Ischemia-Reperfusion Injury in Porcine Aortic Valvular Endothelial and Interstitial Cells.
congenital cardiac surgery
ischemia reperfusion injury
partial heart transplantation
pediatric cardiac surgery
porcine
valve dysfunction
valvular endothelial cells
valvular interstitial cells
Journal
Journal of cardiovascular development and disease
ISSN: 2308-3425
Titre abrégé: J Cardiovasc Dev Dis
Pays: Switzerland
ID NLM: 101651414
Informations de publication
Date de publication:
19 Oct 2023
19 Oct 2023
Historique:
received:
16
08
2023
revised:
11
10
2023
accepted:
18
10
2023
medline:
27
10
2023
pubmed:
27
10
2023
entrez:
27
10
2023
Statut:
epublish
Résumé
Ischemia-reperfusion injury (IRI) in the myocardium has been thoroughly researched, especially in acute coronary syndrome and heart transplantation. However, our understanding of IRI implications on cardiac valves is still developing. This knowledge gap becomes even more pronounced given the advent of partial heart transplantation, a procedure designed to implant isolated human heart valves in young patients. This study aims to investigate the effects of IRI on aortic valvular endothelial cells (VECs), valvular interstitial cells (VICs), and whole leaflet cultures (no separation of VECs and VICs). We employed two conditions: hypoxic cold storage reperfusion (HCSR) and normothermia (NT). Key markers, secreted protein acidic and cysteine rich (SPARC) (osteonectin), and inducible nitric oxide synthase (iNOS2) were evaluated. In the isolated cells under HCSR, VICs manifested a significant 15-fold elevation in SPARC expression compared to NT (
Identifiants
pubmed: 37887883
pii: jcdd10100436
doi: 10.3390/jcdd10100436
pmc: PMC10607149
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NHLBI NIH HHS
ID : R41 HL169059
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001450
Pays : United States
Références
Innovations (Phila). 2023 Mar-Apr;18(2):126-131
pubmed: 36872577
Front Immunol. 2021 Aug 10;12:731361
pubmed: 34447390
Acta Cardiol. 2021 Jun;76(4):335-348
pubmed: 32602774
Cardiol Young. 2023 Aug;33(8):1401-1408
pubmed: 35968848
PLoS One. 2023 Feb 7;18(2):e0280163
pubmed: 36749770
J Thorac Cardiovasc Surg. 1998 Jan;115(1):118-27
pubmed: 9451054
J Card Surg. 2022 Dec;37(12):5307-5312
pubmed: 36259737
Surgery. 2021 Apr;169(4):983-985
pubmed: 32948337
Atherosclerosis. 2015 Sep;242(1):251-260
pubmed: 26232165
J Thorac Cardiovasc Surg. 1987 Dec;94(6):812-23
pubmed: 3682851
J Vis Exp. 2021 Sep 21;(175):
pubmed: 34633389
J Cardiovasc Dev Dis. 2023 May 26;10(6):
pubmed: 37367399
J Heart Lung Transplant. 2022 Oct;41(10):1357-1365
pubmed: 35989143
Ann Thorac Surg. 1995 Aug;60(2 Suppl):S135-40
pubmed: 7646145
J Cardiovasc Dev Dis. 2023 Jun 10;10(6):
pubmed: 37367419
J Cardiovasc Dev Dis. 2023 Mar 31;10(4):
pubmed: 37103027
Front Surg. 2021 Jul 13;8:676739
pubmed: 34327211
Ann Thorac Surg. 2003 Apr;75(4):1274-82
pubmed: 12683575
Cell Biochem Biophys. 2021 Dec;79(4):695-709
pubmed: 34661855