Application of elastography to diagnose adenomyosis and evaluate the degree of dysmenorrhea: a prospective observational study.
Adenomyosis
Diagnosis
Dysmenorrhea
Elastography
Fibrosis
Journal
Reproductive biology and endocrinology : RB&E
ISSN: 1477-7827
Titre abrégé: Reprod Biol Endocrinol
Pays: England
ID NLM: 101153627
Informations de publication
Date de publication:
26 Oct 2023
26 Oct 2023
Historique:
received:
24
08
2023
accepted:
24
09
2023
medline:
30
10
2023
pubmed:
27
10
2023
entrez:
26
10
2023
Statut:
epublish
Résumé
To determine whether there is a correlation between stiffness measured by strain elastography and the severity of dysmenorrhea and to determine the value of elastography in evaluating severe dysmenorrhea in patients with adenomyosis. The correlation between tissue stiffness and dysmenorrhea was analyzed by performing elastography on premenopausal women diagnosed with adenomyosis. Expression levels of transforming growth factor-β (TGF-β), α-smooth muscle actin (α-SMA), and protein gene product 9.5 (PGP9.5) were detected by immunohistochemistry; the correlation of TGF-β and α-SMA levels with the tissue stiffness and the degree of fibrosis was further analyzed. Also, the relationship of the PGP9.5 expression level with the tissue stiffness and degree of dysmenorrhea was determined. The degree of dysmenorrhea was significantly positively correlated with lesion stiffness in patients with adenomyosis but not with the uterine or lesion volume. The cutoff for the strain ratio was > 1.36 between the adenomyosis and control groups, with an area under the curve (AUC) of 0.987. For severe dysmenorrhea, the cutoff for the strain ratio was > 1.65 in patients with adenomyosis, with an AUC of 0.849. TGF-β, α-SMA, and PGP9.5 expression levels were higher in adenomyotic lesions than in the endometrium of the adenomyosis and control groups. Both TGF-β and α-SMA levels were positively correlated with the tissue stiffness and degree of fibrosis. Additionally, the expression level of PGP9.5 showed a positive correlation with the tissue stiffness and degree of dysmenorrhea. Elastography can be used to evaluate the degree of dysmenorrhea; the greater the tissue stiffness, the greater the degree of dysmenorrhea. In addition, elastography performed well in the diagnosis of adenomyosis and the evaluation of severe dysmenorrhea in patients with adenomyosis.
Sections du résumé
BACKGROUND
BACKGROUND
To determine whether there is a correlation between stiffness measured by strain elastography and the severity of dysmenorrhea and to determine the value of elastography in evaluating severe dysmenorrhea in patients with adenomyosis.
METHODS
METHODS
The correlation between tissue stiffness and dysmenorrhea was analyzed by performing elastography on premenopausal women diagnosed with adenomyosis. Expression levels of transforming growth factor-β (TGF-β), α-smooth muscle actin (α-SMA), and protein gene product 9.5 (PGP9.5) were detected by immunohistochemistry; the correlation of TGF-β and α-SMA levels with the tissue stiffness and the degree of fibrosis was further analyzed. Also, the relationship of the PGP9.5 expression level with the tissue stiffness and degree of dysmenorrhea was determined.
RESULTS
RESULTS
The degree of dysmenorrhea was significantly positively correlated with lesion stiffness in patients with adenomyosis but not with the uterine or lesion volume. The cutoff for the strain ratio was > 1.36 between the adenomyosis and control groups, with an area under the curve (AUC) of 0.987. For severe dysmenorrhea, the cutoff for the strain ratio was > 1.65 in patients with adenomyosis, with an AUC of 0.849. TGF-β, α-SMA, and PGP9.5 expression levels were higher in adenomyotic lesions than in the endometrium of the adenomyosis and control groups. Both TGF-β and α-SMA levels were positively correlated with the tissue stiffness and degree of fibrosis. Additionally, the expression level of PGP9.5 showed a positive correlation with the tissue stiffness and degree of dysmenorrhea.
CONCLUSIONS
CONCLUSIONS
Elastography can be used to evaluate the degree of dysmenorrhea; the greater the tissue stiffness, the greater the degree of dysmenorrhea. In addition, elastography performed well in the diagnosis of adenomyosis and the evaluation of severe dysmenorrhea in patients with adenomyosis.
Identifiants
pubmed: 37884924
doi: 10.1186/s12958-023-01145-y
pii: 10.1186/s12958-023-01145-y
pmc: PMC10601167
doi:
Substances chimiques
Transforming Growth Factor beta
0
Types de publication
Observational Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
98Subventions
Organisme : National Key Research and Development Program of China
ID : No. 2022YFC2704000
Organisme : National Key Research and Development Program of China
ID : No. 2022YFC2704000
Organisme : National Key Research and Development Program of China
ID : No. 2022YFC2704000
Organisme : National Key Research and Development Program of China
ID : No. 2022YFC2704000
Organisme : National Key Research and Development Program of China
ID : No. 2022YFC2704000
Organisme : National Key Research and Development Program of China
ID : No. 2022YFC2704000
Organisme : National Key Research and Development Program of China
ID : No. 2022YFC2704000
Organisme : National Key Research and Development Program of China
ID : No. 2022YFC2704000
Organisme : National Key Research and Development Program of China
ID : No. 2022YFC2704000
Organisme : National Natural Science Foundation of China
ID : 82071621
Organisme : National Natural Science Foundation of China
ID : 82071621
Organisme : National Natural Science Foundation of China
ID : 82071621
Organisme : National Natural Science Foundation of China
ID : 82071621
Organisme : National Natural Science Foundation of China
ID : 82071621
Organisme : National Natural Science Foundation of China
ID : 82071621
Organisme : National Natural Science Foundation of China
ID : 82071621
Organisme : National Natural Science Foundation of China
ID : 82071621
Organisme : National Natural Science Foundation of China
ID : 82071621
Organisme : Major Basic Research of Natural Science Foundation of Shandong Province
ID : ZR2021ZD34
Organisme : Major Basic Research of Natural Science Foundation of Shandong Province
ID : ZR2021ZD34
Organisme : Major Basic Research of Natural Science Foundation of Shandong Province
ID : ZR2021ZD34
Organisme : Major Basic Research of Natural Science Foundation of Shandong Province
ID : ZR2021ZD34
Organisme : Major Basic Research of Natural Science Foundation of Shandong Province
ID : ZR2021ZD34
Organisme : Major Basic Research of Natural Science Foundation of Shandong Province
ID : ZR2021ZD34
Organisme : Major Basic Research of Natural Science Foundation of Shandong Province
ID : ZR2021ZD34
Organisme : Major Basic Research of Natural Science Foundation of Shandong Province
ID : ZR2021ZD34
Organisme : Major Basic Research of Natural Science Foundation of Shandong Province
ID : ZR2021ZD34
Informations de copyright
© 2023. The Author(s).
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