HSP60 mimetic peptides from Mycobacterium leprae as new antigens for immunodiagnosis of Leprosy.
Chaperonin
IgG antibody
Lepromatous patients
Phage display
Journal
AMB Express
ISSN: 2191-0855
Titre abrégé: AMB Express
Pays: Germany
ID NLM: 101561785
Informations de publication
Date de publication:
27 Oct 2023
27 Oct 2023
Historique:
received:
15
03
2023
accepted:
09
10
2023
medline:
28
10
2023
pubmed:
28
10
2023
entrez:
27
10
2023
Statut:
epublish
Résumé
The early diagnosis of leprosy serves as an important tool to reduce the incidence of this disease in the world. Phage display (PD) technology can be used for mapping new antigens to the development of immunodiagnostic platforms. Our objective was to identify peptides that mimic Mycobacterium leprae proteins as serological markers using phage display technology. The phages were obtained in the biopanning using negative and positive serum from household contacts and leprosy patients, respectively. Then, the peptides were synthesized and validated in silico and in vitro for detection of IgG from patients and contacts. To characterize the native protein of M. leprae, scFv antibodies were selected against the synthetic peptides by PD. The scFv binding protein was obtained by immunocapture and confirmed using mass spectrometry. We selected two phase-fused peptides, MPML12 and MPML14, which mimic the HSP60 protein from M. leprae. The peptides MPML12 and MPML14 obtained 100% and 92.85% positivity in lepromatous patients. MPML12 and MPM14 detect IgG, especially in the multibacillary forms. The MPML12 and MPML14 peptides had positivity of 11.1% and 16.6% in household contacts, respectively. There was no cross-reaction in patient's samples with visceral leishmaniasis, tuberculosis and other mycobacteriosis for both peptides. Given these results and the easy obtainment of mimetic antigens, our peptides are promising markers for application in the diagnosis of leprosy, especially in endemic and hyperendemic regions.
Identifiants
pubmed: 37891336
doi: 10.1186/s13568-023-01625-9
pii: 10.1186/s13568-023-01625-9
pmc: PMC10611693
doi:
Types de publication
Journal Article
Langues
eng
Pagination
120Subventions
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : CNPq-465669/2014-0
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : finance code 001
Organisme : Fundação de Amparo à Pesquisa do Estado de Minas Gerais
ID : FAPEMIG-CBB-APQ-03613-17
Organisme : Fundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão
ID : PPSUS-05972/16
Organisme : Fundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão
ID : UNIVERSAL-01015/16
Informations de copyright
© 2023. The Author(s).
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