Non-touch treatment to solve the persistent contamination of culture plates with Aspergillus niger in a clinical microbiology laboratory of Spain.
Aspergillus niger
Disinfection/methods
Hydrogen peroxide
Laboratories, hospital
Peracetic acid
Journal
Environmental science and pollution research international
ISSN: 1614-7499
Titre abrégé: Environ Sci Pollut Res Int
Pays: Germany
ID NLM: 9441769
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
25
06
2023
accepted:
09
10
2023
medline:
29
11
2023
pubmed:
27
10
2023
entrez:
27
10
2023
Statut:
ppublish
Résumé
A pseudo-outbreak of Aspergillus caused by false positive cultures can have a high sanitary impact. We determined the effectiveness (fungal load elimination) of a non-touch disinfection system, vs conventional disinfection methods, to solve steady contamination of culture plates with Aspergillus niger at a clinical microbiology laboratory. Routine cleaning-disinfection (RCD), intensive cleaning-disinfection (ICD), and terminal airborne disinfection (TAD) were employed in stages. Air sampling was carried out before and after each procedure. The effectiveness of TAD on contact surfaces was tested by surface sampling. After RCD, ICD, and TAD, there was a mean decrease of 5.4 (95% CI = 1.8-9.0), 19.2 (95% CI = 12.4-26.0), and 4.4 (95% CI = 2.5-6.3) CFU per tested area, and 46.2%, 21.7%, and 95.5% of contaminated areas became sterile, respectively. There was a mean decrease of 30.6 CFU per tested surface (p < 0.0007) and 50% of tested surfaces became sterile. Global effectiveness of RCD, ICD, and TAD was 68.8% (95% CI = 68.5-69.1), 82.2% (95% CI = 82.1-82.3), and 99.0% (95% CI = 98.8-99.2), respectively. The effectiveness was higher with TAD (4.1 CFU/cm
Identifiants
pubmed: 37889415
doi: 10.1007/s11356-023-30433-8
pii: 10.1007/s11356-023-30433-8
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115734-115744Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Références
Amodio E, Kuster SP, Garzoni C, Zinkernagel AS, Sax H, Aline Wolfensberger A (2020) Disinfecting noncritical medical equipment — effectiveness of hydrogen peroxide dry mist as an adjunctive method. Am J Infect Control 48(8):897–902. https://doi.org/10.1016/j.ajic.2020.05.016
doi: 10.1016/j.ajic.2020.05.016
Andersen BM, Rasch M, Hochlin K, Jensen FH, Wismar P, Fredriksen JE (2006) Decontamination of rooms, medical equipment and ambulances using an aerosol of hydrogen peroxide disinfectant. J Hosp Infect 62(2):149–155. https://doi.org/10.1016/j.jhin.2005.07.020
doi: 10.1016/j.jhin.2005.07.020
Boyce JM, Havill NL, Cianci V, Flanagan G (2014) Compatibility of hydrogen peroxide vapor room decontamination with physiological monitors. Infect Control Hosp Epidemiol 35(1):92–93. https://doi.org/10.1086/674388
doi: 10.1086/674388
Carling PC, Parry MF, Bruno-Murtha LA, Dick B (2010) Improving environmental hygiene in 27 intensive care units to decrease multidrug-resistant bacterial transmission. Crit Care 38:1054–1059. https://doi.org/10.1097/CCM.0b013e3181cdf705
doi: 10.1097/CCM.0b013e3181cdf705
Carling PC, Parry MF, Von Beheren SM, Healthcare Environmental Hygiene Study Group (2008) Identifying opportunities to enhance environmental cleaning in 23 acute care hospitals. Infect Control Hosp Epidemiol 29:1–7. https://doi.org/10.1086/524329
doi: 10.1086/524329
Carling PC, Perkins J, Ferguson J, Thomasser A (2014) Evaluating a new paradigm for comparing surface disinfection in clinical practice. Infect Control Hosp Epidemiol 35(11):1349–1355. https://doi.org/10.1086/678424
doi: 10.1086/678424
Chang CC, Athan E, Morrissey CO, Slavin MA (2008) Preventing invasive fungal infection during hospital building works. Intern Med J 38(6b):538–541. https://doi.org/10.1111/j.1445-5994.2008.01727.x
doi: 10.1111/j.1445-5994.2008.01727.x
Collado C (2014) Aeroturbex. Jose Collado S.A. https://www.youtube.com/watch?v=7prtdeE9gTQ . Accessed 14 July 2022
Cunha BA, Klein NC (1995) Pseudoinfections. Infect Dis Clin Prac 4(2):95–103
doi: 10.1097/00019048-199503000-00004
Dancer SJ (2004) How do we assess hospital cleaning? A proposal for microbiological standards for surface hygiene in hospitals. J Hosp Infect 56:10–15. https://doi.org/10.1016/j.jhin.2003.09.017
doi: 10.1016/j.jhin.2003.09.017
Dancer SJ (2008) Importance of the environment in methicillin-resistant Staphylococcus aureus acquisition: the case for hospital cleaning. Lancet Infect Dis 8:101–113. https://doi.org/10.1016/S1473-3099(07)70241-4
doi: 10.1016/S1473-3099(07)70241-4
Doll M, Morgan DJ, Anderson D, Bearman G (2015) Touchless technologies for decontamination in the hospital: a review of hydrogen peroxide and UV devices. Curr Infect Dis Rep 17(9):498. https://doi.org/10.1007/s11908-015-0498-1
doi: 10.1007/s11908-015-0498-1
Eckstein BC, Adams DA, Eckstein EC, Rao A, Sethi AK, Yadavalli GK et al (2007) Reduction of Clostridium difficile and vancomycin-resistant Enterococcus contamination of environmental surfaces after an intervention to improve cleaning methods. BMC Infect Dis 7:61. https://doi.org/10.1186/1471-2334-7-61
doi: 10.1186/1471-2334-7-61
Falagas ME, Thomaidis PC, Kotsantis IK, Sgouros K, Samonis G, Karageorgopoulos DE (2011) Airborne hydrogen peroxide for disinfection of the hospital environment and infection control: a systematic review. J Hosp Infect 78(3):171–177. https://doi.org/10.1016/j.jhin.2010.12.006
doi: 10.1016/j.jhin.2010.12.006
Hacek DM, Ogle AM, Fisher A, Robicsek A, Peterson LR (2010) Significant impact of terminal room cleaning with bleach on reducing nosocomial Clostridium difficile. Am J Infect Control 38:350–353. https://doi.org/10.1016/j.ajic.2009.11.003
doi: 10.1016/j.ajic.2009.11.003
Hayden MK, Bonten MJM, Blom DW, Lyle EA, van de Vijver DA, Weinstein RA (2006) Reduction in acquisition of vancomycin-resistant enterococcus after enforcement of routine environmental cleaning measures. Clin Infect Dis 42:1552–1560. https://doi.org/10.1086/503845
doi: 10.1086/503845
Kanamori H, Rutala WA, Sickbert-Bennett EE, Weber DJ (2015) Review of fungal outbreaks and infection prevention in healthcare settings during construction and renovation. Clin Infect Dis 61(3):433–444. https://doi.org/10.1093/cid/civ297
doi: 10.1093/cid/civ297
Laurel VL, Meier PA, Astorga A, Dolan D, Brockett R, Rinaldi MG (1999) Pseudoepidemic of Aspergillus niger infections traced to specimen contamination in the microbiology laboratory. J Clin Microbiol 37(5):1612–1616. https://doi.org/10.1128/JCM.37.5.1612-1616.1999
doi: 10.1128/JCM.37.5.1612-1616.1999
Liofilchem® - Contact Slide 2- Rev.3 / 04.05.2017. Instructions for use. http://www.liofilchem.net/login/pd/ifu/525272_IFU.pdf . Accessed 17 May 2019
McClenny N (2005) Laboratory detection and identification of Aspergillus species by microscopic observation and culture: the traditional approach. Med Mycol 43(Suppl 1):S125–S128. https://doi.org/10.1080/13693780500052222
doi: 10.1080/13693780500052222
Orlando P, Cristina ML, Dallera M, Ottria G, Vitale A, Badolati G (2008) Surface disinfection: evaluation of the efficacy of a nebulization system spraying hydrogen peroxide. J Prev Med Hyg 49(3):116–119
Pyrgiotakis G, McDevitt J, Bordini A, Diaz E, Molina R, Watson C, et al (2014) A chemical free, nanotechnology-based method for airborne bacterial inactivation using engineered water nanostructures. Environ Sci Nano 15–26. https://doi.org/10.1039/C3EN00007A
Quinn MM, Henneberger PK, Braun B, Delclos GL, Fagan K, Huang V et al (2015) Cleaning and disinfecting environmental surfaces in health care: toward an integrated framework for infection and occupational illness prevention. Am J Infect Control 43(5):424–434. https://doi.org/10.1016/j.ajic.2015.01.029
doi: 10.1016/j.ajic.2015.01.029
Rutala WA, Weber DJ, and the Healthcare Infection Control Practices Advisory Committee (HICPAC) (2008) Guideline for disinfection and sterilization in healthcare facilities. http://www.cdc.gov/hicpac/pdf/guidelines/Disinfection_Nov_2008.pdf . Accessed 17 January 2022
Sanguinet J, Edmiston Ch (2021) Evaluation of dry hydrogen peroxide in reducing microbial bioburden in a healthcare facility. Am J Infect Control 49(8):985–990. https://doi.org/10.1016/j.ajic.2021.03.004
doi: 10.1016/j.ajic.2021.03.004
Sitzlar B, Deshpande A, Fertelli D, Kundrapu S, Sethi AK, Donskey CJ (2013) An environmental disinfection odyssey: evaluation of sequential interventions to improve disinfection of Clostridium difficile isolation rooms. Infect Control Hosp Epidemiol 34:459–465. https://doi.org/10.1086/670217
doi: 10.1086/670217
Spaulding EH (1972) Chemical disinfection and antisepsis in the hospital. J Hosp Res 9:5–31
Theilen U, Wilsberg FJ, Böhm R, Strauch D (1987) Aerosol disinfection of bacterial spores. Zentralbl Bakteriol Mikrobiol Hyg B Umwelthyg Krankenhaushyg Arbeitshyg Prav Med 184(3–4):229–252
Vaze N, Jiang Y, Mena L, Zhang Y, Bello D, Leonard S et al (2018) An integrated electrolysis-electrospray-ionization antimicrobial platform using engineered water nanostructures (EWNS) for food safety applications. Food Control 85:151–160. https://doi.org/10.1016/j.foodcont.2017.09.034
doi: 10.1016/j.foodcont.2017.09.034