Perfusion Window Chambers Enable Interventional Analyses of Tumor Microenvironments.
TME
cancer
drug development
imaging
microscopy
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
revised:
29
09
2023
received:
18
07
2023
medline:
11
12
2023
pubmed:
23
10
2023
entrez:
23
10
2023
Statut:
ppublish
Résumé
Intravital microscopy (IVM) allows spatial and temporal imaging of different cell types in intact live tissue microenvironments. IVM has played a critical role in understanding cancer biology, invasion, metastases, and drug development. One considerable impediment to the field is the inability to interrogate the tumor microenvironment and its communication cascades during disease progression and therapeutic interventions. Here, a new implantable perfusion window chamber (PWC) is described that allows high-fidelity in vivo microscopy, local administration of stains and drugs, and longitudinal sampling of tumor interstitial fluid. This study shows that the new PWC design allows cyclic multiplexed imaging in vivo, imaging of drug action, and sampling of tumor-shed materials. The PWC will be broadly useful as a novel perturbable in vivo system for deciphering biology in complex microenvironments.
Identifiants
pubmed: 37870204
doi: 10.1002/advs.202304886
pmc: PMC10700240
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2304886Subventions
Organisme : NCI NIH HHS
ID : P01CA240239
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA069246
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA079443
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA281735
Pays : United States
Organisme : NCI NIH HHS
ID : T32CA079443
Pays : United States
Organisme : NCI NIH HHS
ID : R33CA277820
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA240239
Pays : United States
Organisme : NCI NIH HHS
ID : R33 CA277820
Pays : United States
Organisme : Swiss National Science Foundation
ID : P2EZP2_191831
Pays : Switzerland
Organisme : NCI NIH HHS
ID : R01CA281735
Pays : United States
Informations de copyright
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
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