Delivery of A Jagged1-PEG-MAL hydrogel with Pediatric Human Bone Cells Regenerates Critically-Sized Craniofacial Bone Defects.
Bone Regeneration
Craniofacial Bone Defects
Human Bone-derived Osteoblast-like Cells
JAGGED1
Non-canonical JAG1-NOTCH pathways
PEG-4MAL Hydrogel-based delivery
RNA Sequencing
Journal
bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187
Informations de publication
Date de publication:
10 Oct 2023
10 Oct 2023
Historique:
medline:
24
10
2023
pubmed:
24
10
2023
entrez:
24
10
2023
Statut:
epublish
Résumé
Treatments for congenital and acquired craniofacial (CF) bone abnormalities are limited and expensive. Current reconstructive methods include surgical correction of injuries, short-term bone stabilization, and long-term use of bone grafting solutions, including implantation of (i) allografts which are prone to implant failure or infection, (ii) autografts which are limited in supply. Current bone regenerative approaches have consistently relied on BMP-2 application with or without addition of stem cells. BMP2 treatment can lead to severe bony overgrowth or uncontrolled inflammation, which can accelerate further bone loss. Bone marrow-derived mesenchymal stem cell-based treatments, which do not have the side effects of BMP2, are not currently FDA approved, and are time and resource intensive. There is a critical need for novel bone regenerative therapies to treat CF bone loss that have minimal side effects, are easily available, and are affordable. In this study we investigated novel bone regenerative therapies downstream of JAGGED1 (JAG1). We previously demonstrated that JAG1 induces murine cranial neural crest (CNC) cells towards osteoblast commitment via a NOTCH non-canonical pathway involving JAK2-STAT5 (1) and that JAG1 delivery with CNC cells elicits bone regeneration
Identifiants
pubmed: 37873448
doi: 10.1101/2023.10.06.561291
pmc: PMC10592619
pii:
doi:
Types de publication
Preprint
Langues
eng
Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR062368
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR062920
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE031271
Pays : United States
Organisme : NIDCR NIH HHS
ID : R56 DE026762
Pays : United States
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