Rapid and Label-Free Analysis of Antigen-Antibody Dynamic Binding of Tumor Markers Using Piezoelectric Quartz Crystal Biosensor.
binding kinetics
cancer
clinical serum samples
molecular diagnostics
piezoelectric (PZ) quartz crystal biosensor
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
07 Oct 2023
07 Oct 2023
Historique:
received:
23
08
2023
revised:
26
09
2023
accepted:
05
10
2023
medline:
30
10
2023
pubmed:
27
10
2023
entrez:
27
10
2023
Statut:
epublish
Résumé
Quantitative biomacromolecular diagnosis is rapidly developing in molecular oncology. In this study, we developed a continuous flow immunoassay device based on a piezoelectric (PZ) quartz crystal biosensor fabricated with whole-electrode occupation for the quantitative molecular diagnosis of tumor markers such as alpha-fetoprotein (AFP). Only one face of the crystal was in contact with the serum sample during the assays. First, the characteristics of AFP and anti-AFP binding kinetics, such as the optimal time for immune response, the average antigen binding rate, the kinetic constants and the optimal standard curve, were investigated. The overall immunoreaction time was only 12 min, the average antigen binding rate of AFP was 45.9 ng/min, the concentration range of AFP detection was 18.8-1100 ng/mL and the association rate constant (kon), dissociation rate constant (koff) and equilibrium dissociation constant (K
Identifiants
pubmed: 37887110
pii: bios13100917
doi: 10.3390/bios13100917
pmc: PMC10605586
pii:
doi:
Substances chimiques
Biomarkers, Tumor
0
alpha-Fetoproteins
0
Quartz
14808-60-7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : the research and development fund of southwest university of science and technology
ID : B1020140048
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