Antiviral responses in a Jamaican fruit bat intestinal organoid model of SARS-CoV-2 infection.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
28 10 2023
28 10 2023
Historique:
received:
03
12
2022
accepted:
16
10
2023
medline:
30
10
2023
pubmed:
29
10
2023
entrez:
29
10
2023
Statut:
epublish
Résumé
Bats are natural reservoirs for several zoonotic viruses, potentially due to an enhanced capacity to control viral infection. However, the mechanisms of antiviral responses in bats are poorly defined. Here we established a Jamaican fruit bat (JFB, Artibeus jamaicensis) intestinal organoid model of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Upon infection with SARS-CoV-2, increased viral RNA and subgenomic RNA was detected, but no infectious virus was released, indicating that JFB organoids support only limited viral replication but not viral reproduction. SARS-CoV-2 replication was associated with significantly increased gene expression of type I interferons and inflammatory cytokines. Interestingly, SARS-CoV-2 also caused enhanced formation and growth of JFB organoids. Proteomics revealed an increase in inflammatory signaling, cell turnover, cell repair, and SARS-CoV-2 infection pathways. Collectively, our findings suggest that primary JFB intestinal epithelial cells mount successful antiviral interferon responses and that SARS-CoV-2 infection in JFB cells induces protective regenerative pathways.
Identifiants
pubmed: 37898615
doi: 10.1038/s41467-023-42610-x
pii: 10.1038/s41467-023-42610-x
pmc: PMC10613288
doi:
Substances chimiques
Interferon Type I
0
Antiviral Agents
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6882Subventions
Organisme : NIAID NIH HHS
ID : R01 AI140442
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI169536
Pays : United States
Organisme : NIGMS NIH HHS
ID : R24 GM137786
Pays : United States
Organisme : NIBIB NIH HHS
ID : U01 EB029242
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2023. The Author(s).
Références
Nature. 2020 Mar;579(7798):270-273
pubmed: 32015507
Lancet Microbe. 2020 Sep;1(5):e218-e225
pubmed: 32838346
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
Cell Rep. 2020 Nov 3;33(5):108345
pubmed: 33147460
Cell Rep. 2020 Jul 7;32(1):107863
pubmed: 32610043
Patterns (N Y). 2020 Sep 11;1(6):100090
pubmed: 32838343
PLoS Biol. 2022 Mar 31;20(3):e3001592
pubmed: 35358182
Dev Comp Immunol. 2018 Aug;85:95-107
pubmed: 29635006
Cold Spring Harb Perspect Biol. 2018 Nov 1;10(11):
pubmed: 28963110
Mol Cell Proteomics. 2014 Feb;13(2):397-406
pubmed: 24309898
Viruses. 2014 Dec;6(12):4880-901
pubmed: 25494448
Emerg Infect Dis. 2010 Aug;16(8):1217-23
pubmed: 20678314
Front Immunol. 2020 Dec 11;11:608645
pubmed: 33362795
J Immunol. 2018 Jan 1;200(1):209-217
pubmed: 29180486
mSphere. 2023 Feb 21;8(1):e0026322
pubmed: 36598226
BMC Bioinformatics. 2013 Apr 15;14:128
pubmed: 23586463
Nature. 2020 Oct;586(7830):509-515
pubmed: 32967005
J Wildl Dis. 2013 Jul;49(3):684-9
pubmed: 23778622
Elife. 2018 Dec 06;7:
pubmed: 30520725
Emerg Microbes Infect. 2023 Dec;12(1):e2148561
pubmed: 36440480
Nature. 2021 Mar;591(7849):293-299
pubmed: 33494095
PLoS Negl Trop Dis. 2019 Feb 4;13(2):e0007071
pubmed: 30716104
Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2696-701
pubmed: 26903655
Front Immunol. 2017 Sep 11;8:1098
pubmed: 28959255
Cell. 2016 Jun 16;165(7):1586-1597
pubmed: 27315476
Sci Immunol. 2020 May 13;5(47):
pubmed: 32404436
Nat Protoc. 2013 Dec;8(12):2471-82
pubmed: 24232249
Nucleic Acids Res. 2014 Jan;42(Database issue):D756-63
pubmed: 24259432
Cell Tissue Res. 2018 Feb;371(2):293-307
pubmed: 29178040
Nature. 2005 Dec 1;438(7068):575-6
pubmed: 16319873
Viruses. 2022 Aug 18;14(8):
pubmed: 36016431
ACS Omega. 2020 Sep 30;5(40):25625-25633
pubmed: 33073088
Nucleic Acids Res. 2015 Apr 20;43(7):e47
pubmed: 25605792
FASEB J. 2008 Jul;22(7):2243-52
pubmed: 18263700
Viruses. 2021 Mar 26;13(4):
pubmed: 33810401
Elife. 2020 Feb 03;9:
pubmed: 32011232
Nature. 2020 May;581(7809):465-469
pubmed: 32235945
Bioinformatics. 2014 Feb 15;30(4):523-30
pubmed: 24336805
Viruses. 2019 Feb 23;11(2):
pubmed: 30813403
Sci Rep. 2018 Mar 28;8(1):5319
pubmed: 29593296
Front Microbiol. 2021 Oct 28;12:746795
pubmed: 34777295
Front Cell Infect Microbiol. 2020 Dec 11;10:527921
pubmed: 33363045
Emerg Infect Dis. 2013 Nov;19(11):1819-23
pubmed: 24206838
Cell Host Microbe. 2018 Mar 14;23(3):297-301.e4
pubmed: 29478775
Cell Mol Gastroenterol Hepatol. 2019;8(1):157-171.e3
pubmed: 30878664
Nat Ecol Evol. 2021 May;5(5):600-608
pubmed: 33649547
PLoS Pathog. 2020 Dec 7;16(12):e1009130
pubmed: 33284849
Vaccines (Basel). 2020 May 17;8(2):
pubmed: 32429501
Nature. 2021 Sep;597(7878):603
pubmed: 34561634
J Proteome Res. 2021 May 7;20(5):2547-2559
pubmed: 33840197
Nat Med. 2020 Jul;26(7):1077-1083
pubmed: 32405028
J Am Assoc Lab Anim Sci. 2020 May 1;59(3):275-281
pubmed: 32164795
Cell. 2020 Aug 6;182(3):685-712.e19
pubmed: 32645325
J Virol. 2015 Oct 07;90(1):43-56
pubmed: 26446608
Biol Open. 2017 May 15;6(5):698-705
pubmed: 28347989
Aliment Pharmacol Ther. 2017 Nov;46(9):800-815
pubmed: 28869283
J Immunol. 2011 Mar 1;186(5):3138-47
pubmed: 21278349
J Virol. 2022 Jul 27;96(14):e0060822
pubmed: 35862713
Mol Syst Biol. 2021 Apr;17(4):e10232
pubmed: 33904651
PLoS Pathog. 2013;9(10):e1003657
pubmed: 24130481
Nat Rev Nephrol. 2015 Sep;11(9):546-54
pubmed: 26215513
Nat Commun. 2023 Oct 28;14(1):6882
pubmed: 37898615
PLoS Pathog. 2011 Oct;7(10):e1002315
pubmed: 22028656
Nat Microbiol. 2023 Jun;8(6):1176-1186
pubmed: 37231088
Cell Death Dis. 2021 Jan 18;12(1):95
pubmed: 33462215
PLoS One. 2012;7(11):e48472
pubmed: 23166587
Int J Mol Sci. 2021 Oct 05;22(19):
pubmed: 34639103
Nat Methods. 2019 Jun;16(6):509-518
pubmed: 31133760
Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2058-E2067
pubmed: 29440426
Cell Mol Gastroenterol Hepatol. 2017 Dec 09;5(3):241-251
pubmed: 29675450
mSphere. 2017 Sep 27;2(5):
pubmed: 28959737
Mol Ecol. 2020 Nov;29(22):4366-4381
pubmed: 32633855
Front Immunol. 2022 Jun 10;13:918881
pubmed: 35757773
J Gen Virol. 2017 Sep;98(9):2297-2309
pubmed: 28840816
Cell. 2018 May 17;173(5):1098-1110.e18
pubmed: 29706541
Nat Commun. 2020 Jul 30;11(1):3810
pubmed: 32733001
Sci Rep. 2016 Feb 22;6:21878
pubmed: 26899616
Int Immunopharmacol. 2022 Aug;109:108822
pubmed: 35605524
Anal Chim Acta. 2017 Apr 29;964:7-23
pubmed: 28351641
Cell Mol Gastroenterol Hepatol. 2018 May 30;6(2):218-222.e7
pubmed: 30105282
Microorganisms. 2020 Mar 28;8(4):
pubmed: 32231141
Curr Opin Virol. 2019 Feb;34:79-89
pubmed: 30665189
Science. 2020 Jul 3;369(6499):50-54
pubmed: 32358202
Virus Res. 2018 Mar 15;248:5-12
pubmed: 29454637
Nat Genet. 2021 Apr;53(4):435-444
pubmed: 33686287
Proc Natl Acad Sci U S A. 2021 Mar 16;118(11):
pubmed: 33836570
PLoS Pathog. 2023 Oct 19;19(10):e1011728
pubmed: 37856551
Nature. 2021 Jan;589(7842):363-370
pubmed: 33473223
Nat Commun. 2018 Dec 3;9(1):5128
pubmed: 30510204
Transbound Emerg Dis. 2021 Nov;68(6):3443-3452
pubmed: 33295095
Mol Ecol. 2020 May;29(9):1745-1755
pubmed: 32279365
Viruses. 2014 Apr 17;6(4):1759-88
pubmed: 24747773
J Neurovirol. 2001 Dec;7(6):511-7
pubmed: 11704883
J Proteome Res. 2020 Sep 4;19(9):3595-3606
pubmed: 32786681
Nature. 2020 Oct;586(7827):113-119
pubmed: 32707573