Enhancing classical swine fever virus identification: the advantages of Field-LAMP testing.
LAMP
POC tool
classical swine fever
electro-free
field test
rapid detection
Journal
Australian veterinary journal
ISSN: 1751-0813
Titre abrégé: Aust Vet J
Pays: England
ID NLM: 0370616
Informations de publication
Date de publication:
24 Oct 2023
24 Oct 2023
Historique:
revised:
22
09
2023
received:
04
08
2023
accepted:
07
10
2023
medline:
25
10
2023
pubmed:
25
10
2023
entrez:
24
10
2023
Statut:
aheadofprint
Résumé
Classical swine fever virus (CSFV) identification has witnessed significant advancements with the development of rapid reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assays. However, conventional RT-LAMP assays for CSFV diagnosis are hindered by a laborious RNA extraction step. Moreover, the need for thermal incubators and expensive micropipettes has limited their application in field settings. Addressing these challenges, our study presents a groundbreaking solution-an electro-free and point-of-care (POC) tool known as the field-LAMP assay-for the rapid clinical detection of CSFV. By eliminating the RNA extraction requirement, advancing the colorimetric read-out and lyophilized reaction reagents, our field-LAMP assay streamlines the diagnostic process, saving valuable time and effort. This novel approach also overcomes the dependency on electric-dependent thermal incubators and expensive micropipettes, making it practical and accessible for use in the field. The successful development of the field-LAMP assay marks a significant milestone in CSFV detection. This electro-free and POC tool offers several advantages, including its ability to deliver rapid results without compromising accuracy, facilitating prompt response and containment measures.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Nguyen Tat Thanh University
ID : 2022.01.132
Informations de copyright
© 2023 Australian Veterinary Association.
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