Enzymatic Cleavage of Stx2a in the Gut and Identification of Pancreatic Elastase and Trypsin as Possible Main Cleavers.

EHEC-associated hemolytic uremic syndrome (eHUS) Shiga toxin 2a (Stx2a) chymotrypsin-like elastase 3B (CELA3B) enterohemorrhagic Escherichia coli (EHEC) furin trypsin

Journal

Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893

Informations de publication

Date de publication:
04 Oct 2023
Historique:
received: 06 07 2023
revised: 15 09 2023
accepted: 02 10 2023
medline: 28 10 2023
pubmed: 28 10 2023
entrez: 28 10 2023
Statut: epublish

Résumé

Shiga toxins (Stxs), especially the Stx2a subtype, are the major virulence factors involved in enterohemorrhagic

Identifiants

pubmed: 37894145
pii: microorganisms11102487
doi: 10.3390/microorganisms11102487
pmc: PMC10609011
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : FWF Austrian Science Fund
ID : W1253
Organisme : European Union
ID : 860044

Références

Am J Physiol Gastrointest Liver Physiol. 2009 Jan;296(1):G78-92
pubmed: 18974311
J Immunol. 2009 May 15;182(10):6394-400
pubmed: 19414792
Cell Microbiol. 2019 May;21(5):e13000
pubmed: 30578712
Lab Med. 2016 May;47(2):140-8
pubmed: 27069032
Hum Genet. 2021 May;140(5):747-760
pubmed: 33221945
Mol Microbiol. 2002 Jan;43(1):207-15
pubmed: 11849548
Clin Lab. 2011;57(11-12):993-8
pubmed: 22239032
Infect Immun. 2010 Nov;78(11):4488-99
pubmed: 20732996
Lancet. 2005 Mar 19-25;365(9464):1073-86
pubmed: 15781103
Microbiol Rev. 1987 Jun;51(2):206-20
pubmed: 3299029
J Nutr. 2000 May;130(5S Suppl):1437S-46S
pubmed: 10801957
Front Cell Infect Microbiol. 2022 Feb 10;12:825856
pubmed: 35223548
Int J Med Microbiol. 2005 Oct;295(6-7):405-18
pubmed: 16238016
Arch Biochem Biophys. 1981 Oct 1;211(1):253-7
pubmed: 7030213
J Infect Dis. 2003 Jan 1;187(1):154-8
pubmed: 12508161
Diagn Microbiol Infect Dis. 2007 Nov;59(3):235-42
pubmed: 17931818
Clin Transl Immunology. 2019 Aug 05;8(8):e1073
pubmed: 31406574
Am J Physiol Gastrointest Liver Physiol. 2017 Jun 1;312(6):G606-G614
pubmed: 28360028
Exp Cell Res. 1995 May;218(1):39-49
pubmed: 7737376
J Biol Chem. 1995 May 5;270(18):10817-21
pubmed: 7738018
Int J Med Microbiol. 2018 Dec;308(8):1073-1084
pubmed: 30224239
Biochem Biophys Res Commun. 2007 May 25;357(1):144-9
pubmed: 17407762
J Biol Chem. 2000 Feb 4;275(5):3713-21
pubmed: 10652371
Infect Immun. 1999 Dec;67(12):6670-7
pubmed: 10569789
MMWR Recomm Rep. 2009 Oct 16;58(RR-12):1-14
pubmed: 19834454
Infect Immun. 1996 May;64(5):1569-76
pubmed: 8613362
Front Cell Infect Microbiol. 2020 Feb 04;10:21
pubmed: 32117798
Toxins (Basel). 2011 Jun;3(6):626-39
pubmed: 22069729
Microbiology (Reading). 1999 May;145 ( Pt 5):999-1004
pubmed: 10376814
Pediatr Nephrol. 2016 Nov;31(11):2163-70
pubmed: 27240858
J Biol Chem. 1981 Aug 25;256(16):8732-8
pubmed: 7021550
Cell Mol Gastroenterol Hepatol. 2020;10(1):171-190
pubmed: 32145469
Infect Immun. 1996 Aug;64(8):3294-300
pubmed: 8757867
Can J Gastroenterol. 2013;27(5):281-5
pubmed: 23712303
J Infect Dis. 1990 Aug;162(2):553-6
pubmed: 2197346
Clin Infect Dis. 2012 May;54(10):1413-21
pubmed: 22412065
Nanomaterials (Basel). 2019 Jun 20;9(6):
pubmed: 31226785
PLoS One. 2014 Mar 26;9(3):e93463
pubmed: 24671194
Infect Immun. 2011 Sep;79(9):3527-40
pubmed: 21708996
Biomolecules. 2023 Feb 06;13(2):
pubmed: 36830674
Glycobiology. 2012 Jun;22(6):849-62
pubmed: 22279060

Auteurs

Sára Kellnerová (S)

Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Silke Huber (S)

Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Mariam Massri (M)

Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Verena Fleischer (V)

Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Klemens Losso (K)

Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck, 6020 Innsbruck, Austria.
Department of Food Technology and Nutrition, MCI|The Entrepreneurial School, 6020 Innsbruck, Austria.

Bettina Sarg (B)

Protein Core Facility, Institute of Medical Biochemistry, Center of Chemistry and Biomedicine, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Leopold Kremser (L)

Protein Core Facility, Institute of Medical Biochemistry, Center of Chemistry and Biomedicine, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Heribert Talasz (H)

Protein Core Facility, Institute of Medical Biochemistry, Center of Chemistry and Biomedicine, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Xiaohua He (X)

Western Regional Research Center, U.S. Department of Agriculture, Agricultural Research Service, Albany, CA 74710, USA.

Elisa Varrone (E)

Department of Medical and Surgical Sciences, School of Medicine, University of Bologna, 40126 Bologna, Italy.

Maurizio Brigotti (M)

Department of Medical and Surgical Sciences, School of Medicine, University of Bologna, 40126 Bologna, Italy.

Gianluigi Ardissino (G)

Center for HUS Prevention, Control and Management at Pediatric Nephrology, Dialysis and Transplant Unit, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 20122 Milan, Italy.

Dorothea Orth-Höller (D)

Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, 6020 Innsbruck, Austria.
MB-LAB-Clinical Microbiology Laboratory, 6020 Innsbruck, Austria.

Reinhard Würzner (R)

Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Classifications MeSH