Microbial degradation and assimilation of veratric acid in oxic and anoxic groundwaters.
PLFA
SIP
assimilation
degradation
groundwater
heterotroph
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2023
2023
Historique:
received:
03
07
2023
accepted:
20
09
2023
medline:
30
10
2023
pubmed:
30
10
2023
entrez:
30
10
2023
Statut:
epublish
Résumé
Microbial communities are key players in groundwater ecosystems. In this dark environment, heterotrophic microbes rely on biomass produced by the activity of lithoautotrophs or on the degradation of organic matter seeping from the surface. Most studies on bacterial diversity in groundwater habitats are based on 16S gene sequencing and full genome reconstructions showing potential metabolic pathways used in these habitats. However, molecular-based studies do not allow for the assessment of population dynamics over time or the assimilation of specific compounds and their biochemical transformation by microbial communities. Therefore, in this study, we combined DNA-, phospholipid fatty acid-, and metabolomic-stable isotope probing to target and identify heterotrophic bacteria in the groundwater setting of the Hainich Critical Zone Exploratory (CZE), focusing on 2 aquifers with different physico-chemical conditions (oxic and anoxic). We incubated groundwater from 4 different wells using either
Identifiants
pubmed: 37901809
doi: 10.3389/fmicb.2023.1252498
pmc: PMC10602745
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1252498Informations de copyright
Copyright © 2023 Lazar, Schwab, Ueberschaar, Pohnert, Trumbore and Küsel.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Environ Microbiol. 2018 Jan;20(1):369-384
pubmed: 29194923
Can J Biochem Physiol. 1959 Aug;37(8):911-7
pubmed: 13671378
Microb Ecol. 2018 Nov;76(4):976-990
pubmed: 29728707
Appl Environ Microbiol. 2009 Dec;75(23):7537-41
pubmed: 19801464
Eur J Biochem. 1998 May 1;253(3):706-11
pubmed: 9654069
Genes (Basel). 2022 Dec 20;14(1):
pubmed: 36672742
Appl Environ Microbiol. 2000 Jun;66(6):2311-7
pubmed: 10831404
Proc Natl Acad Sci U S A. 2011 Mar 8;108(10):4170-5
pubmed: 21368116
ISME J. 2012 Sep;6(9):1665-76
pubmed: 22456444
Nat Protoc. 2007;2(4):860-6
pubmed: 17446886
J Basic Microbiol. 2009 Sep;49 Suppl 1:S98-102
pubmed: 19718680
Appl Environ Microbiol. 2015 Apr;81(7):2384-94
pubmed: 25616797
Water Resour Res. 2019 Mar;55(3):2104-2121
pubmed: 31068736
Arch Microbiol. 2021 Dec;203(10):5931-5936
pubmed: 34709419
Microb Ecol. 2017 Aug;74(2):264-277
pubmed: 28214969
Oecologia. 1979 Jan;40(1):51-62
pubmed: 28309603
Angew Chem Int Ed Engl. 2018 Feb 12;57(7):1758-1784
pubmed: 28815899
FEMS Microbiol Lett. 2012 Jul;332(1):68-75
pubmed: 22515452
J Appl Microbiol. 2015 Nov;119(5):1207-18
pubmed: 26184497
Appl Environ Microbiol. 1990 Sep;56(9):2623-9
pubmed: 16348272
Metabolomics. 2018 Feb 27;14(4):41
pubmed: 30830340
Int J Syst Evol Microbiol. 2018 Aug;68(8):2478-2484
pubmed: 29923816
Front Microbiol. 2018 Oct 15;9:2180
pubmed: 30374333
J Gen Microbiol. 1991 Nov;137(11):2631-41
pubmed: 1783909
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6
pubmed: 23193283
Int J Syst Evol Microbiol. 2017 Feb;67(2):396-401
pubmed: 27902279
Archaea. 2017 Jun 13;2017:2136287
pubmed: 28694737
ISME J. 2010 Dec;4(12):1581-90
pubmed: 20555365
Biochim Biophys Acta. 2008 Dec;1784(12):1873-98
pubmed: 18801467
Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12580-6
pubmed: 19617564
J Gen Microbiol. 1975 Feb;86(2):319-26
pubmed: 1113081
Rapid Commun Mass Spectrom. 2004;18(22):2650-60
pubmed: 15481101
Nat Commun. 2016 Oct 24;7:13219
pubmed: 27774985
Water Res. 2020 Mar 1;170:115341
pubmed: 31790889
Appl Environ Microbiol. 2019 Feb 20;85(5):
pubmed: 30578263
Chemosphere. 1997 Jul;35(1-2):275-94
pubmed: 9232001
Front Microbiol. 2016 Dec 19;7:2000
pubmed: 28066337
Environ Microbiol. 2022 Dec;24(12):5898-5910
pubmed: 36135934
Int J Syst Evol Microbiol. 2018 Jul;68(7):2331-2334
pubmed: 29809123
FEMS Microbiol Ecol. 2014 Oct;90(1):39-53
pubmed: 24953994
ISME J. 2017 May;11(5):1075-1086
pubmed: 28140394
Syst Appl Microbiol. 2001 Nov;24(3):377-84
pubmed: 11822673
Int J Syst Evol Microbiol. 2015 Sep;65(9):2865-2869
pubmed: 25991662
Int J Syst Evol Microbiol. 2011 Jul;61(Pt 7):1651-1655
pubmed: 20709913
Int J Syst Evol Microbiol. 2001 Jan;51(Pt 1):119-122
pubmed: 11211248
FEMS Microbiol Ecol. 2019 Jan 1;95(1):
pubmed: 30304451
Appl Environ Microbiol. 1983 Dec;46(6):1286-92
pubmed: 16346441
ISME J. 2016 Oct;10(10):2447-58
pubmed: 27022994
Front Microbiol. 2016 May 09;7:666
pubmed: 27242696
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2862-2866
pubmed: 20081014
FEBS J. 2005 Aug;272(16):4270-83
pubmed: 16098207