A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition.
capillary electrophoresis
plant sap
sampling
stress
xylem
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
25 Oct 2023
25 Oct 2023
Historique:
revised:
13
10
2023
received:
14
09
2023
accepted:
16
10
2023
pubmed:
26
10
2023
medline:
26
10
2023
entrez:
26
10
2023
Statut:
aheadofprint
Résumé
In this work, an online sampling of plant xylem sap combined with an efficient (CE)-based method was developed and applied to study the kinetics of changes in the sap composition and to assess plant fitness under stress conditions comprehensively. A laboratory-built CE device was developed to provide online sampling and CE analysis of various ionogenic species in the sap during plant stress response. The rapid online sampling and short CE analysis time allow for real-time monitoring of changes in sap constituents in the living plant during the stress response. The developed device was successfully used to analyze chloride, nitrate, and sulfate ions in the plant xylem during the salt stress or stress caused by nitrate deficiency within short time scales.
Identifiants
pubmed: 37880866
doi: 10.1002/elps.202300201
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ústav analytické chemie, Akademie Věd České Republiky
ID : RVO:68081715
Organisme : European Regional Development Fund
ID : CZ.02.1.01/0.0/0.0/16_026/0008446
Informations de copyright
© 2023 The Authors. Electrophoresis published by Wiley-VCH GmbH.
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