The pleiotropic functions of Pri smORF peptides synchronize leg development regulators.
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
14
03
2023
accepted:
03
10
2023
revised:
09
11
2023
medline:
13
11
2023
pubmed:
30
10
2023
entrez:
30
10
2023
Statut:
epublish
Résumé
The last decade witnesses the emergence of the abundant family of smORF peptides, encoded by small ORF (<100 codons), whose biological functions remain largely unexplored. Bioinformatic analyses here identify hundreds of putative smORF peptides expressed in Drosophila imaginal leg discs. Thanks to a functional screen in leg, we found smORF peptides involved in morphogenesis, including the pioneer smORF peptides Pri. Since we identified its target Ubr3 in the epidermis and pri was known to control leg development through poorly understood mechanisms, we investigated the role of Ubr3 in mediating pri function in leg. We found that pri plays several roles during leg development both in patterning and in cell survival. During larval stage, pri activates independently of Ubr3 tarsal transcriptional programs and Notch and EGFR signaling pathways, whereas at larval pupal transition, Pri peptides cooperate with Ubr3 to insure cell survival and leg morphogenesis. Our results highlight Ubr3 dependent and independent functions of Pri peptides and their pleiotropy. Moreover, we reveal that the smORF peptide family is a reservoir of overlooked developmental regulators, displaying distinct molecular functions and orchestrating leg development.
Identifiants
pubmed: 37903161
doi: 10.1371/journal.pgen.1011004
pii: PGENETICS-D-23-00283
pmc: PMC10635573
doi:
Substances chimiques
Drosophila Proteins
0
Peptides
0
Ubiquitin-Protein Ligases
EC 2.3.2.27
Ubr3 protein, Drosophila
EC 6.3.2.-
Tal protein, Drosophila
EC 2.2.1.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1011004Informations de copyright
Copyright: © 2023 Markus et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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