The Study of Performance of a Nanoribbon Biosensor, Sensitized with Aptamers and Antibodies, upon Detection of Core Antigen of Hepatitis C Virus.
HCVcoreAg
antibody
aptamer
diagnostics
nanoribbon biosensor
Journal
Micromachines
ISSN: 2072-666X
Titre abrégé: Micromachines (Basel)
Pays: Switzerland
ID NLM: 101640903
Informations de publication
Date de publication:
19 Oct 2023
19 Oct 2023
Historique:
received:
31
08
2023
revised:
09
10
2023
accepted:
11
10
2023
medline:
28
10
2023
pubmed:
28
10
2023
entrez:
28
10
2023
Statut:
epublish
Résumé
The development of highly sensitive diagnostic systems for the early revelation of diseases in humans is one of the most important tasks of modern biomedical research, and the detection of the core antigen of the hepatitis C virus (HCVcoreAg)-a protein marker of the hepatitis C virus-is just the case. Our study is aimed at testing the performance of the nanoribbon biosensor in the case of the use of two different types of molecular probes: the antibodies and the aptamers against HCVcoreAg. The nanoribbon sensor chips employed are based on "silicon-on-insulator structures" (SOI-NR). Two different HCVcoreAg preparations are tested: recombinant β-galactosidase-conjugated HCVcoreAg ("Virogen", Watertown, MA, USA) and recombinant HCVcoreAg ("Vector-Best", Novosibirsk, Russia). Upon the detection of either type of antigen preparation, the lowest concentration of the antigen detectable in buffer with pH 5.1 was found to be approximately equal, amounting to ~10
Identifiants
pubmed: 37893383
pii: mi14101946
doi: 10.3390/mi14101946
pmc: PMC10609547
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Program for Basic Research in the Russian Federation for a long-term period (2021-2030)
ID : 122030100168-2
Organisme : The Ministry of Science and Higher Education of the Russian Federation
ID : 075-01129-23-00
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