Development and qualification of clinical grade decellularized and cryopreserved human esophagi.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
25 10 2023
25 10 2023
Historique:
received:
30
05
2023
accepted:
21
10
2023
medline:
27
10
2023
pubmed:
26
10
2023
entrez:
25
10
2023
Statut:
epublish
Résumé
Tissue engineering is a promising alternative to current full thickness circumferential esophageal replacement methods. The aim of our study was to develop a clinical grade Decellularized Human Esophagus (DHE) for future clinical applications. After decontamination, human esophagi from deceased donors were placed in a bioreactor and decellularized with sodium dodecyl sulfate (SDS) and ethylendiaminetetraacetic acid (EDTA) for 3 days. The esophagi were then rinsed in sterile water and SDS was eliminated by filtration on an activated charcoal cartridge for 3 days. DNA was removed by a 3-hour incubation with DNase. A cryopreservation protocol was evaluated at the end of the process to create a DHE cryobank. The decellularization was efficient as no cells and nuclei were observed in the DHE. Sterility of the esophagi was obtained at the end of the process. The general structure of the DHE was preserved according to immunohistochemical and scanning electron microscopy images. SDS was efficiently removed, confirmed by a colorimetric dosage, lack of cytotoxicity on Balb/3T3 cells and mesenchymal stromal cell long term culture. Furthermore, DHE did not induce lymphocyte proliferation in-vitro. The cryopreservation protocol was safe and did not affect the tissue, preserving the biomechanical properties of the DHE. Our decellularization protocol allowed to develop the first clinical grade human decellularized and cryopreserved esophagus.
Identifiants
pubmed: 37880340
doi: 10.1038/s41598-023-45610-5
pii: 10.1038/s41598-023-45610-5
pmc: PMC10600094
doi:
Substances chimiques
Sodium Dodecyl Sulfate
368GB5141J
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18283Informations de copyright
© 2023. Springer Nature Limited.
Références
Expert Rev Med Devices. 2014 Mar;11(2):225-41
pubmed: 24387697
Sci Rep. 2015 Dec 03;5:17635
pubmed: 26631592
Anal Biochem. 1978 Nov;91(1):230-5
pubmed: 9762103
Cell Transplant. 2017 Dec;26(12):1831-1839
pubmed: 29390879
Surgery. 1983 Jan;93(1 Pt 1):70-7
pubmed: 6217567
Lancet. 2018 Mar 3;391(10123):883-910
pubmed: 28987452
Immunol Today. 1993 Oct;14(10):480-2
pubmed: 7506033
Biomaterials. 2018 Aug;175:1-18
pubmed: 29793088
Immunopharmacology. 1992 Sep-Oct;24(2):149-60
pubmed: 1473965
Glycobiology. 2003 Sep;13(9):647-53
pubmed: 12773478
Biochem J. 1968 Oct;109(5):825-30
pubmed: 4177067
J Intensive Care Med. 2010 May-Jun;25(3):139-48
pubmed: 20444738
Biomaterials. 2011 Apr;32(12):3233-43
pubmed: 21296410
Curr Top Dev Biol. 2018;130:1-37
pubmed: 29853174
J Crit Care. 2011 Apr;26(2):225.e1-10
pubmed: 20951541
Nat Commun. 2018 Oct 16;9(1):4286
pubmed: 30327457
Arthritis Res Ther. 2020 Dec 7;22(1):283
pubmed: 33287871
Bioengineering (Basel). 2018 Dec 24;6(1):
pubmed: 30586900
Semin Cell Dev Biol. 2002 Oct;13(5):377-83
pubmed: 12324220
Tissue Eng. 2006 Feb;12(2):319-30
pubmed: 16548690
Ann N Y Acad Sci. 2018 Dec;1434(1):156-163
pubmed: 30088660
Am J Transplant. 2018 Aug;18(8):1857-1864
pubmed: 29513380
Tissue Eng Part A. 2011 Oct;17(19-20):2435-43
pubmed: 21563860
PLoS One. 2017 Jun 9;12(6):e0179341
pubmed: 28599006
J Biol Chem. 2012 Mar 30;287(14):11363-73
pubmed: 22298772
Surgery. 2022 Feb;171(2):384-392
pubmed: 34392978
J Res Natl Inst Stand Technol. 2013 Apr 11;118:151-67
pubmed: 26401428
J Vasc Surg. 2021 Feb;73(2):626-634
pubmed: 33485491
Biomed Rep. 2015 Sep;3(5):617-620
pubmed: 26405534
J Immunol Methods. 2002 Jun 1;264(1-2):37-43
pubmed: 12191507
Clin Infect Dis. 2016 Feb 15;62(4):e1-50
pubmed: 26679628
Artif Organs. 2010 Mar;34(3):206-10
pubmed: 20447045
Biores Open Access. 2020 Feb 21;9(1):22-36
pubmed: 32117597
Anal Biochem. 1988 Jul;172(1):259-63
pubmed: 3189769
Therap Adv Gastroenterol. 2020 May 26;13:1756284820923220
pubmed: 32523626
Cells. 2022 Sep 20;11(19):
pubmed: 36230907
Xenotransplantation. 2015 Mar-Apr;22(2):95-101
pubmed: 25641488
Tissue Eng Part A. 2019 Nov;25(21-22):1478-1492
pubmed: 30799779
Surgery. 2010 Jul;148(1):39-47
pubmed: 20117812
J Thorac Cardiovasc Surg. 2004 Feb;127(2):399-405
pubmed: 14762347
J Tissue Eng Regen Med. 2019 Dec;13(12):2191-2203
pubmed: 31670903
J Chir (Paris). 2009 Jun;146(3):240-9
pubmed: 19640531