Short-Term Inhibition of Translation by Cycloheximide Concurrently Affects Mitochondrial Function and Insulin Secretion in Islets from Female Mice.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
23 Oct 2023
Historique:
received: 15 09 2023
revised: 10 10 2023
accepted: 18 10 2023
medline: 30 10 2023
pubmed: 28 10 2023
entrez: 28 10 2023
Statut: epublish

Résumé

Since glucose stimulates protein biosynthesis in beta cells concomitantly with the stimulation of insulin release, the possible interaction of both processes was explored. The protein biosynthesis was inhibited by 10 μM cycloheximide (CHX) 60 min prior to the stimulation of perifused, freshly isolated or 22 h-cultured NMRI mouse islets. CHX reduced the insulinotropic effect of 25 mM glucose or 500 μM tolbutamide in fresh but not in cultured islets. In cultured islets the second phase of glucose stimulation was even enhanced. In fresh and in cultured islets CHX strongly reduced the content of proinsulin, but not of insulin, and moderately diminished the [Ca

Identifiants

pubmed: 37895141
pii: ijms242015464
doi: 10.3390/ijms242015464
pmc: PMC10607510
pii:
doi:

Substances chimiques

Cycloheximide 98600C0908
Insulin 0
Glucose IY9XDZ35W2
Tolbutamide 982XCM1FOI
NAD 0U46U6E8UK
Calcium SY7Q814VUP

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Deutsche Forschungsgemeinschaft
ID : Ru 368/5-4
Organisme : Deutsche Diabetes Gesellschaft
ID : n/a

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Auteurs

Mohammed Alshafei (M)

Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, D38106 Braunschweig, Germany.

Torben Schulze (T)

Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, D38106 Braunschweig, Germany.

Mai Morsi (M)

Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, D38106 Braunschweig, Germany.
Department of Pharmacology, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.

Uwe Panten (U)

Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, D38106 Braunschweig, Germany.

Ingo Rustenbeck (I)

Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, D38106 Braunschweig, Germany.

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Classifications MeSH