expand the capability to investigate glomerular biology in a more translatable manner by the inclusion of the important mesangial cell compartment.
Identifiants
pubmed: 37867234
doi: 10.1002/advs.202303131
pmc: PMC10667800
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2303131Subventions
Organisme : AstraZeneca
Informations de copyright
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
Références
Clin Exp Immunol. 2016 Feb;183(2):157-65
pubmed: 26459770
Micromachines (Basel). 2021 Aug 16;12(8):
pubmed: 34442589
J Biosci Bioeng. 2005 Jun;99(6):529-40
pubmed: 16233828
Front Immunol. 2019 Jun 26;10:1405
pubmed: 31297110
Cell Stem Cell. 2019 Sep 5;25(3):373-387.e9
pubmed: 31303547
Nat Biomed Eng. 2017;1:
pubmed: 29038743
Lupus. 2009 Apr;18(4):355-60
pubmed: 19276304
ACS Cent Sci. 2020 Jun 24;6(6):903-912
pubmed: 32607437
Nat Rev Nephrol. 2021 Jul;17(7):441-464
pubmed: 33767431
Kidney Res Clin Pract. 2013 Mar 1;32(1):3-10
pubmed: 23946930
Dev Cell. 2019 Jul 1;50(1):102-116.e6
pubmed: 31265809
PLoS One. 2019 Oct 3;14(10):e0222948
pubmed: 31581251
Kidney Int. 2018 May;93(5):1086-1097
pubmed: 29433915
Sci Rep. 2016 Aug 25;6:31771
pubmed: 27558173
Am J Pathol. 2008 Oct;173(4):938-48
pubmed: 18772335
Nephrol Dial Transplant. 2007 Dec;22(12):3442-50
pubmed: 17686813
Adv Sci (Weinh). 2023 Nov;10(33):e2303131
pubmed: 37867234
Front Med (Lausanne). 2015 Mar 23;2:16
pubmed: 25853135
J Clin Invest. 2004 Nov;114(10):1412-4
pubmed: 15545991
J Pathol. 2017 Nov;243(3):390-400
pubmed: 28815607
Transl Res. 2006 Sep;148(3):142-8
pubmed: 16938652
Front Med (Lausanne). 2022 Jan 26;8:740527
pubmed: 35155460
Nat Rev Nephrol. 2022 Apr;18(4):241-257
pubmed: 35064233
Nat Rev Nephrol. 2019 Oct;15(10):613-624
pubmed: 31383997
Diabetes. 1999 Nov;48(11):2229-39
pubmed: 10535459
Curr Opin Nephrol Hypertens. 2015 May;24(3):231-8
pubmed: 25887901
Kidney Int. 2001 Jun;59(6):2104-13
pubmed: 11380812
J Immunol. 2011 Dec 1;187(11):5500-4
pubmed: 22031763
J Am Soc Nephrol. 2014 Sep;25(9):1966-78
pubmed: 24652804
Am J Pathol. 2008 Feb;172(2):299-308
pubmed: 18202193
Semin Nephrol. 2003 Nov;23(6):532-43
pubmed: 14631561
Cell Physiol Biochem. 2012;29(5-6):743-52
pubmed: 22613975
Pediatr Nephrol. 2017 Dec;32(12):2215-2224
pubmed: 28293726
J Am Soc Nephrol. 2008 Jul;19(7):1276-81
pubmed: 18287560
Biofabrication. 2023 Apr 12;15(3):
pubmed: 36898152
Nat Protoc. 2018 Jul;13(7):1662-1685
pubmed: 29995874
J Am Soc Nephrol. 2009 Jun;20(6):1179-87
pubmed: 19470685
Exp Cell Res. 2017 Apr 1;353(1):16-25
pubmed: 28237245
J Membr Biol. 1977 Jun 3;34(1):93-101
pubmed: 894702
J Immunol. 2008 Apr 15;180(8):5653-61
pubmed: 18390750
Kidney Int. 2008 Mar;73(5):556-66
pubmed: 18033240
J Immunol. 2014 Jul 1;193(1):234-43
pubmed: 24872191
Nat Med. 2003 Jul;9(7):964-8
pubmed: 12808448
Hum Pathol. 2016 Mar;49:135-44
pubmed: 26826420
J Pathol. 2020 Sep;252(1):88-100
pubmed: 32652570
Lab Chip. 2023 Jul 12;23(14):3226-3237
pubmed: 37341452
Kidney Int. 1994 Feb;45(2):320-7
pubmed: 8164415
Kidney Int. 2019 Aug;96(2):397-408
pubmed: 31142442
Kidney Int. 2018 Dec;94(6):1099-1110
pubmed: 30072040
Lab Chip. 2019 Jan 29;19(3):410-421
pubmed: 30663729
Adv Chronic Kidney Dis. 2014 Sep;21(5):408-16
pubmed: 25168829
Biomedicines. 2021 Feb 03;9(2):
pubmed: 33546224
Scand J Urol Nephrol. 2008;42(3):278-85
pubmed: 17943640
Sci Rep. 2018 Apr 12;8(1):5898
pubmed: 29651108
Kidney Int. 1997 Sep;52(3):761-70
pubmed: 9291198
Clin Exp Immunol. 1996 Jul;105(1):104-11
pubmed: 8697616
Nat Commun. 2019 Aug 13;10(1):3656
pubmed: 31409793
J Am Soc Nephrol. 2022 Jun;33(6):1120-1136
pubmed: 35292439
Clin Kidney J. 2015 Jun;8(3):343-50
pubmed: 26034600
Kidney Int Rep. 2019 Nov 13;5(2):211-224
pubmed: 32043035
Nat Biotechnol. 2010 May;28(5):436-40
pubmed: 20458311
Nat Rev Nephrol. 2018 Jun;14(6):378-393
pubmed: 29626199
J Hum Hypertens. 2014 Feb;28(2):74-9
pubmed: 23803592
Lab Chip. 2017 May 16;17(10):1749-1760
pubmed: 28418422