Staphylococcus aureus sacculus mediates activities of M23 hydrolases.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
23 10 2023
23 10 2023
Historique:
received:
13
04
2023
accepted:
12
10
2023
medline:
27
10
2023
pubmed:
24
10
2023
entrez:
23
10
2023
Statut:
epublish
Résumé
Peptidoglycan, a gigadalton polymer, functions as the scaffold for bacterial cell walls and provides cell integrity. Peptidoglycan is remodelled by a large and diverse group of peptidoglycan hydrolases, which control bacterial cell growth and division. Over the years, many studies have focused on these enzymes, but knowledge on their action within peptidoglycan mesh from a molecular basis is scarce. Here, we provide structural insights into the interaction between short peptidoglycan fragments and the entire sacculus with two evolutionarily related peptidases of the M23 family, lysostaphin and LytM. Through nuclear magnetic resonance, mass spectrometry, information-driven modelling, site-directed mutagenesis and biochemical approaches, we propose a model in which peptidoglycan cross-linking affects the activity, selectivity and specificity of these two structurally related enzymes differently.
Identifiants
pubmed: 37872144
doi: 10.1038/s41467-023-42506-w
pii: 10.1038/s41467-023-42506-w
pmc: PMC10593780
doi:
Substances chimiques
Peptidoglycan
0
Hydrolases
EC 3.-
Lysostaphin
EC 3.4.24.75
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6706Informations de copyright
© 2023. Springer Nature Limited.
Références
Antimicrob Agents Chemother. 1993 Feb;37(2):342-6
pubmed: 8452368
EMBO J. 1996 Sep 16;15(18):4789-97
pubmed: 8890152
Front Mol Biosci. 2018 Jul 03;5:60
pubmed: 30018958
Nat Microbiol. 2020 Feb;5(2):291-303
pubmed: 31932712
Bacteriol Rev. 1972 Dec;36(4):407-77
pubmed: 4568761
Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):905-21
pubmed: 9757107
J Biol Chem. 1997 Nov 14;272(46):29053-9
pubmed: 9360979
Nat Commun. 2020 Jan 15;11(1):285
pubmed: 31941905
Curr Opin Microbiol. 2011 Dec;14(6):698-703
pubmed: 22055466
J Mol Biol. 2016 Feb 22;428(4):720-725
pubmed: 26410586
Anal Biochem. 1988 May 15;171(1):141-4
pubmed: 3407910
Front Microbiol. 2019 Feb 28;10:331
pubmed: 30873139
J Biol Chem. 1967 Jul 10;242(13):3207-17
pubmed: 6027794
Protein Eng. 1995 Feb;8(2):127-34
pubmed: 7630882
Sci Rep. 2019 Mar 21;9(1):5010
pubmed: 30899062
J Am Chem Soc. 2014 Dec 24;136(51):17852-60
pubmed: 25429710
J Biomol NMR. 2016 Oct;66(2):111-124
pubmed: 27663422
J Bacteriol. 2006 Apr;188(7):2463-72
pubmed: 16547033
J Mol Biol. 2009 Apr 3;387(3):607-18
pubmed: 19361422
J Am Chem Soc. 2010 Aug 11;132(31):10911-9
pubmed: 20681725
Nat Protoc. 2007;2(11):2728-33
pubmed: 18007608
J Mol Biol. 2004 Jan 16;335(3):775-85
pubmed: 14687573
PLoS Comput Biol. 2021 Apr 1;17(4):e1008889
pubmed: 33793553
Lett Appl Microbiol. 2010 Sep;51(3):358-61
pubmed: 20618890
J Am Chem Soc. 2008 Apr 30;130(17):5618-9
pubmed: 18393418
Biochim Biophys Acta. 2015 Jan;1848(1 Pt B):350-62
pubmed: 24915020
Antibiotics (Basel). 2020 Dec 17;9(12):
pubmed: 33348544
J Bacteriol. 2000 May;182(9):2635-8
pubmed: 10762270
Eur J Biochem. 1973 Oct 5;38(2):293-300
pubmed: 4773876
Sci Rep. 2015 Oct 06;5:14833
pubmed: 26437833
Cell Host Microbe. 2015 Nov 11;18(5):604-12
pubmed: 26567511
Sci Rep. 2014 Dec 16;4:7494
pubmed: 25510564
Mol Microbiol. 2005 Feb;55(3):799-807
pubmed: 15661005
Eur J Biochem. 1990 Jul 31;191(2):373-7
pubmed: 2384086
Nat Chem Biol. 2020 Jan;16(1):24-30
pubmed: 31686030
J Bacteriol. 1997 Jul;179(13):4311-8
pubmed: 9209049
Microbiology (Reading). 1999 Apr;145 ( Pt 4):801-808
pubmed: 10220159
Front Microbiol. 2019 Jan 18;9:3223
pubmed: 30713527
Angew Chem Int Ed Engl. 2010 Nov 2;49(45):8346-57
pubmed: 20941715
Infect Immun. 1994 Apr;62(4):1406-9
pubmed: 7907580
Magn Reson (Gott). 2020 Dec 23;1(2):331-345
pubmed: 37904819
Nature. 2020 Jun;582(7811):294-297
pubmed: 32523118
Org Biomol Chem. 2006 Oct 7;4(19):3626-38
pubmed: 16990938
J Mol Biol. 2010 Mar 5;396(4):908-23
pubmed: 20026068
J Biomol NMR. 2011 Jan;49(1):9-15
pubmed: 21190063
FEBS J. 2014 Sep;281(18):4112-22
pubmed: 25039253
Proteomics. 2006 Aug;6(15):4246-58
pubmed: 16826566
Trends Microbiol. 2010 Feb;18(2):59-66
pubmed: 20060721
Appl Environ Microbiol. 1995 Apr;61(4):1475-9
pubmed: 7747966
J Bacteriol. 2005 Mar;187(5):1815-24
pubmed: 15716453
Nucleic Acids Res. 2022 Jan 7;50(D1):D543-D552
pubmed: 34723319
Proc Natl Acad Sci U S A. 1964 Mar;51:414-21
pubmed: 14171453
FEMS Microbiol Rev. 2008 Mar;32(2):259-86
pubmed: 18266855
FEMS Microbiol Rev. 2007 Nov;31(6):676-91
pubmed: 17888003
Sci Adv. 2020 Sep 2;6(36):
pubmed: 32917596
mBio. 2015 Jul 28;6(4):e00660
pubmed: 26220963
Microb Drug Resist. 2016 Sep;22(6):461-9
pubmed: 27351490
J Bacteriol. 2006 Feb;188(3):1011-21
pubmed: 16428405
Int J Mol Sci. 2021 Jul 01;22(13):
pubmed: 34281200