Expression of dengue virus and Zika virus NS2B-NS3pro constructs alter cellular fatty acids, but co-expression with a Zika virus virus-like particle is detrimental to virus-like particle expression.
Dengue virus
Fatty acid methyl esters
Lipid
NS3 protease
Virus-like particles
Zika virus
Journal
BMC research notes
ISSN: 1756-0500
Titre abrégé: BMC Res Notes
Pays: England
ID NLM: 101462768
Informations de publication
Date de publication:
27 Oct 2023
27 Oct 2023
Historique:
received:
07
11
2022
accepted:
12
10
2023
medline:
30
10
2023
pubmed:
28
10
2023
entrez:
27
10
2023
Statut:
epublish
Résumé
Studies have shown that Flavivirus infection remodels the host cell to favour viral replication. In particular, the host cell lipid profile is altered, and it has been proposed that this process alters membrane fluidity to allow wrapping of the outer structural proteins around the viral nucleocapsid. We investigated whether expression of the Zika virus (ZIKV) and dengue virus (DENV) protease induced alterations in the cellular lipid profile, and subsequently whether co-expression of these proteases with VLP constructs was able to improve VLP yield. Our results showed that both ZIKV and DENV proteases induced alterations in the lipid profile, but that both active and inactive proteases induced many of the same changes. Neither co-transfection of protease and VLP constructs nor bicistronic vectors allowing expression of both protease and VLP separated by a cell cleavable linker improved VLP yield, and indeed many of the constructs showed significantly reduced VLP production. Further work in developing improved VLP expression platforms is required.
Identifiants
pubmed: 37891687
doi: 10.1186/s13104-023-06572-z
pii: 10.1186/s13104-023-06572-z
pmc: PMC10605870
doi:
Substances chimiques
Viral Nonstructural Proteins
0
Peptide Hydrolases
EC 3.4.-
Lipids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
296Subventions
Organisme : Thailand Research Fund
ID : IRN5802PHDW03 under IRN58W0002
Organisme : Thailand Research Fund
ID : IRN5802PHDW03 under IRN58W0002
Organisme : National Research Council of Thailand
ID : NRCT5-RGJ63012-139
Organisme : Thailand Graduate Institute of Science and Technology
ID : TG-22-14-59-005D
Organisme : National Science and Technology Development Agency
ID : FDA-CO-2561-6820-TH
Organisme : Mahidol University
ID : BRF1-088/2565
Informations de copyright
© 2023. The Author(s).
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