Detection of bovine viral diarrhea virus and bovine herpes virus type 1 in cattle with and without endometritis.
Bovine herpes virus type 1
Bovine viral diarrhea virus
Cattle
Uterus
Journal
Veterinary research forum : an international quarterly journal
ISSN: 2008-8140
Titre abrégé: Vet Res Forum
Pays: Iran
ID NLM: 101625812
Informations de publication
Date de publication:
2023
2023
Historique:
received:
31
03
2023
accepted:
17
06
2023
medline:
30
10
2023
pubmed:
30
10
2023
entrez:
30
10
2023
Statut:
ppublish
Résumé
This study aimed to investigate the potential presence of bovine herpes virus type 1 (BHV-1) and bovine viral diarrhea virus (BVDV) in cattle uteri that did not display any clinical and macroscopic signs of infection. Virus detection involved polymerase chain reaction (PCR) test, double immunohistochemistry (IHC), and double immunofluorescence (IF). One hundred cornu uterus samples were collected from cattle aged 1 year and older. The BVDV was detected by PCR or by double IHC/IF in the collected samples from slaughterhouses in Kayseri city (Central Anatolia, Türkiye) from 2021 - 2022. By contrast, BHV-1 was detected by PCR and double IHC/IF at a rate of 16.00% and 21.00%, respectively. In the IHC and IF detection, BHV-1 was detected in endometrial epithelial cells and in some mononuclear cells in the lamina propria, periglandular areas and myometrium. Although no macroscopic lesion was found in the BHV-1-positive samples (n = 21), histopathological detection showed that two had acute endometritis, eight had subacute endometritis, eight had chronic endometritis and the three others showed no signs of endometritis. This prevalence study demonstrated for the first time that even while BVDV could not be detected in the samples, BHV-1 posed a critical potential reproductive risk in pregnant animals, as it can specifically cause abortions when it resides in cattle uteri that do not show clinical or macroscopic and even microscopic signs of infection. Additionally, this study was the first to combine PCR and double IHC/IF for BHV-1 and BVDV detection in cattle uteri.
Identifiants
pubmed: 37901355
doi: 10.30466/vrf.2023.1999091.3830
pmc: PMC10612390
doi:
Types de publication
Journal Article
Langues
eng
Pagination
541-548Informations de copyright
© 2023 Urmia University. All rights reserved.
Déclaration de conflit d'intérêts
There is no declared conflict of interest.
Références
J Virol Methods. 1993 Sep;44(1):129-39
pubmed: 8227276
Theriogenology. 2006 May;65(8):1516-30
pubmed: 16226305
J Virol. 1999 Oct;73(10):8657-68
pubmed: 10482619
Arch Virol. 2009;154(1):171-7
pubmed: 19066710
J Clin Microbiol. 1993 Dec;31(12):3129-35
pubmed: 8308103
Trop Anim Health Prod. 2020 Mar;52(2):555-564
pubmed: 31482481
J Virol. 2003 Apr;77(8):4848-57
pubmed: 12663791
J Dairy Sci. 2002 Sep;85(9):2223-36
pubmed: 12362455
Vet Rec. 1993 Jul 10;133(2):39-43
pubmed: 8212473
J Vet Diagn Invest. 1989 Jul;1(3):231-6
pubmed: 2562195
Am J Vet Res. 1986 Feb;47(2):223-8
pubmed: 2420240
Trop Anim Health Prod. 2019 Sep;51(7):1953-1961
pubmed: 31055737
J Vet Diagn Invest. 2006 Nov;18(6):600-5
pubmed: 17121092
Vet Clin North Am Food Anim Pract. 2010 Mar;26(1):105-21, table of contents
pubmed: 20117546
J Dairy Sci. 1999 May;82(5):944-52
pubmed: 10342232
Trop Anim Health Prod. 2018 Feb;50(2):353-363
pubmed: 29071668
Virology. 1995 Apr 20;208(2):565-75
pubmed: 7747428
Anim Health Res Rev. 2019 Jun;20(1):72-85
pubmed: 31895016
Theriogenology. 2013 Sep 15;80(5):451-5
pubmed: 23768649
Ir Vet J. 2013 Aug 01;66(1):15
pubmed: 23916092
J Vet Diagn Invest. 2012 Jul;24(4):662-70
pubmed: 22649159
Anim Reprod Sci. 2002 Jun 17;71(3-4):169-79
pubmed: 12047926
Arch Razi Inst. 2022 Oct 31;77(5):1621-1629
pubmed: 37123168
J Gen Virol. 1999 Jun;80 ( Pt 6):1477-1483
pubmed: 10374966
Vaccines (Basel). 2021 Jan 13;9(1):
pubmed: 33451136
Vet Microbiol. 1994 Sep;42(1):53-64
pubmed: 7839585
J Vet Med Sci. 2015 Nov;77(11):1371-7
pubmed: 26096964
Vet J. 2014 Feb;199(2):201-9
pubmed: 24053990
Theriogenology. 2019 May;130:125-129
pubmed: 30884333
Biologicals. 2003 Jun;31(2):107-12
pubmed: 12770540
Transbound Emerg Dis. 2012 Aug;59(4):303-10
pubmed: 22085446
Vet Microbiol. 1995 Jan;43(1):75-84
pubmed: 7536370
Infect Immun. 1982 Mar;35(3):1048-57
pubmed: 6175574
J Comp Pathol. 2013 Nov;149(4):391-401
pubmed: 23885803
Anim Health Res Rev. 2009 Jun;10(1):85-98
pubmed: 19558751